Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiatica L. Urban)
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Nguyen Loc
und
Nguyen Giang
Abstract
In this work, the effects of elicitor concentration and elicitation day on the growth and asiaticoside production of centella cells were investigated. The results showed that 2-hydroxybenzoic acid from 50–200 μM and yeast extract from 2–5 g L−1 had different eliciting influences. The addition of 2-hydroxybenzoic acid and yeast extract to the cultures strongly enhanced asiaticoside production in centella cells. The increase in asiaticoside content induced by the addition of 100 μM of 2-hydroxybenzoic acid and 4 g L−1 of yeast extract at day 10 of inoculation was about 5- and 3.5-fold, respectively, as compared with that of the reference cells. In general, 2-hydroxybenzoic acid (abiotic elicitor) was more effective in enhancing asiaticoside biosynthesis than yeast extract (biotic elicitor).
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© 2012 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Determination of sulphur species in solidified cryolite melts
- Separation of alicyclic and aromatic hydrocarbons on a PLOT column coated with 3-benzylketoiminepropyl group
- Analysis and improvement of stability of pepsin-solubilized collagen from skin of carp (Cyprinus carpio)
- Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiatica L. Urban)
- Increase of biogas production from pretreated hay and leaves using wood-rotting fungi
- Neural network model predictive control of a styrene polymerization plant: online testing using an electronic worksheet
- Influence of purine on copper behavior in neutral and alkaline sulfate solutions
- A parametric study on coal gasification for the production of syngas
- Tungstate sulfuric acid: preparation, characterization, and application in catalytic synthesis of novel benzimidazoles
- Synthesis of conjugated (E,E)-1,3-diene-containing troponoid-based compounds
- Reaction of aniline with ammonium persulphate and concentrated hydrochloric acid: Experimental and DFT studies
- Erratum to: “Zakaria Salmi, Sarra Gam-Derouich, Samia Mahouche-Chergui, Mireille Turmine, Mohamed M. Chehimi: On the interfacial chemistry of aryl diazonium compounds in polymer science”
