Collagen-grafted ultra-high molecular weight polyethylene for biomedical applications
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Jindřiška Bočková
,
Lucy Vojtová
Abstract
A novel material for hard tissue implants has been prepared. The ultra-high molecular weight polyethylene (UHMWPE) was grafted with collagen I, to improve its biocompatibility with soft tissue in case of its usage in bone engineering. Before collagen immobilization, commercial grade UHMWPE was treated with air plasma to introduce hydroperoxides onto the surface and subsequently grafted with carboxylic acid to functionalize the surface. Acrylic acid and itaconic acid were used for surface functionalization. After graft polymerization of carboxylic acids, collagen was immobilized covalently through the amide bonds between residual amino and carboxyl groups in the presence of water-soluble carbodiimide/hydroxysuccinimide cross-linking system. Each step of modification was characterized using spectroscopic (EPR, ATR-FTIR, and XPS), microscopic (SEM and CLSM), and contact angle measurement methods. The experimental results showed that plasma treatment led to a generation of free radicals on the UHMWPE surface resulting in the formation of unstable hydroperoxides. These reactive species were used to graft unsaturated carboxylic acids onto UHMWPE. Consequently, collagen was grafted via the-NH2 and-COOH reaction. The obtained experimental data along with microscopic observations confirmed the success of graft poly-merization of itaconic as well as of acrylic acid and collagen immobilization onto the UHMWPE surface.
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© 2008 Institute of Chemistry, Slovak Academy of Sciences
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- Photoswitching of a heptanuclear FeII-FeIII complex — A case of multifunctional magnetic materials
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- Synthesis of macrocyclic polyethers and polyether diesters and preparation of their cationic complexes
- Prediction of anti-HIV activity and cytotoxicity of pyrimidinyl and triazinyl amines: A QSAR study
- Composition of the essential oil of Geocaryum cynapioides (Guss.) L. Engstrand
- Photolytic degradation of triclosan in the presence of surfactants
- Determination of dissociation degrees of K3NbF8 and K3TaF8 by thermodynamic analysis of subsystems of the KF-K2NbF7 and KF-K2TaF7 systems
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