Segregated Model of Adherent Cell Culture in a Fixed-Bed Bioreactor
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Valérie Gelbgras
, Christophe E Wylock , Jean-Christophe Drugmand and Benoît Haut
In this work, a mathematical model of a fixed-bed bioreactor for the animal cell culture is developed to study the optimization and the scale-up of this bioreactor. Several cell populations are considered: the cells in suspension in the medium at the beginning of the process and the adhering cells to the fixed-bed. The model includes a capture rate kinetic of the cells in suspension by the fixed-bed and a spatial distribution of the nutrient and by-product concentrations in the fixed-bed. Therefore, the model reports the potential gradients of the cell concentrations in the fixed-bed. Some model parameters are experimentally identified and the model is validated using experimental data obtained with two pilot bioreactors. The model is used as a simulation tool to study the influence of the bioreactor design or the velocity field of the culture medium on the cell concentration gradients in the fixed-bed bioreactor and to optimize the operating conditions, the design, and the scale-up of this bioreactor.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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- Selection of Suitable Turbulence Models for Numerical Modelling of Hydrocyclones
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- Coupling Swelling and Water Retention Processes in Compacted Bentonite
- Adaptive Feedback Linearization Control of SISO Nonlinear Processes Using a Self-Generating Neural Network-Based Approach
- Solid-Liquid Equilibrium of Xylose in Water and Ethanol/Water Mixture
- Simulation of Hydrodesulfurization Trickle Bed Reactor
- Modeling Traffic-Emitted Ultrafine Particle Concentration and Intake Fraction in Corpus Christi, Texas
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- Modeling of Urea Release from Briquettes Using Semi infinite and Shrinking Core Models
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