Characterization of the Immobilized Fructosyltranferase from Rhodotorula sp.
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Elizama Aguiar-Oliveira
The fructosyltransferase from Rhodotorula sp. recovered from a fermented medium, by precipitation was immobilized by adsorption onto niobium ore. Considering the biocatalyst system, the activity/pH profile moved towards more alkaline values as compared to the free enzyme system, indicating that the support affected the charge distribution between the enzyme and the support. The immobilized enzyme showed high activity and good stability at pH values of 4.5 and 6.0. The biocatalyst half-lives at 48 °C and pH values of 4.5 and 6.0 were 32 and 72 days, respectively. The kinetics for the immobilized system corresponded to that of substrate inhibition. The synthesis of fructooligosaccharides from 50% sucrose solutions was carried out in batch stirred reactors and the conversion was about 58%, similar to that with the free enzyme. Based on the biocatalyst activity, stability and process yields, the system developed in this work can be considered suitable for application in large-scale bioreactors.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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- Thermal and Mechanical Effects on Retention of Food-Grade ?-carotene during Extrusion Processing
- Use of Pulsed Ultraviolet Light to Reduce the Allergenic Potency of Soybean Extracts
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- Shorter Communication
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Artikel in diesem Heft
- Article
- Thermophysical Characterization of Genipap Pulp
- Comparison of the Effects of Extrusion Cooking on Some Cereal Starches
- Extraction of Ginsenosides from American Ginseng (Panax quinquefolium L.) Root
- Inactivation of Polyphenoloxidase of Pear by Pulsed Electric Fields
- Artificial Neural Network Modelling of Heat Transfer to Canned Particulate Fluids under Axial Rotation Processing
- Ultrasound-Assisted Extraction in Different Solvents for Phytochemical Study of Canna indica
- Development and Characterization of Extruded Product Using Carrot Pomace and Rice Flour
- Characterization of the Immobilized Fructosyltranferase from Rhodotorula sp.
- Thermal and Mechanical Effects on Retention of Food-Grade ?-carotene during Extrusion Processing
- Use of Pulsed Ultraviolet Light to Reduce the Allergenic Potency of Soybean Extracts
- A Direct Process for the Production of High Fructose Syrups from Dates Extracts
- Optimization of Conditions for Collagen Extraction from the Swim Bladders of Grass Carp (Ctenopharyngodon idella) by Response Surface Methodology
- Proximate Composition and Rheological Properties of a Cake Mix Elaborated Using Composite Flour Wheat: Cassava
- Drying of Pomegranate Arils: Comparison of Predictions from Mathematical Models and Neural Networks
- Rheology of Selected Persian Honeys
- Shorter Communication
- Preparation of a Micro-Porous Alginate Gel Using a Microfluidic Bubbling Device
