Optimization of Conditions for Collagen Extraction from the Swim Bladders of Grass Carp (Ctenopharyngodon idella) by Response Surface Methodology
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Bao Zhang
, Yunzhong Chen , Xuefei Wei , Mingqi Li and Mengjin Wang
The effects of liquid-solid ratio, acetic acid concentration and extraction time on the yield of acid-soluble collagen(ASC) from the swim bladders of grass carp were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize the yield of ASC by implementing the Box-Wilson design. Statistical analysis of the results showed that the linear and quadric terms of these three variables had significant effects. However, no interactions between the three variables were found to contribute to the response at a significant level. The optimal conditions for higher yield of ASC were a liquid-solid ratio of 17.85, an acetic-acid concentration of 0.54 M and a time of 34 h. Under these conditions, the model predicted an ASC yield of 8.39%. Verification of the optimization showed that an ASC yield of 8.21±0.15% was observed under the optimal conditions. The experimental values agreed with the predicted values, using analysis of variance, indicating an excellent fit of the model used and the success of response surface methodology for modeling extraction of ASC from the swim bladders of grass carp.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Articles in the same Issue
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- Thermophysical Characterization of Genipap Pulp
- Comparison of the Effects of Extrusion Cooking on Some Cereal Starches
- Extraction of Ginsenosides from American Ginseng (Panax quinquefolium L.) Root
- Inactivation of Polyphenoloxidase of Pear by Pulsed Electric Fields
- Artificial Neural Network Modelling of Heat Transfer to Canned Particulate Fluids under Axial Rotation Processing
- Ultrasound-Assisted Extraction in Different Solvents for Phytochemical Study of Canna indica
- Development and Characterization of Extruded Product Using Carrot Pomace and Rice Flour
- Characterization of the Immobilized Fructosyltranferase from Rhodotorula sp.
- Thermal and Mechanical Effects on Retention of Food-Grade ?-carotene during Extrusion Processing
- Use of Pulsed Ultraviolet Light to Reduce the Allergenic Potency of Soybean Extracts
- A Direct Process for the Production of High Fructose Syrups from Dates Extracts
- Optimization of Conditions for Collagen Extraction from the Swim Bladders of Grass Carp (Ctenopharyngodon idella) by Response Surface Methodology
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- Rheology of Selected Persian Honeys
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