Climate resilience plastic degradation potential of Pseudomonas putida isolated from the soil of plastic waste dumping sites to reduce GHG emissions
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Hammad Majeed
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Tehreema Iftikhar
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Qamar Abbas
Abstract
Climate change is a universal issue and plastic trash is the major cause of global warming. The present study was specifically designed to address the escalating plastic waste problem, the biodegradation of low-density polyethylene (LDPE) bags was investigated over an 8-week period using liquid culture approach. Bacterial strain (Pseudomonas putida) capable of degrading LDPE was isolated from common plastic garbage dumping sites. The strain was identified after sequencing analysis, as P. putida was evaluated for its effectiveness in degrading LDPE. Various analytical techniques, including weight loss, SEM, FTIR and GC-MS were employed to assess the degradation process. Results revealed that bacterial strain achieved 0.0776 % degradation. SEM and FTIR confirmed surface and chemical changes, while GC MS identified degradation by products in comparison to the control (where no bacteria was used).
Acknowledgment
Authors wish to acknowledge Dr. Afshan Kaleem, Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan, for helping in molecular docking analysis.
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Research ethics: The authors declare that we have followed the research ethics strictly.
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Author contributions: Hammad Majeed: Research Concept, Significance, Write up, methodology, Evaluation; Tehreema Iftikhar: Research Concept, Main Supervision, Results and Evaluation, Discussion; Qamar Abbas: results compilation, characterization. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors state no conflict of interest.
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Research funding: None declared.
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Data availability: Not applicable.
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© 2024 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Papers
- Climate resilience plastic degradation potential of Pseudomonas putida isolated from the soil of plastic waste dumping sites to reduce GHG emissions
- Agricultural waste upcycling into improved production of triacyl glycerol acyl hydrolases
- Computational investigations of different iron oxide–coronene nanoclusters: a DFT study
- Fabrication, characterization, photocatalytic and biological performances of Mn/ZnO–SiO2 and ZnO–SiO2/PVA based ternary nanocomposites
- Ternary magnetic silica–graphene oxide composite for remediation of textile dyes from aqueous environment and real samples
- Biofabrication of silver nanoparticles using diallyl sulphide (DAS) and comparison of cytotoxic potential of DAS-AgNPs and Kodaikanal Hill garlic in human cervical cancer
- Green synthesis of AgNPs from leaves extract of Saliva Sclarea, their characterization, antibacterial activity, and catalytic reduction ability
- Effect of bead mill dispermat system with specific surfactants on organic pigments
Artikel in diesem Heft
- Frontmatter
- Original Papers
- Climate resilience plastic degradation potential of Pseudomonas putida isolated from the soil of plastic waste dumping sites to reduce GHG emissions
- Agricultural waste upcycling into improved production of triacyl glycerol acyl hydrolases
- Computational investigations of different iron oxide–coronene nanoclusters: a DFT study
- Fabrication, characterization, photocatalytic and biological performances of Mn/ZnO–SiO2 and ZnO–SiO2/PVA based ternary nanocomposites
- Ternary magnetic silica–graphene oxide composite for remediation of textile dyes from aqueous environment and real samples
- Biofabrication of silver nanoparticles using diallyl sulphide (DAS) and comparison of cytotoxic potential of DAS-AgNPs and Kodaikanal Hill garlic in human cervical cancer
- Green synthesis of AgNPs from leaves extract of Saliva Sclarea, their characterization, antibacterial activity, and catalytic reduction ability
- Effect of bead mill dispermat system with specific surfactants on organic pigments
