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Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line

  • Georgi G. Antov , Zlatina I. Gospodinova EMAIL logo , Miroslav Novakovic , Vele Tesevic , Natalia A. Krasteva , Danail V. Pavlov and Stefka V. Valcheva-Kuzmanova
Published/Copyright: September 30, 2024
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 80 Issue 5-6

Abstract

The aim of the present work was to investigate some of the molecular mechanisms and targets of the anticancer action of the bioflavonoid fustin isolated from the heartwood of Cotinus coggygria Scop. in the triple-negative breast cancer cell line MDA-MB-231. For this purpose, we applied fluorescence microscopy analysis to evaluate apoptosis, necrosis, and mitochondrial integrity, wound healing assay to study fustin antimigratory potential and quantitative reverse transcription-polymerase chain reaction to analyze the expression of genes associated with cell cycle control, programmed cell death, metastasis, and epigenetic alterations. A complex network-based bioinformatic analysis was also employed for protein–protein network construction, hub genes identification, and functional enrichment. The results revealed a significant induction of early and late apoptotic and necrotic events, a slight alteration of the mitochondria-related fluorescence, and marked antimotility effect after fustin treatment. Of 34 analyzed genes, seven fustin targets were identified, of which CDKN1A, ATM, and MYC were significantly enriched in pathways such as cell cycle, intrinsic apoptotic signaling pathway in response to DNA damage and generic transcription pathway. Our findings outline some molecular mechanisms of the anticancer action of fustin pointing it out as a potential oncotherapeutic agent and provide directions for future in vivo research.

Keywords: fustin; MDA-MB-231; proapoptotic effects; antimigratory potential; differentially expressed genes
Corresponding author: Zlatina I. Gospodinova, Laboratory of Genome Dynamics and Stability, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, 1113 Sofia, Bulgaria, E-mail:

Funding source: Bulgarian National Science Fund

Award Identifier / Grant number: KP-06-N43/6

  1. Research ethics: Not applicable.

  2. Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  4. Conflict of interest: The authors state no conflict of interest.

  5. Research funding: This work was funded by the Bulgarian National Science Fund, Ministry of Education and Science, grant number KP-06-N43/6 (КП-06-Н43/6/2020).

  6. Data availability: The raw data can be obtained on request from the corresponding author.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/znc-2024-0140).

Received: 2024-06-12
Accepted: 2024-09-10
Published Online: 2024-09-30
Published in Print: 2025-05-26

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2024-0140
Keywords for this article
fustin; MDA-MB-231; proapoptotic effects; antimigratory potential; differentially expressed genes

Articles in the same Issue

  1. Frontmatter
  2. Review Articles
  3. Nourishment beyond grains: unveiling the multifaceted contributions of millets to United Nations Sustainable Development Goals
  4. Exploring the functionality of fluorescent liposomes in cancer: diagnosis and therapy
  5. Research Articles
  6. In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii
  7. Characterization and antimicrobial activity of essential oils extracted from lemongrass (Cymbopogon flexuosus) using microwave-assisted hydro distillation
  8. In silico DFT and molecular modeling of novel pyrazine-bearing thiazolidinone hybrids derivatives: elucidating in vitro anti-cancer and urease inhibitors
  9. Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line
  10. Green synthesized AgNPs of the Anchusa arvensis aqueous extract resulting in impressive protein kinase, antioxidant, antibacterial, and antifungal activities
  11. Phytochemical composition, antimicrobial, antioxidant, and wound healing activities of Thermopsis turcica
  12. Molecular detection of Coxiella burnetii infection (Q fever) in livestock in Makkah Province, Saudi Arabia
  13. Spermidine protects cellular redox status and ionic homeostasis in D-galactose induced senescence and natural aging rat models
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