Home Phytochemical composition, antimicrobial, antioxidant, and wound healing activities of Thermopsis turcica
Article
Licensed
Unlicensed Requires Authentication

Phytochemical composition, antimicrobial, antioxidant, and wound healing activities of Thermopsis turcica

  • Gülçin Akdağ , Ömer Hazman , Laçine Aksoy ORCID logo EMAIL logo , Mehmet Savrık , Ahmet Büyükben , Mustafa Abdullah Yılmaz , Oguz Cakir and Recep Kara
Published/Copyright: October 21, 2024
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 80 Issue 5-6

Abstract

The antioxidant, antimicrobial, anticarcinogenic, wound healing activities and phenolic substance profile of aqueous extracts prepared using branch, leaf, flower parts and above-ground parts of Thermopsis turcica were determined in the study. The analyses indicate that the total phenolic substance contents and total antioxidant status are higher in the mix, flower, and leaf extracts. The extracts reduced cell viability in HGF cells more than in A549 cells. It shows that the extract has low anticarcinogenic activity in A549 cells. Flower extract had the highest wound closure rate. Quinic acid, cyranoside and luteolin were found in high concentrations in all extracts with LC/ESI-MS/LC analysis. It has been determined that the flower extract of the species is the most critical part showing antioxidant, antimicrobial, cytotoxic and wound healing properties. While the leaf and mix extracts stand out with their antioxidative and antimicrobial properties, the branch extract is effective in wound healing.

Keywords: Thermopsis turcica ; antioxidant; antimicrobial; cytotoxic; wound healing
Corresponding author: Laçine Aksoy, Department of Chemistry, Faculty of Science and Arts, Afyon Kocatepe University, 03200, Afyonkarahisar, Türkiye, E-mail:

  1. Research ethics: Not applicable.

  2. Informed consent: Not applicable.

  3. Author contributions: The extractions of plant and antioxidative, anticarcinogenic effect and wound healing assay experiments were performed with G.A., M.S., A.B., L.A. and Ö.H. phenolic content was determined by M.A.Y. and O.C. The antimicrobial activity experiment was made by R.K., L.A. wrote the article.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: All authors declared that there is no conflict of interest.

  6. Research funding: This work is supported by the Scientific Research Project Fund of Afyon Kocatepe University (AKU-BAP) under the Project number 23.FEN.BIL.02.

  7. Data availability: Not applicable.

References

1. Choi, IS, Choi, BH. The distinct plastid genome structure of Maackia fauriei (Fabaceae: Papilionoideae) and its systematic implications for genistoids and tribe Sophoreae. PLoS One 2017;12:e0173766. https://doi.org/10.1371/journal.pone.0173766.Search in Google Scholar PubMed PubMed Central

2. Cenkci, S, Kargıoğlu, M, Dayan, S, Konuk, M. Endangered status and propagation of an endemic plant species, Thermopsis turcica (Fabaceae). Asian J Plant Sci 2007;6:288–93. https://doi.org/10.3923/ajps.2007.288.293.Search in Google Scholar

3. Aksoy, L, Kolay, E, Ağılönü, Y, Aslan, Z, Kargıoğlu, M. Free radical scavenging activity, total phenolic content, total antioxidant status, and total oxidant status of endemic Thermopsis turcica. Saudi J Biol Sci 2013;20:235–9. https://doi.org/10.1016/j.sjbs.2013.02.003.Search in Google Scholar PubMed PubMed Central

4. Celik, Y, Küçükkurt, İ. Investigation of the antioxidant effects of extract obtained from Thermopsis turcica plant in rats. Kocatepe Vet J 2016;9:259–65. https://doi.org/10.5578/kvj.29164.Search in Google Scholar

5. Cenkci, S, Yıldız, M, Terzi, H. Thermopsis turcica, endemic to Afyonkarahisar: its past, today and gaining to economy. AKU J Sci 2012;12:23–6.Search in Google Scholar

6. Dorsett-Martin, WA. Rat models of skin wound healing: a review. Wound Repair Regen 2004;12:591–9. https://doi.org/10.1111/j.1067-1927.2004.12601.x.Search in Google Scholar PubMed

7. Mulkalwar, S, Behera, L, Golande, P, Manjare, R, Patil, H. Evaluation of wound healing activity of topical phenytoin in an excision wound model in rats. Int J Basic Clin Pharmacol 2015;4:139–43. https://doi.org/10.5455/2319-2003.ijbcp20150225.Search in Google Scholar

8. Lobo, V, Patil, A, Phatak, A, Chandra, N. Free radicals, antioxidants and functional foods: impact on human health. Phcog Rev 2010;4:118–26. https://doi.org/10.4103/0973-7847.70902.Search in Google Scholar PubMed PubMed Central

9. Lu, X, Gu, X, Shi, Y. A review on lignin antioxidants: their sources, isolations, antioxidant activities and various applications. Int J Biol Macromol 2022;210:716–41. https://doi.org/10.1016/j.ijbiomac.2022.04.228.Search in Google Scholar PubMed

10. Li, Q, Zhao, H, Chen, W, Huang, P. Berberine induces apoptosis and arrests the cell cycle in multiple cancer cell lines. Arch Med Sci 2023;19:1530–7. https://doi.org/10.5114/aoms/132969.Search in Google Scholar PubMed PubMed Central

11. Efferth, T, Saeed, MEM, Mirghani, E, Alim, A, Yassin, Z, Saeed, E, et al.. Integration of phytochemicals and phytotherapy into cancer precision medicine. Oncotarget 2017;8:50284–304. https://doi.org/10.18632/oncotarget.17466.Search in Google Scholar PubMed PubMed Central

12. Mohamed, EAA, Muddathir, AM, Osman, MA. Antimicrobial activity, phytochemical screening of crude extracts, and essential oils constituents of two Pulicaria spp. growing in Sudan. Sci Rep 2020;10:17148. https://doi.org/10.1038/s41598-020-74262-y.Search in Google Scholar PubMed PubMed Central

13. Hazman, O, Bozkurt, MF, Kumral, ZB, Savrık, M, Sindarov, B, Bhaya, MN, et al.. The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model. Biologia 2022;77:225–39.10.1007/s11756-022-01266-6Search in Google Scholar

14. Singleton, VL, Rossi, JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144–58. https://doi.org/10.5344/ajev.1965.16.3.144.Search in Google Scholar

15. Yilmaz, MA. Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: a detailed, robust and comprehensive LC–MS/MS method validation. Ind Crops Prod 2020;149:112347. https://doi.org/10.1016/j.indcrop.2020.112347.Search in Google Scholar

16. Bakir, D, Akdeniz, M, Ertas, A, Yilmaz, MA, Yener, İ, Firat, M, et al.. A GC–MS method validation for quantitative investigation of some chemical markers in Salvia hypargeia Fisch. C.A. Mey of Turkey: enzyme inhibitory potential of ferruginol. J Food Biochem 2020;44:e13350. https://doi.org/10.1111/jfbc.13350.Search in Google Scholar PubMed

17. Bauer, RW, Kirby, MDK, Sherris, JC, Turck, M. Antibiotic susceptibility testing by standard single disc diffusion method. Am J Clin Pathol 1966;45:493–6. https://doi.org/10.1093/ajcp/45.4_ts.493.Search in Google Scholar

18. Hassan, M, Javadzadeh, Y, Lotfipour, F, Badomchi, R. Determination of comparative minimum inhibitory concentration (MIC) of bacteriocins produced by enterococci for selected isolates of multi-antibiotic resistant Enterococcus spp. Adv Pharmaceut Bull 2011;1:75–9. https://doi.org/10.5681/apb.2011.011.Search in Google Scholar PubMed PubMed Central

19. Günay, E, Celik, S, Sarinc-Ulasli, S, Özyürek, A, Hazman, Ö, Günay, S, et al.. Comparison of the anti-inflammatory effects of proanthocyanidin, quercetin, and damnacanthal on benzo(a)pyrene exposed a549 alveolar cell line. Inflammation 2016;39:744–51. https://doi.org/10.1007/s10753-015-0301-3.Search in Google Scholar PubMed

20. Ahmad, S, Ahmad, A, Schneider, BK, White, CW. Cholesterol interferes with the MTT assay in human epithelial-Like (A549) and endothelial (HLMVE and HCAE) cells. Int J Toxicol 2006;25:17–23. https://doi.org/10.1080/10915810500488361.Search in Google Scholar PubMed

21. Mortenson, MM, Schlieman, MG, Virudachalam, S, Bold, RJ. Effects of the proteasome inhibitor bortezomib alone and in combination with chemotherapy in the A549 non-small-cell lung cancer cell line. Cancer Chemother Pharmacol 2004;54:343–53. https://doi.org/10.1007/s00280-004-0811-4.Search in Google Scholar PubMed

22. Rai, Y, Pathak, R, Kumari, N, Sah, DK, Pandey, S, Kalra, N, et al.. Mitochondrial biogenesis and metabolic hyperactivation limits the application of MTT assay in the estimation of radiation induced growth inhibition. Sci Rep 2018;8:1531. https://doi.org/10.1038/s41598-018-19930-w.Search in Google Scholar PubMed PubMed Central

23. Wei, W, Wang, L, Xu, L, Zeng, J. Anticancer mechanism of breviscapine in non-small cell lung cancer A549 cells acts via ROS-mediated upregulation of IGFBP4. J Thorac Dis 2021;13:2475–85. https://doi.org/10.21037/jtd-21-551.Search in Google Scholar PubMed PubMed Central

24. Ali, MM, Ciğerci, İH. Anti-Cancerous efficacy of alcoholic and aqueous extracts from an endemic plant Thermopsis turcica on Liver Carcinoma. Br J Pharmaceut Res 2017;16:1–5. https://doi.org/10.9734/bjpr/2017/32413.Search in Google Scholar

25. Kaptaner İğci, B, Aytaç, Z. An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve N. Robson Growing in Turkey. Turk J Pharm Sci 2020;17:610–9. https://doi.org/10.4274/tjps.galenos.2019.80037.Search in Google Scholar PubMed PubMed Central

26. Dai, J, Mumper, RJ. Plant phenolics: extraction, analysis and their vantioxidant and anticancer properties. Molecules 2010;15:7313–52. https://doi.org/10.3390/molecules15107313.Search in Google Scholar PubMed PubMed Central

27. Bali, EB, Açık, L, Akca, G, Sarper, M, Elçi, MP, Avcu, F, et al.. Antimicrobial activity against periodontopathogenic bacteria, antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica. Asian Pac J Trop Biomed 2014;4:505–14. https://doi.org/10.12980/apjtb.4.2014apjtb-2013-0010.Search in Google Scholar

28. Bandu, R, Ahn, HS, Lee, JW, Kim, YW, Choi, SH, Kim, HJ, et al.. Liquid chromatography electrospray ionization tandem mass spectrometric (LC/ESI-MS/MS) study for the identification and characterization of in vivo metabolites of cisplatin in rat kidney cancer tissues: online hydrogen/deuterium (H/D) exchange study. PLoS One 2015;10:e0134027. https://doi.org/10.1371/journal.pone.0134027.Search in Google Scholar PubMed PubMed Central

29. El Maaiden, E, Qarah, N, Ezzariai, A, Mazar, A, Nasser, B, Moustaid, K, et al.. Ultrasound-assisted extraction of isoquercetin from Ephedra alata (decne): optimization using response surface methodology and in vitro bioactivities. Antioxidants 2023;12:725. https://doi.org/10.3390/antiox12030725.Search in Google Scholar PubMed PubMed Central

30. Mbikay, M, Chrétien, M. Isoquercetin as an anti-covid-19 medication: a potential to realize. Front Pharmacol 2022;13:830205. https://doi.org/10.3389/fphar.2022.830205.Search in Google Scholar PubMed PubMed Central

31. Zhang, S, Gai, Z, Gui, T, Chen, J, Chen, Q, Li, Y. Antioxidant effects of protocatechuic acid and protocatechuic aldehyde: old wine in a new bottle. Evid Based Complement Alternat Med 2021;2021:6139308. https://doi.org/10.1155/2021/6139308.Search in Google Scholar PubMed PubMed Central

32. Chung, MJ, Kang, AY, Lee, KM, Oh, E, Jun, HJ, Kim, SY, et al.. Water-soluble genistin glycoside isoflavones up-regulate antioxidant metallothionein expression and scavenge free radicals. J Agric Food Chem 2006;54:3819–26. https://doi.org/10.1021/jf060510y.Search in Google Scholar

33. Yan, Y, Jun, C, Lu, Y, Jiangmei, S. Combination of metformin and luteolin synergistically protects carbon tetrachloride-induced hepatotoxicity: mechanism involves antioxidant, anti-inflammatory, antiapoptotic, and Nrf2/HO-1 signaling pathway. Biofactors 2019;45:598–606. https://doi.org/10.1002/biof.1521.Search in Google Scholar

34. Al-Megrin, WA, Alkhuriji, AF, Yousef, AOS, Metwally, DM, Habotta, OA, Kassab, RB, et al.. Antagonistic efficacy of luteolin against lead acetate exposure-associated with hepatotoxicity is mediated via antioxidant, anti-ınflammatory, and anti-apoptotic activities. Antioxidants 2019;9:10. https://doi.org/10.3390/antiox9010010.Search in Google Scholar

35. Zhang, H, Tan, X, Yang, D, Lu, J, Liu, B, Baiyun, R, et al.. Dietary luteolin attenuates chronic liver injury induced by mercuric chloride via the Nrf2/NF-kappaB/P53 signaling pathway in rats. Oncotarget 2017;8:40982–93. https://doi.org/10.18632/oncotarget.17334.Search in Google Scholar

36. Brown, PD, Ngeno, C. Antimicrobial resistance in clinical isolates of Staphylococcus aureus from hospital and community sources in southern Jamaica. Int J Infect Dis 2007;11:220–5. https://doi.org/10.1016/j.ijid.2006.04.005.Search in Google Scholar

37. Lou, Z, Wang, H, Zhu, S, Ma, C, Wang, Z. Antibacterial activity and mechanism of action of chlorogenic acid. J Food Sci 2011;76:398–403. https://doi.org/10.1111/j.1750-3841.2011.02213.x.Search in Google Scholar

38. Zaldivar, J, Ingram, LO. Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01. Biotechnol Bioeng 1999;66:203–10. https://doi.org/10.1002/(sici)1097-0290(1999)66:4<203::aid-bit1>3.3.co;2-r.10.1002/(SICI)1097-0290(1999)66:4<203::AID-BIT1>3.3.CO;2-RSearch in Google Scholar

39. Campos, FM, Couto, JA, Hogg, TA. Influence of phenolic acids on growth and inactivation of Oenococcus oeni and Lactobacillus hilgardii. J Appl Microbiol 2003;94:167–74. https://doi.org/10.1046/j.1365-2672.2003.01801.x.Search in Google Scholar

40. Khan, F, Niaz, K, Maqbool, F, Ismail Hassan, F, Abdollahi, M, Nagulapalli Venkata, KC, et al.. Molecular targets underlying the anticancer effects of quercetin: an update. Nutrients 2016;8:529. https://doi.org/10.3390/nu8090529.Search in Google Scholar

41. Asgharian, P, Tazekand, AP, Hosseini, K, Forouhandeh, H, Ghasemnejad, T, Ranjbar, M, et al.. Potential mechanisms of quercetin in cancer prevention: focus on cellular and molecular targets. Cancer Cell Int 2022;22:257. https://doi.org/10.1186/s12935-022-02677-w.Search in Google Scholar

42. Chen, Q, Li, P, Xu, Y, Li, Y, Tang, B. Isoquercitrin inhibits the progression of pancreatic cancer in vivo and in vitro by regulating opioid receptors and the mitogen-activated protein kinase signalling pathway. Oncol Rep 2015;33:840–8. https://doi.org/10.3892/or.2014.3626.Search in Google Scholar PubMed

43. Orfali, G, Duarte, AC, Bonadio, V, Martinez, NP, de Araújo, ME, Priviero, FB, et al.. Review of anticancer mechanisms of isoquercitin. World J Clin Oncol 2016;7:189–99. https://doi.org/10.5306/wjco.v7.i2.189.Search in Google Scholar PubMed PubMed Central

44. Riaz, A, Rasul, A, Hussain, G, Zahoor, MK, Jabeen, F, Subhani, Z, et al.. A bioactive phytochemical with potential therapeutic activities. Adv Pharmacol Sci 2018;2018:9794625. https://doi.org/10.1155/2018/2325659.Search in Google Scholar PubMed PubMed Central

45. Genc, S, Cicek, B, Yeni, Y, Hacımuftuoglu, A. Wound healing potential of quinic acid in human dermal fibroblasts by regulating the expression of FN1 and COL1α genes. Turkish J Nat Sci 2022;11:63–9.10.46810/tdfd.1186878Search in Google Scholar
Received: 2024-04-22
Accepted: 2024-09-18
Published Online: 2024-10-21
Published in Print: 2025-05-26

© 2024 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Review Articles
  3. Nourishment beyond grains: unveiling the multifaceted contributions of millets to United Nations Sustainable Development Goals
  4. Exploring the functionality of fluorescent liposomes in cancer: diagnosis and therapy
  5. Research Articles
  6. In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii
  7. Characterization and antimicrobial activity of essential oils extracted from lemongrass (Cymbopogon flexuosus) using microwave-assisted hydro distillation
  8. In silico DFT and molecular modeling of novel pyrazine-bearing thiazolidinone hybrids derivatives: elucidating in vitro anti-cancer and urease inhibitors
  9. Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line
  10. Green synthesized AgNPs of the Anchusa arvensis aqueous extract resulting in impressive protein kinase, antioxidant, antibacterial, and antifungal activities
  11. Phytochemical composition, antimicrobial, antioxidant, and wound healing activities of Thermopsis turcica
  12. Molecular detection of Coxiella burnetii infection (Q fever) in livestock in Makkah Province, Saudi Arabia
  13. Spermidine protects cellular redox status and ionic homeostasis in D-galactose induced senescence and natural aging rat models
https://doi.org/10.1515/znc-2024-0102
Keywords for this article
Thermopsis turcica; antioxidant; antimicrobial; cytotoxic; wound healing

Articles in the same Issue

  1. Frontmatter
  2. Review Articles
  3. Nourishment beyond grains: unveiling the multifaceted contributions of millets to United Nations Sustainable Development Goals
  4. Exploring the functionality of fluorescent liposomes in cancer: diagnosis and therapy
  5. Research Articles
  6. In vitro antibacterial activities, DPPH radical scavenging, and molecular simulation of isolated compounds from the leaves of Rhus ruspolii
  7. Characterization and antimicrobial activity of essential oils extracted from lemongrass (Cymbopogon flexuosus) using microwave-assisted hydro distillation
  8. In silico DFT and molecular modeling of novel pyrazine-bearing thiazolidinone hybrids derivatives: elucidating in vitro anti-cancer and urease inhibitors
  9. Molecular mechanisms of the anticancer action of fustin isolated from Cotinus coggygria Scop. in MDA-MB-231 triple-negative breast cancer cell line
  10. Green synthesized AgNPs of the Anchusa arvensis aqueous extract resulting in impressive protein kinase, antioxidant, antibacterial, and antifungal activities
  11. Phytochemical composition, antimicrobial, antioxidant, and wound healing activities of Thermopsis turcica
  12. Molecular detection of Coxiella burnetii infection (Q fever) in livestock in Makkah Province, Saudi Arabia
  13. Spermidine protects cellular redox status and ionic homeostasis in D-galactose induced senescence and natural aging rat models
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 6.11.2025 from https://www.degruyterbrill.com/document/doi/10.1515/znc-2024-0102/html?lang=en
Scroll to top button