Isolation of compounds from the roots of Ambrosia artemisiifolia and their effects on human cancer cell lines
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Elek Ferencz
,
István Zupkó
Abstract
Common ragweed (Ambrosia artemisiifolia L.) is an invasive plant in Europe with spreading use in the contemporary folk medicine. The chemical composition of the above-ground parts is extensively studied, however, the metabolites of the roots are less discovered. By multiple chromatographic purification of the root extracts, we isolated thiophene A (1), n-dodecene (2), taraxerol-3-O-acetate (3), α-linoleic acid (4), (+)-pinoresinol (5), and thiophene E (7,10-epithio-7,9-tridecadiene-3,5,11-triyne-1,2-diol) (6). The 1H NMR data published earlier for 1 were supplemented together with the assignment of 13C NMR data. Thiophene E (6), which is reported for the first time from this species, exerted cytotoxic and antiproliferative effects on A-431 epidermoid skin cancer cells, whereas taraxerol-3-O-acetate (3) and α-linoleic acid (4) had slight antiproliferative effect on gynecological cancer cell lines. Thiophene E (6) and taraxerol-3-O-acetate (3) displayed antiproliferative and cytotoxic effects on MRC-5 fibroblast cells. Thiophene E (6) exerted weak antibacterial activity (MIC 25 μg/mL) on MRSA ATCC 43300, on Staphylococcus aureus ATCC 25923, Escherichia coli AG100 and E. coli ATCC 25922 both thiophenes were inactive. Although the isolated compounds exerted no remarkable cytotoxic or antiproliferative activities, the effects on MRC-5 fibroblast cells highlight the necessity of further studies to support the safety of ragweed root.
Funding source: National Research, Development and Innovation Office, Hungary
Award Identifier / Grant number: NKFIH K135845
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Author contributions: Boglárka Csupor-Löffler, István Zupkó and Gabriella Spengler contributed to the study conception and design. Material preparation, data collection and analysis were performed by Judit Hohmann, Norbert Kúsz, Martin Vollár, Eszter Laczkó-Zöld and Elek Ferencz. Laboratory experiments were performed by Elek Ferencz, Boglárka Csupor-Löffler, Martin Vollár, Zoltán Péter Zomborszki, Balázs Kovács and Gabriella Spengler. The first draft of the manuscript was written by Boglárka Csupor-Löffler, István Zukó, Judit Hohmann, Dezső Csupor and Gabriella Spengler and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: This research was funded by the National Research, Development and Innovation Office, Hungary, grant number NKFIH K135845.
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Conflict of interest statement: The authors declare no conflict of interest.
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Supplementary Material
This article contains supplementary material (https://doi.org/10.1515/znc-2022-0239).
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Artikel in diesem Heft
- Frontmatter
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- Structure elucidation of an aspidofractinine-type monoterpene indole alkaloid from Melodinus reticulatus
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- Isolation of compounds from the roots of Ambrosia artemisiifolia and their effects on human cancer cell lines
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Artikel in diesem Heft
- Frontmatter
- Research Articles
- Proximate analysis and fatty acid, mineral and soluble carbohydrate profiles of some brown macroalgae collected from Türkiye coasts
- Structure elucidation of an aspidofractinine-type monoterpene indole alkaloid from Melodinus reticulatus
- Crotofoligandrin, a new endoperoxide crotofolane-type diterpenoid from the twigs of Croton oligandrus Pierre ex. Hutch (Euphorbiaceae)
- Chemical composition of different plant part from Lactuca serriola L. – focus on volatile compounds and fatty acid profile
- Essential oil composition, anti-tyrosinase activity, and molecular docking studies of Knema intermedia Warb. (Myristicaceae)
- Isolation of compounds from the roots of Ambrosia artemisiifolia and their effects on human cancer cell lines
- Berberine may provide redox homeostasis during aging in rats
- The search for commercial sweet white lupin (Lupinus albus L.) adaptive to Ethiopian growing condition seems not successful: what should be done?
