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Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects

  • Gülsen Tel-Çayan ORCID logo EMAIL logo , Ebru Deveci , Zeynep Molo , Mehmet Emin Duru and Mehmet Öztürk
Published/Copyright: July 6, 2021
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 77 Issue 1-2

Abstract

Isolation and bioactive effects of the roots of Chaerophyllum bulbosum L. were firstly investigated herein. Enzyme (acetylcholinesterase, butyrylcholinesterase, urease, α-amylase, α-glucosidase, and tyrosinase) inhibitory effects of C. bulbosum root extracts were tested. Three known compounds, n-heptadecanyl eicosanoate (1), stigmasterol (2), and β-sitosterol-3-O-β-d-glucopyranoside (3) were isolated from C. bulbosum. Antioxidant and enzyme inhibitory effects of isolated compounds were investigated. The hexane extract (IC50: 349.58 ± 0.06 μg/mL) displayed a higher α-glucosidase inhibitory effect than the standard (IC50: 378.66 ± 0.14 μg/mL). The best inhibitory effect was found in compound 2 on AChE (46.40 ± 0.31%), BChE (56.41 ± 0.54%), and urease (92.47 ± 0.11%); compound 1 on α-amylase (22.27 ± 0.61%); and compound 3 on α-glucosidase (12.43 ± 0.25%) and tyrosinase (19.00 ± 0.16%). All isolated compounds showed moderate antioxidant effects in all assays. This study contributes to the therapeutic uses of Chaerophyllum roots and emphasizes the value of C. bulbosum species for the development of novel therapeutic agents.

Keywords: antioxidant effect; C. bulbosum ; enzyme inhibitory effect; isolation; roots
Corresponding author: Gülsen Tel-Çayan, Department of Chemistry and Chemical Processing Technologies, Muğla Vocational School, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey, E-mail:

Funding source: The Scientific and Technological Research Council of Turkey

Award Identifier / Grant number: TUBITAK-118Z610

Acknowledgments

The authors would like to thank Dr. Yeter Yeşil for the identification of the plant sample.

  1. Author contributions: G.T.Ç. designed, tested the biological assays, assisted in the isolation of compounds, resolved the structures of the compounds, analyzed data, supervised the project, and wrote the manuscript. E.D. performed the extraction, tested the biological assays, carried out the isolation of compounds, and wrote the manuscript. Z.M. performed the extraction, tested the biological assays, and carried out the isolation of compounds. M.E.D. analyzed data and contributed to the experiments. M.Ö. resolved the structures of the compounds.

  2. Research funding: This study was supported financially by The Scientific and Technological Research Council of Turkey (TUBITAK-118Z610).

  3. Conflict of interest statement: The authors declare no conflict of interest.

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Supplementary material

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2021-0119).

Received: 2021-04-18
Accepted: 2021-06-15
Published Online: 2021-07-06
Published in Print: 2022-01-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Research Articles
  3. Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects
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  8. A synergy interaction of artocarpin and tetracycline against Pseudomonas aeruginosa and its mechanism of action on membrane permeability
  9. Review Articles
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https://doi.org/10.1515/znc-2021-0119
Keywords for this article
antioxidant effect; C. bulbosum; enzyme inhibitory effect; isolation; roots

Articles in the same Issue

  1. Frontmatter
  2. Research Articles
  3. Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects
  4. Monascin and monascinol, azaphilonoid pigments from Mortierella polycephala AM1: in silico and in vitro targeting of the angiogenic VEGFR2 kinase
  5. Novel Ag(I)-NHC complex: synthesis, in vitro cytotoxic activity, molecular docking, and quantum chemical studies
  6. NLRP3 inflammasome activation and oxidative stress status in the mild and moderate SARS-CoV-2 infected patients: impact of melatonin as a medicinal supplement
  7. Oxidative stress and cyto-genotoxicity induced by poly-d-glucosamine in human blood cells in vitro
  8. A synergy interaction of artocarpin and tetracycline against Pseudomonas aeruginosa and its mechanism of action on membrane permeability
  9. Review Articles
  10. Cytokine storm syndrome in SARS-CoV-2: a review
  11. A comprehensive review on the development of probiotic supplemented confectioneries
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