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Oxidative stress and cyto-genotoxicity induced by poly-d-glucosamine in human blood cells in vitro

  • Salim Cerig ORCID logo EMAIL logo and Fatime Geyikoglu
Published/Copyright: May 26, 2021
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 77 Issue 1-2

Abstract

Poly-N-acetyl-d-glucosamine (CH; chitin) is the main component of the insect skeleton, fungal cell wall, and many crustaceans, including crab and shrimp. CH is the most abundant in nature after cellulose, and it has a complex and hardly soluble structure. Poly-d-glucosamine (CHO; chitosan) is a soluble derivative of CH produced by deacetylation used in many fields, including human health. This study carried out the cytotoxic, genotoxic, and oxidative effects of CHO on human whole blood (hWB) and lymphocytes (LYMs) in dose ranges 6.25–2000 μg/mL, in vitro. Total antioxidant capacity (TAC) and total oxidant status (TOS) analyzes were performed on plasma to appreciate oxidative stress. 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were applied to understand the cytotoxicity. Chromosomal aberration (CA) and micronucleus (MN) methods were practiced to evaluate genotoxicity. 6.25–150 μg/mL doses increased TAC and decreased TOS. A decreasing and increasing curve from 200 to 2000 μg/mL on TAC and TOS values were determined, respectively. 0–250 μg/mL doses did not provide any cytotoxic data. However, 500–2000 μg/mL doses showed increasing cytotoxicity and genotoxicity. The study results showed that CHO does not pose a toxic risk to human health at low doses but may pose a threat at high doses.

Keywords: human blood; oxidative stress; poly-D-glucosamine; seafood; toxicity
Corresponding author: Salim Cerig, First and Emergency Aid Program, Medical Services and Techniques Department, Vocational School of Health Services, Ibrahim Cecen University, Agri, Turkey, E-mail:

Funding source: Scientific Research Project, Ataturk University

Award Identifier / Grant number: 2014/175

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was financially supported by Scientific Research Project (accepted in 2014, Project No: 2014/175) from Ataturk University.

  3. Conflict of interest statement: The authors have no potential conflicts of interest for the research, authorship, and/or publication of this article.

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Received: 2021-03-18
Accepted: 2021-05-02
Published Online: 2021-05-26
Published in Print: 2022-01-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2021-0080
Keywords for this article
human blood; oxidative stress; poly-D-glucosamine; seafood; toxicity

Articles in the same Issue

  1. Frontmatter
  2. Research Articles
  3. Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects
  4. Monascin and monascinol, azaphilonoid pigments from Mortierella polycephala AM1: in silico and in vitro targeting of the angiogenic VEGFR2 kinase
  5. Novel Ag(I)-NHC complex: synthesis, in vitro cytotoxic activity, molecular docking, and quantum chemical studies
  6. NLRP3 inflammasome activation and oxidative stress status in the mild and moderate SARS-CoV-2 infected patients: impact of melatonin as a medicinal supplement
  7. Oxidative stress and cyto-genotoxicity induced by poly-d-glucosamine in human blood cells in vitro
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