Fisetin effects on cell proliferation and apoptosis in glioma cells
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Fulya Pak
Abstract
This research investigated the antiproliferative effects of 1–500 μM fisetin in T98G and BEAS-2B cells by MTT assay. The IC50 of fisetin in T98G cells for 24 and 48 h were 93 and 75 μM, respectively. Apoptotic alterations of fisetin-treated T98G cells were observed by transmission electron microscopy. BEAS-2B was then used in comparison to T98G cells to determine the cytotoxic effects of fisetin. The IC50 of fisetin for 24 and 48 h were recorded as 270 and 90 μM in BEAS-2B cells, respectively. Different concentrations of fisetin were selected to determine the apoptotic and necrotic effects. Consequently, fisetin was determined to have more apoptotic effects in T98G than BEAS-2B cells, dose- and time-dependently. Moreover, fisetin was found to have cytotoxicity at lower doses in T98G cells compared to carmustine, as positive control. CASPASE 3, CASPASE 9, CASPASE 8, and BAX expressions were increased by the selected fisetin doses of 25 and 50 μM, while that of BCL-2 and survivin was reduced in T98G cells. These results will serve as an essential basis of future in vitro and in vivo studies, in the continuous search for alternative treatment agents for gliomas.
Acknowledgements
This publication was based completely on M.Sc. thesis of the first author. The study was supported by Eskisehir Osmangazi University Scientific Research Projects Committee (project no. 201319A112).
Conflict of interest: The authors declare no conflict of interest.
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Research Articles
- Secondary metabolites from Penicillium sp. 8PKH isolated from deteriorated rice straws
- A new ursane-type triterpene oxoglucopyranoside from Crossopteryx febrifuga
- Fisetin effects on cell proliferation and apoptosis in glioma cells
- Cytotoxic and anti-inflammatory resorcinol and alkylbenzoquinone derivatives from the leaves of Ardisia sieboldii
- Isolation and characterization of antiplasmodial constituents from the marine sponge Coscinoderma sp.
- The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae
- Hepatoprotective activity of a purified methanol extract and saponins from the roots of Chenopodium bonus-henricus L.
Articles in the same Issue
- Frontmatter
- Research Articles
- Secondary metabolites from Penicillium sp. 8PKH isolated from deteriorated rice straws
- A new ursane-type triterpene oxoglucopyranoside from Crossopteryx febrifuga
- Fisetin effects on cell proliferation and apoptosis in glioma cells
- Cytotoxic and anti-inflammatory resorcinol and alkylbenzoquinone derivatives from the leaves of Ardisia sieboldii
- Isolation and characterization of antiplasmodial constituents from the marine sponge Coscinoderma sp.
- The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae
- Hepatoprotective activity of a purified methanol extract and saponins from the roots of Chenopodium bonus-henricus L.
