Hepatoprotective activity of a purified methanol extract and saponins from the roots of Chenopodium bonus-henricus L.
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Abstract
An ultra-high-performance liquid chromatography-high-resolution mass spectrometry based profiling of a purified MeOH extract (PME) from the roots of Chenopodium bonus-henricus L. (Amaranthaceae) tentatively identified 15 saponins of six sapogenins. The PME exerts hepatoprotective and antioxidant activities comparable to those of flavonoid complex silymarin in in vitro (1 and 10 μg/mL) and in vivo (200 mg/kg/daily for 7 days) models of hepatotoxicity, induced by CCl4. The main constituents of PME, respectively saponins bonushenricoside A (1), 3-O-β-D-glucuronopyranosyl-bayogenin-28-O-β-D-glucopyranosyl ester (2), 3-O-β-D-glucuronopyranosyl-medicagenic acid-28-O-β-D-xylopyranosyl (1→4)-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl ester (3), 3-O-β-D-glucuronopyranosyl-2β-hydroxygypsogenin-28-O-β-D-glucopyranosyl ester (4), 3-O-α-L-rabinopyranosyl-bayogenin-28-O-β-D-glucopyranosyl ester (6) and bonushenricoside B (8) (3 μg/mL each), compared to silymarin (5 and 50 μg/mL), significantly reduced the cellular damage caused by CCl4 in rat hepatocytes, preserved cell viability and glutathione level, decreased lactate dehydrogenase leakage and reduced lipid damage. The experimental data suggest that the glycosides of phytolaccagenin, bayogenin, medicagenic acid, 2β-hydroxygypsogenin, 2β-hydroxyoleanoic acid and oleanoic acid are a promising and safe class of hepatoprotective agents.
Funding source: Council of Medical Science of the Medical University of Sofia
Award Identifier / Grant number: 33/2013
Funding statement: This work was supported by the Council of Medical Science of the Medical University of Sofia (Grand 33/2013).
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Secondary metabolites from Penicillium sp. 8PKH isolated from deteriorated rice straws
- A new ursane-type triterpene oxoglucopyranoside from Crossopteryx febrifuga
- Fisetin effects on cell proliferation and apoptosis in glioma cells
- Cytotoxic and anti-inflammatory resorcinol and alkylbenzoquinone derivatives from the leaves of Ardisia sieboldii
- Isolation and characterization of antiplasmodial constituents from the marine sponge Coscinoderma sp.
- The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae
- Hepatoprotective activity of a purified methanol extract and saponins from the roots of Chenopodium bonus-henricus L.
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Secondary metabolites from Penicillium sp. 8PKH isolated from deteriorated rice straws
- A new ursane-type triterpene oxoglucopyranoside from Crossopteryx febrifuga
- Fisetin effects on cell proliferation and apoptosis in glioma cells
- Cytotoxic and anti-inflammatory resorcinol and alkylbenzoquinone derivatives from the leaves of Ardisia sieboldii
- Isolation and characterization of antiplasmodial constituents from the marine sponge Coscinoderma sp.
- The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae
- Hepatoprotective activity of a purified methanol extract and saponins from the roots of Chenopodium bonus-henricus L.
