Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici
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Amena Khatun
Abstract
The objective of this study was to isolate and characterize antagonistic rhizobacteria from chili against a notorious phytopathogen Phytophthora capsici. Among the 48 bacteria isolated, BTLbbc-02, BTLbbc-03, and BTLbbc-05 were selected based on their inhibitory activity against P. capsici. They were tentatively identified as Burkholderia metallica BTLbbc-02, Burkholderia cepacia BTLbbc-03, and Pseudomonas aeruginosa BTLbbc-05, respectively, based on their 16S rRNA gene sequencing. All inhibited the growth of P. capsici at varying levels by inducing characteristic morphological alterations of P. capsici hyphae. The cell-free culture supernatant of all three isolates impaired motility (up to 100%) and caused lysis (up to 50%) of the halted zoospores. Bioassays revealed that Pseudomonas sp. had higher antagonism and zoospore motility-inhibitory effects against P. capsici compared with two other isolates, Burkholderia spp. and B. metallica, which caused vacuolation in mycelium. All three bacteria suppressed sporangium formation and zoosporogenesis of P. capsici, and improved the seed germination and growth of cucumber. Our findings suggest that epiphytic bacteria, B. metallica, B. cepacia, and P. aeruginosa, could be used as potential biocontrol agents against P. capsici. A further study is required to ensure conformity with the existing regulations for soil, plant, and human health.
Acknowledgment
The authors are thankful to the World Bank for funding to CP #2071 (Funder ID: 10.13039/100004421) of Higher Education Quality Enhancement Project of University Grants Commission of Bangladesh. Sincere thanks are also due to W. Yuanchao for generously providing the P. capsici strain.
Competing interests: The authors have declared that there are no conflicts of interests that could be observed as influencing the impartiality of this paper.
Author contributions: A.K. performed experiments, analyzed data, and drafted the manuscript. T.F. and A.A.S performed data collection. T.I., S.M.N.I., M.R., and H.M.W. supervised the design of the study and data analysis, and revised the manuscript. All authors critically reviewed the manuscript for intellectual content and gave final approval for the version to be published.
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The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2017-0065).
©2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
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Artikel in diesem Heft
- Frontmatter
- Fast RBC loading by fluorescent antibodies and nuclei staining dye and their potential bioanalytical applications
- Chemical profiling of Di-Wu-Yang-Gan Granules by ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with MSE technology
- Design, synthesis, and antibacterial activity of novel 8-methoxyquinoline-2-carboxamide compounds containing 1,3,4-thiadiazole moiety
- Pseudomonas and Burkholderia inhibit growth and asexual development of Phytophthora capsici
- Antiproliferative activity of synthesized some new benzimidazole carboxamidines against MCF-7 breast carcinoma cells
- Overexpression of c-Myc enhances recombinant protein production in High Five cells after baculovirus infection
- Kostchyienones A and B, new antiplasmodial and cytotoxicity of limonoids from the roots of Pseudocedrela kotschyi (Schweinf.) Harms
- Antimicrobial and antioxidant activities of two polyketides from lichen-endophytic fungus Preussia sp.
