Optimization of the primary purification process of extracting sphorolipid from the fermentation broth to achieve a higher yield and purity
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Yogita Pal
Yogita Pal: She was student at the Institute of Chemical Technology (University under Section 3 of UGC Act 1956, Formerly UDCT/UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400019, India.
Suraj N. Mali: completed his M. Pharm (Pharmaceutical Chemistry) at Institute of Chemical Technology, Mumbai in 2019. He has completed his B. Pharm at Government College of Pharmacy, Karad, India. Then, he joined Dr. Reddy’s Lab, Hyderabad as analytical scientist. He has 61+ international publications of repute (Citations: 463; H-index: 14) at his credit in the field of drug discovery, medicinal chemistry, computer-aided drug design, and synthetic/organic chemistry. He is also associate editor member of Journal, “Current Indian Science” (Bentham Science Publisher).
Abstract
Sophorolipid (SL) is a surface-active glycolipid biosurfactant with promising industrial applications. It is synthesised by fermentation of hydrophobic and hydrophilic substrates using selected non-pathogenic yeasts. However, its applications are limited by high production costs and ineffective product recovery in downstream purification stages. Natural sophorolipids are produced in six to nine different hydrophobic sophorosides, where the carboxyl end of the fatty acid is either free, which is known as the acidic or open form, or it can be esterified internally to produce the lactonic form. The present study deals with the screening and selection of suitable solvents for the extraction of acidic and lactonic SL from fermentation broth. The optimisation study involves exhaustive extraction with the six different immiscible solvents ethyl acetate, butyl acetate, methylene dichloride, methyl tert.-butyl ether, methyl iso-butyl ketone and methyl ethyl ketone. The partition coefficient (Kd), which is the ratio of the solute concentration in the organic layer compared to the aqueous layer, determines the performance measurement of the extraction process in terms of yield and purity of the desired solute. The factors that influence exhaustive extraction were the broth to solvent ratio and the extraction stages. The optimal extraction conditions for the highest possible yield were a broth to solvent ratio of 1:1 and a number of extraction steps of 2. Methylene dichloride showed better results in terms of yield and selectivity in the extraction of acidic and lactonic SL from the fermentation broth compared to the other solvents investigated. For lactonic SL, the highest Kd value determined was 36.6 and for acidic SL the highest Kd value was 1.14.
About the authors
Yogita Pal: She was student at the Institute of Chemical Technology (University under Section 3 of UGC Act 1956, Formerly UDCT/UICT), Nathalal Parekh Marg, Matunga (East), Mumbai 400019, India.
Suraj N. Mali: completed his M. Pharm (Pharmaceutical Chemistry) at Institute of Chemical Technology, Mumbai in 2019. He has completed his B. Pharm at Government College of Pharmacy, Karad, India. Then, he joined Dr. Reddy’s Lab, Hyderabad as analytical scientist. He has 61+ international publications of repute (Citations: 463; H-index: 14) at his credit in the field of drug discovery, medicinal chemistry, computer-aided drug design, and synthetic/organic chemistry. He is also associate editor member of Journal, “Current Indian Science” (Bentham Science Publisher).
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: None declared.
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Conflict of interest statement: Authors declare that they do not have any conflicts of interest.
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- The effect of tablet detergent wastewater using zeolite from rice husk ash on the chemical water quality and the growth of water hyacinth
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Articles in the same Issue
- Frontmatter
- Review
- Surfactant as an anti-corrosive agent: a review
- Applications
- Formulation and evaluation of a novel cubosomal emulgel for topical delivery of luliconazole
- Study on graphene-based emulsions as oil displacement agent
- Role of mixed surfactants system in preparation of silver nanoparticles
- Physical Chemistry
- Phase behavior of the sodium lauryl glutamate: effects of the temperature and concentration
- Synthesis
- Preparation of carbosilane quaternary ammonium surfactants and surface activity
- Detergent Ingredients
- The effect of tablet detergent wastewater using zeolite from rice husk ash on the chemical water quality and the growth of water hyacinth
- Biosurfactants
- Optimization of the primary purification process of extracting sphorolipid from the fermentation broth to achieve a higher yield and purity
