Let there be light: zebrafish neurobiology and the optogenetic revolution
-
Claire Wyart
und
Filippo Del Bene
Abstract
Optogenetics has revolutionized the toolbox arsenal that neuroscientists now possess to investigate neuronal circuit function in intact and living animals. With a combination of light emitting ‘sensors’ and light activated ‘actuators’, we can monitor and control neuronal activity with minimal perturbation and unprecedented spatiotemporal resolution. Zebrafish neuronal circuits represent an ideal system to apply an optogenetic based analysis owing to its transparency, relatively small size and amenability to genetic manipulation. In this review, we describe some of the most recent advances in the development and applications of optogenetic sensors (i.e., genetically encoded calcium indicators and voltage sensors) and actuators (i.e., light activated ion channels and ion pumps). We focus mostly on the tools that have already been successfully applied in zebrafish and on those that show the greatest potential for the future. We also describe crucial technical aspects to implement optogenetics in zebrafish including strategies to drive a high level of transgene expression in defined neuronal populations, and recent optical advances that allow the precise spatiotemporal control of sample illumination.
©2011 by Walter de Gruyter Berlin New York
Artikel in diesem Heft
- Publisher's Note
- A new start for Reviews in the Neurosciences
- Guest Editorial
- A small fish with a big future: zebrafish in behavioral neuroscience
- Application of zebrafish oculomotor behavior to model human disorders
- Shoaling in zebrafish: what we don’t know
- Sleep and its regulation in zebrafish
- Zebrafish behavioural assays of translational relevance for the study of psychiatric disease
- Stressing zebrafish for behavioral genetics
- Imaging escape and avoidance behavior in zebrafish larvae
- Zebrafish assessment of cognitive improvement and anxiolysis: filling the gap between in vitro and rodent models for drug development
- Alcohol-induced behavior change in zebrafish models
- Zebrafish models to study drug abuse-related phenotypes
- The role of dopaminergic signalling during larval zebrafish brain development: a tool for investigating the developmental basis of neuropsychiatric disorders
- Let there be light: zebrafish neurobiology and the optogenetic revolution
Artikel in diesem Heft
- Publisher's Note
- A new start for Reviews in the Neurosciences
- Guest Editorial
- A small fish with a big future: zebrafish in behavioral neuroscience
- Application of zebrafish oculomotor behavior to model human disorders
- Shoaling in zebrafish: what we don’t know
- Sleep and its regulation in zebrafish
- Zebrafish behavioural assays of translational relevance for the study of psychiatric disease
- Stressing zebrafish for behavioral genetics
- Imaging escape and avoidance behavior in zebrafish larvae
- Zebrafish assessment of cognitive improvement and anxiolysis: filling the gap between in vitro and rodent models for drug development
- Alcohol-induced behavior change in zebrafish models
- Zebrafish models to study drug abuse-related phenotypes
- The role of dopaminergic signalling during larval zebrafish brain development: a tool for investigating the developmental basis of neuropsychiatric disorders
- Let there be light: zebrafish neurobiology and the optogenetic revolution
