Dissecting circuit mechanisms by genetic manipulation of specific neural pathways
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Shigeki Kato
Shigeki Kato completed a BSc and PhD (Agr.) at the Laboratory of Molecular Biology, Graduate School of Agricultural Science in Tohoku University. Then he took a postdoctoral position in Fukushima Medical University with Professor Kazuto Kobayashi, and is now an Assistant Professor. In 2012, he was awarded a PhD (Med.) at Fukushima Medical University. His principal research interests include how the neural circuit is controlled to conduct a variety of behaviors using genetic manipulation of neural circuit with the retrograde gene transfer vector.
Kenta Kobayashi graduated from Department of Agricultural Chemistry in Kyoto University, and received a PhD in bioscience from Nara Institute of Science and Technology. He worked as Assistant Professor in Fukushima Medical University, and is an currently Associate Professor at the National Institute of Physiological Sciences, Okazaki, Japan. He is working to clarify the role of Rho family GTPases in brain function using a newly developed double vector system, combining the retrograde gene transfer vector with adeno-associated viral vector.
Kazuto Kobayashi received a PhD in biochemistry from Nagoya University, Japan, and took a post-doc position in Nagoya University School of Medicine, which was supported by the Japan Society for the Promotion of Science. He worked as an Assistant Professor at the Department of Biochemistry in Nagoya University School of Medicine and at the Division of Neurochemistry at the Fujita Health University School of Medicine. He then moved as an Associate Professor to the Division of Molecular Animal Technology at the Nara Institute of Science and Technology and is currently a Professor of Molecular Genetics at the Fukushima Medical University. He is also a Guest Professor at the National Institute of Physiological Sciences, Okazaki, Japan. His laboratory aims to develop new technologies for genetic manipulation of neural circuits and to elucidate the neural mechanism underlying the acquisition, performance, and recovery of instrumental learning.
Abstract
The major issue in neuroscience is to delineate the structure and function of the neural circuits that mediate a variety of brain functions. One useful approach to resolve this issue is the genetic manipulation of the activity of specific neural circuits, which enables the study of the behavioral and physiological consequences of the manipulation. A novel type of lentiviral vector showing highly efficient retrograde gene transfer (HiRet) can introduce a transgene into neurons that innervate a certain brain region at the vector injection site and confer a fundamental tool for genetically manipulating specific neural pathways. Here, we describe the strategy for the selective targeting of neural pathways by using this HiRet vector, combined with immunotoxin (IT)-mediated cell targeting, which eliminates particular neuronal types genetically engineered to express a receptor for the recombinant IT. Our strategy provides a powerful technology to investigate the framework underlying brain functions and to develop animal models for the dissection of neurological and neuropsychiatric diseases.
About the authors
Shigeki Kato completed a BSc and PhD (Agr.) at the Laboratory of Molecular Biology, Graduate School of Agricultural Science in Tohoku University. Then he took a postdoctoral position in Fukushima Medical University with Professor Kazuto Kobayashi, and is now an Assistant Professor. In 2012, he was awarded a PhD (Med.) at Fukushima Medical University. His principal research interests include how the neural circuit is controlled to conduct a variety of behaviors using genetic manipulation of neural circuit with the retrograde gene transfer vector.
Kenta Kobayashi graduated from Department of Agricultural Chemistry in Kyoto University, and received a PhD in bioscience from Nara Institute of Science and Technology. He worked as Assistant Professor in Fukushima Medical University, and is an currently Associate Professor at the National Institute of Physiological Sciences, Okazaki, Japan. He is working to clarify the role of Rho family GTPases in brain function using a newly developed double vector system, combining the retrograde gene transfer vector with adeno-associated viral vector.
Kazuto Kobayashi received a PhD in biochemistry from Nagoya University, Japan, and took a post-doc position in Nagoya University School of Medicine, which was supported by the Japan Society for the Promotion of Science. He worked as an Assistant Professor at the Department of Biochemistry in Nagoya University School of Medicine and at the Division of Neurochemistry at the Fujita Health University School of Medicine. He then moved as an Associate Professor to the Division of Molecular Animal Technology at the Nara Institute of Science and Technology and is currently a Professor of Molecular Genetics at the Fukushima Medical University. He is also a Guest Professor at the National Institute of Physiological Sciences, Okazaki, Japan. His laboratory aims to develop new technologies for genetic manipulation of neural circuits and to elucidate the neural mechanism underlying the acquisition, performance, and recovery of instrumental learning.
©2013 by Walter de Gruyter Berlin Boston
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Artikel in diesem Heft
- Masthead
- Masthead
- Dissecting circuit mechanisms by genetic manipulation of specific neural pathways
- The basal ganglia: motor and cognitive relationships in a clinical neurobehavioral context
- Putting an object in context and acting on it: neural mechanisms of goal-directed response to contextual object
- Aversive motivation and the maintenance of monogamous pair bonding
- The roles of MAGE-D1 in the neuronal functions and pathology of the central nervous system
- Multiple sclerosis and glutamate excitotoxicity
- Spatial frequencies and emotional perception
- A review on the effectiveness of repetitive transcranial magnetic stimulation (rTMS) on post-stroke aphasia
