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Preparation of [64Cu]Cu–NOTA complex as a potential renal PET imaging agent using 64Cu produced via the direct activation route

  • Sourav Patra , Sachin Jadhav , Priyalata Shetty , Khajan Singh , Ardhi Rajeswari , K. V. Vimalnath , Avik Chakraborty , Rubel Chakravarty and Sudipta Chakraborty ORCID logo EMAIL logo
Published/Copyright: February 14, 2024
Radiochimica Acta
From the journal Radiochimica Acta Volume 112 Issue 7-8

Abstract

Glomerular filtration rate (GFR) could be determined more accurately using renal positron emission tomography (PET) than conventional gamma imaging. Copper-64 [T½ = 12.7 h, Eβ+ (max) = 653 keV, β+ branching ratio = 17.8 %, 1346 keV γ-photon (0.54 %), EC (43.8 %), β− emission (38.4 %)] in the form of its hydrophilic complex with 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) is proposed as a potent formulation for renal PET imaging. A lyophilized kit was developed for formulation of ∼370 MBq dose of [64Cu]Cu–NOTA complex in a facile single step process using 64Cu produced by thermal neutron activation in a research reactor. The complex could be synthesized with >99 % yield and retained its integrity even when challenged by apoferritin. The rapid accumulation of [64Cu]Cu–NOTA in the kidney and clearance through urinary path was demonstrated using PET/CT imaging and ex vivo biodistribution study carried out in healthy Wistar rats to elucidate its effectiveness as a renal PET-imaging agent.

Keywords: glomerular filtration rate; carrier-added 64Cu; NOTA; apoferritin; PET
Corresponding author: Sudipta Chakraborty, Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India; and Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, India, E-mail:

Funding source: Bhabha Atomic Research Centre

Award Identifier / Grant number: Unassigned

Acknowledgments

We would like to acknowledge Dr. S. Kannan, Former Director, Radiochemistry and Isotope Group (RC & IG), Bhabha Atomic Research Centre (BARC), Dr. P.K Mohapatra, Associate Director, RC & IG and Dr. Tapas Das, Head, Radiopharmaceuticals Division, BARC for their valuable support to this work.

  1. Research ethics: All animal experiments were performed as per the protocol approved by the Institutional Animal Ethics Committee of the Bhabha Atomic Research Centre.

  2. Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: The authors state no conflict of interest.

  4. Research funding: None declared.

  5. Data availability: Not applicable.

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Received: 2023-07-21
Accepted: 2023-11-02
Published Online: 2024-02-14
Published in Print: 2024-08-27

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/ract-2023-0206
Keywords for this article
glomerular filtration rate; carrier-added 64Cu; NOTA; apoferritin; PET

Articles in the same Issue

  1. Frontmatter
  2. Preface
  3. NUCAR-2023: Foreword
  4. Research Articles
  5. Theoretical analysis of light and heavy-ion induced reactions: production of medically relevant 97Ru
  6. Excitation functions of alpha-particle induced nuclear reactions on nat Sn
  7. Non-destructive assay of plutonium in absence of gamma-ray spectrometry
  8. Catalytic destruction of oxalate in the supernatant stream generated during plutonium reconversion process
  9. Quantification of Zr in simulated dissolver solution of U–Zr fuel by laser-induced breakdown spectroscopy
  10. Radiochemical and chemical characterization of fuel, salt, and deposit from the electrorefining of irradiated U-6 wt% Zr in hot cells
  11. Zirconium sponge production: an integrated approach for chemical characterization of process intermediates using ICP-OES
  12. Determination of 10B/11B in boric acid and B4C using LA-ICPMS
  13. Evaluating sustainability of Bhuj aquifer system, Western India using nuclear dating techniques
  14. Nanocrystalline Ce(OH)4-based materials: ruthenium selective adsorbent for highly alkaline radioactive liquid waste
  15. Production and radiochemical separation of 68Ge from irradiated Ga–Ni alloy target in 30 MeV cyclotron
  16. Preparation of [64Cu]Cu–NOTA complex as a potential renal PET imaging agent using 64Cu produced via the direct activation route
  17. Total chemical synthesis of PSMA-617: an API for prostate cancer endotherapeutic applications
  18. Rapid screening technique for gross α and gross β estimations in aqueous samples during radiation emergency
  19. Development of Dy3+ doped lithium magnesium borate glass system for thermoluminescence based neutron dosimetry applications
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