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Gel filtration chromatography analysis and modeling the process of pullulan depolymerization

  • Ivan M. Savic EMAIL logo , Goran S. Nikolic , Stanko A. Zerajic and Ivana M. Savic
Published/Copyright: August 25, 2012
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Abstract

Different fractions of pullulan oligosaccharide can be applied in the synthesis of pharmaceutical active compounds. Thus, the object of this study was to model the depolymerization process of pullulan polysaccharide with the change of pH, temperature and depolymerization time. The samples were analyzed by gel filtration chromatography (GFC). The chromatography separation of polymer fractions was achieved by using a Zorbax PSM-300 HP-SEC column (250×6.2 mm, 5 μm) at a temperature of 25°C. The flow rate of the mobile phase (redistilled water) was 1 cm3 min-1. The fractions were recorded by a refractive index detector. The obtained data were converted by thin plate spline interpolation function into matrixes. By the mathematical processing matrixes, the models of molar mass distribution in the function of pH, temperature and depolymerization time were obtained. The intervals of depolymerization parameters can be defined for obtaining the desirable pullulan oligomers, which have the efficient applications in macromolecule technology.


Corresponding author: Ivan M. Savic, Faculty of Technology, University of Nis, Bulevar oslobodjenja 124, 16000 Leskovac, Serbia

Published Online: 2012-08-25
Published in Print: 2012-08-01

©2012 by Walter de Gruyter Berlin Boston

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