Abstract
Objective: The results of a feasibility study of the application of PEG-300 and fructose as two independent optical clearing agents for the reduction of light scattering in biological tissues are presented.
Materials and methods: An OCT system operating at 1300 nm was used to study optical clearing effects. In in-vitro experiments in mice (n=2) an increase of the imaging depth was observed after intravenous injection of PEG-300 alone and in combination with intradermal injection of fructose. The optical clearing effect was also studied for the first time in two mice in vivo using intravenous injection of PEG-300 or solution of hemoglobin.
Results: The intradermal injection of fructose in combination with the intravenous injection of PEG-300 led to a rapid optical clearing effect. In the experiments on mice in vivo the injection of PEG-300 or hemoglobin solution into the tail vein of the living mice allowed for a rapid enhancement of the vein wall and the surrounding tissue image contrast.
Conclusion: The experiments on mice have clearly demonstrated that intradermal and intravenous injections of optical clearing agents enhanced light transport through the skin and blood vessels.
Zusammenfassung
Ziel: Es werden die Ergebnisse einer Machbarkeitsstudie zur Anwendung von PEG-300 und Fructose als zwei unabhängige optische Klärungsmittel zur Verminderung der Lichtstreuung in biologischen Geweben dargestellt.
Material und Methoden: Um die optischen Clearing-Effekte zu studieren, wurde ein OCT-System verwendet, welches bei einer Wellenlänge von 1300 nm betrieben wurde. In In-vitro-Experimenten an zwei Mäusen wurde eine Erhöhung der Abbildungstiefe nach intravenöser Injektion von PEG-300 allein und in Kombination mit einer intradermalen Injektion von Fructose beobachtet. Zudem wurde der optische Clearing-Effekt erstmals in vivo an zwei weiteren Mäusen nach intravenöser Injektion von PEG-300 oder einer Hämoglobinlösung untersucht.
Ergebnisse: Die intradermale Injektion von Fructose in Kombination mit der intravenösen Injektion von PEG-300 führte zu einem schnellen optischen Clearing-Effekt. In den In-vivo-Versuchen konnte nach der Injektion von PEG-300 bzw. der Hämoglobinlösung in die Schwanzvene der lebenden Mäuse eine schnelle Verbesserung des Bildkontrastes der Venenwand sowie des umgebenden Gewebes erreicht werden.
Fazit: Die Experimente an Mäusen haben deutlich gezeigt, dass die intradermale und intravenöse Injektionen von optischen Klärungsmitteln den Lichttransport durch die Haut und Blutgefäße verbessert.
Acknowledgments:
This study was supported by NBIP Ireland funded under the Higher Education Authority PRTLI Cycle 4, co-funded by the Irish Government and the EU Regional Development Fund “Investing in your future”, the Government of the Russian Federation (Grant number: ‘14.Z50.31.0004’) to support scientific research projects implemented under the supervision of leading scientists, and The Tomsk State University Academic D. I. Mendeleev Fund Program. The authors are grateful to Charles McHale (REMEDI, NUI Galway, Ireland) for the technical assistance in the experiments with mice.
Conflict of interest statement
Conflict of interest statement: The authors state no conflict of interest. All authors have read the journal’s Publication Ethics and Publication Malpractice Statement available at the journal’s website and hereby confirm that they comply with all its parts applicable to the present scientific work.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Editorial
- From optical bioimaging to clinical biophotonics
- Magazine section
- Snapshots
- Review
- Photodynamic therapy for cancer of the pancreas – The story so far
- Original contributions
- Fluorescence imaging for photodynamic therapy of non-melanoma skin malignancies – A retrospective clinical study
- Hydrogen peroxide detection in viable and apoptotic tumor cells under action of cisplatin and bleomycin
- Light enhancement of in vitro antitumor activity of galactosylated phthalocyanines
- Investigation on cavitation bubble dynamics induced by clinically available Ho:YAG lasers
- Preliminary research reports
- Enhancement of OCT imaging by blood optical clearing in vessels – A feasibility study
- Effect of temperature regime and compression in OCT imaging of skin in vivo
- Congress announcements
- Congresses 2016
Articles in the same Issue
- Frontmatter
- Editorial
- From optical bioimaging to clinical biophotonics
- Magazine section
- Snapshots
- Review
- Photodynamic therapy for cancer of the pancreas – The story so far
- Original contributions
- Fluorescence imaging for photodynamic therapy of non-melanoma skin malignancies – A retrospective clinical study
- Hydrogen peroxide detection in viable and apoptotic tumor cells under action of cisplatin and bleomycin
- Light enhancement of in vitro antitumor activity of galactosylated phthalocyanines
- Investigation on cavitation bubble dynamics induced by clinically available Ho:YAG lasers
- Preliminary research reports
- Enhancement of OCT imaging by blood optical clearing in vessels – A feasibility study
- Effect of temperature regime and compression in OCT imaging of skin in vivo
- Congress announcements
- Congresses 2016