Abstract
Objective: To compare perforation characteristics of standard 22 G (0.7 mm) to 29 G needle (0.34 mm) for amniocentesis.
Methods: Seventeen human chorio-amnion membranes were perforated immediately after cesarean section using 22 G needle for spinal anesthesia and 29 G “pencil-point” needles for amniocentesis under in-vitro conditions. Area of perforation was determined using a microscope and volume of fluid leakage was measured over a period of 5 min.
Results: Membrane perforation with the 22 G needle resulted in a mean damaged area of 225,147.4 μm2, a hole with a mean area of 50,154 μm2 and amniotic fluid volume passage of 17.5 mL/5 min, whereas the 29 G needle generated a mean damaged area of 114,812.4 μm2, a hole with an average area of 1382.5 μm2 and volume passage of 0.28 mL/5 min. These differences were significant.
Conclusion: The hole formed by membrane perforation with 29 G “pencil-point” needle for amniocentesis is 36 times smaller, and the amniotic fluid loss is 61 times less than that measured with the 22 G standard needle for spinal anesthesia. Significant reduction of complications following amniocentesis is expected with the 29 G needle.
©2011 by Walter de Gruyter Berlin Boston
Articles in the same Issue
- Review articles
- Bridging global gene expression candidates in first trimester placentas with susceptibility loci from linkage studies of preeclampsia
- Candidates for the determination of antithrombin activity in pregnant women
- Opinion paper
- Using prophylactic, but not tocolytic, magnesium sulfate to reduce cerebral palsy related to prematurity: what dose, and what about infant mortality?
- Original articles – Obstetrics
- Vaginal breech delivery in very low birth weight (VLBW) neonates: experience of a single center
- Fetal outcome for infants in breech by method of delivery: experiences with a stand-by service system of senior obstetricians and women's choices of mode of delivery
- Characterization of discriminatory urinary proteomic biomarkers for severe preeclampsia using SELDI-TOF mass spectrometry
- Oxytocin versus dinoprostone vaginal insert for induction of labor after previous cesarean section: a retrospective comparative study
- Mid-trimester amniotic fluid concentrations of the proinflammatory cytokines IL-6, IL-8, TNF-α, and lipopolysaccharide binding protein in normal pregnancies: a prospective evaluation according to parity, gestational age, and fetal gender
- Expression of laminin receptor 1 in human placentas from normal and preeclamptic pregnancies and its relationship with the severity of preeclampsia
- Colonization, serotypes and transmission rates of group B streptococci in pregnant women and their infants born at a single University Center in Germany
- The placental factor in spontaneous preterm labor with and without premature rupture of membranes
- A 29-gauge atraumatic needle for amniocentesis
- Gestational diabetes mellitus complicating twin pregnancies
- Original articles – Fetus
- Results of and further prevention of hypoxic fetal brain damage by inhibition of xanthine oxidase enzyme with allopurinol
- Diastolic forward flow in the fetal main pulmonary artery and its implication for fetal cardiac cycle evaluation
- Abnormalities of the penis in utero – hypospadias on fetal MRI
- Intrapartum cardiotocography (CTG) and ST-analysis of labor in diabetic patients
- Original articles – Newborn
- Ductus venosus flow at 11–13 weeks in the prediction of birth weight discordance in monochorionic twins
- Stability of thyroid hormones during continuous infusion
- Short communication
- Supracervical cerclage with intracavitary balloon to control bleeding associated with placenta previa
- Letter to the Editor
- Two cases of Lowe syndrome presenting as increased fetal nuchal translucency
- Congress calendar
- Congress Calendar
Articles in the same Issue
- Review articles
- Bridging global gene expression candidates in first trimester placentas with susceptibility loci from linkage studies of preeclampsia
- Candidates for the determination of antithrombin activity in pregnant women
- Opinion paper
- Using prophylactic, but not tocolytic, magnesium sulfate to reduce cerebral palsy related to prematurity: what dose, and what about infant mortality?
- Original articles – Obstetrics
- Vaginal breech delivery in very low birth weight (VLBW) neonates: experience of a single center
- Fetal outcome for infants in breech by method of delivery: experiences with a stand-by service system of senior obstetricians and women's choices of mode of delivery
- Characterization of discriminatory urinary proteomic biomarkers for severe preeclampsia using SELDI-TOF mass spectrometry
- Oxytocin versus dinoprostone vaginal insert for induction of labor after previous cesarean section: a retrospective comparative study
- Mid-trimester amniotic fluid concentrations of the proinflammatory cytokines IL-6, IL-8, TNF-α, and lipopolysaccharide binding protein in normal pregnancies: a prospective evaluation according to parity, gestational age, and fetal gender
- Expression of laminin receptor 1 in human placentas from normal and preeclamptic pregnancies and its relationship with the severity of preeclampsia
- Colonization, serotypes and transmission rates of group B streptococci in pregnant women and their infants born at a single University Center in Germany
- The placental factor in spontaneous preterm labor with and without premature rupture of membranes
- A 29-gauge atraumatic needle for amniocentesis
- Gestational diabetes mellitus complicating twin pregnancies
- Original articles – Fetus
- Results of and further prevention of hypoxic fetal brain damage by inhibition of xanthine oxidase enzyme with allopurinol
- Diastolic forward flow in the fetal main pulmonary artery and its implication for fetal cardiac cycle evaluation
- Abnormalities of the penis in utero – hypospadias on fetal MRI
- Intrapartum cardiotocography (CTG) and ST-analysis of labor in diabetic patients
- Original articles – Newborn
- Ductus venosus flow at 11–13 weeks in the prediction of birth weight discordance in monochorionic twins
- Stability of thyroid hormones during continuous infusion
- Short communication
- Supracervical cerclage with intracavitary balloon to control bleeding associated with placenta previa
- Letter to the Editor
- Two cases of Lowe syndrome presenting as increased fetal nuchal translucency
- Congress calendar
- Congress Calendar