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Identification and isolation of putative stem cells from the murine placenta

  • Christine L. Proudfit , Michael K. Chan , Ross S. Basch und Bruce K. Young EMAIL logo
Veröffentlicht/Copyright: 13. September 2014
Journal of Perinatal Medicine
Aus der Zeitschrift Journal of Perinatal Medicine Band 42 Heft 6

Abstract

Objective: The placenta of mid-gestation mice is a known rich source of hematopoietic stem cells. We hypothesized that it is also a source of other multipotent stem cells.

Methods: We isolated fetal cells from the murine placenta across the second half of gestation and characterized their expression of surface antigens known to be associated with mesenchymal stem cells (MSCs) on a subset of hematopoietic lineage-negative cells. Using real-time reverse-transcriptase quantitative polymerase chain reaction, we also evaluated the expression of intracellular transcription factors (TFs) known to be associated with renal development and/or multipotent stem cells.

Results: Cell phenotypes with surface marker and TF expression consistent with multipotent stem cells of a mesenchymal lineage as well as renal cell progenitors were found in the placenta. The expression of MSC and renal progenitor surface markers varied throughout gestation, but was highest on E12–15 where such cells represented a small but significant percentage of the population. Of the studied TFs, 10 of 11 renal TFs were found at moderate to high levels, and all stem cell TFs were found.

Conclusion: The mid-gestation murine placenta may serve as a source of multipotent stem cells and also contains cells which may be renal cell progenitors.

Keywords: Cell progenitors; murine placenta; stem cells
Corresponding author: Bruce K. Young, Department of Obstetrics and Gynecology, New York University Langone Medical Center, New York, NY, USA, Tel.: +1-212-263-6359, Fax: +1-212-263-6329, E-mail:

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The authors stated that there are no conflicts of interest regarding the publication of this article.

Received: 2014-4-23
Accepted: 2014-7-23
Published Online: 2014-9-13
Published in Print: 2014-11-1

©2014 by De Gruyter

Artikel in diesem Heft

  1. Frontmatter
  2. Guest editorial
  3. Guest editorial
  4. Original articles
  5. Identification and isolation of putative stem cells from the murine placenta
  6. Target versus actual oxygenation index at initiation of inhaled nitric oxide in neonates with hypoxic respiratory failure: survey results from 128 patient cases
  7. Omega-3 polyunsaturated fatty acids enhance cytokine production and oxidative stress in a mouse model of preterm labor
  8. Adrenocorticotropic hormone and cortisol levels in term infants born with meconium-stained amniotic fluid
  9. Measurement of novel biomarkers of neuronal injury and cerebral oxygenation after routine vaginal delivery versus cesarean section in term infants
  10. The time-associated impact of the Newborn Influenza Protection Act on infant influenza rates in New York State
  11. Delivery-related knowledge of mothers of NICU infants compared with well-baby-nursery infants
  12. Review article
  13. Mercury exposure in pregnancy: a review
  14. Original articles - Obstetrics
  15. A “multi-hit” model of neonatal white matter injury: cumulative contributions of chronic placental inflammation, acute fetal inflammation and postnatal inflammatory events
  16. Balloon tamponade for the management of postpartum uterine hemorrhage
  17. The IgG avidity value for the prediction of congenital cytomegalovirus infection in a prospective cohort study
  18. Obstetric anal sphincter injury risk reduction: a retrospective observational analysis
  19. Adverse perinatal outcomes related to the delivery mode in women with monochorionic diamniotic twin pregnancies
  20. Letter to the Editor
  21. The factors associated with the failure of transcatheter pelvic arterial embolization for intractable postpartum hemorrhage
  22. Congress Calendar
  23. Congress Calendar
https://doi.org/10.1515/jpm-2014-0266
Schlagwörter für diesen Artikel
Cell progenitors; murine placenta; stem cells

Artikel in diesem Heft

  1. Frontmatter
  2. Guest editorial
  3. Guest editorial
  4. Original articles
  5. Identification and isolation of putative stem cells from the murine placenta
  6. Target versus actual oxygenation index at initiation of inhaled nitric oxide in neonates with hypoxic respiratory failure: survey results from 128 patient cases
  7. Omega-3 polyunsaturated fatty acids enhance cytokine production and oxidative stress in a mouse model of preterm labor
  8. Adrenocorticotropic hormone and cortisol levels in term infants born with meconium-stained amniotic fluid
  9. Measurement of novel biomarkers of neuronal injury and cerebral oxygenation after routine vaginal delivery versus cesarean section in term infants
  10. The time-associated impact of the Newborn Influenza Protection Act on infant influenza rates in New York State
  11. Delivery-related knowledge of mothers of NICU infants compared with well-baby-nursery infants
  12. Review article
  13. Mercury exposure in pregnancy: a review
  14. Original articles - Obstetrics
  15. A “multi-hit” model of neonatal white matter injury: cumulative contributions of chronic placental inflammation, acute fetal inflammation and postnatal inflammatory events
  16. Balloon tamponade for the management of postpartum uterine hemorrhage
  17. The IgG avidity value for the prediction of congenital cytomegalovirus infection in a prospective cohort study
  18. Obstetric anal sphincter injury risk reduction: a retrospective observational analysis
  19. Adverse perinatal outcomes related to the delivery mode in women with monochorionic diamniotic twin pregnancies
  20. Letter to the Editor
  21. The factors associated with the failure of transcatheter pelvic arterial embolization for intractable postpartum hemorrhage
  22. Congress Calendar
  23. Congress Calendar
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