Startseite Genetic low nephron number hypertension is associated with altered expression of osteopontin and CD44 during nephrogenesis*
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Genetic low nephron number hypertension is associated with altered expression of osteopontin and CD44 during nephrogenesis*

  • Anne Freese EMAIL logo , Markus Wehland , Florian Freese , Christian Bamberg , Reinhold Kreutz und Lars Rothermund
Veröffentlicht/Copyright: 15. Dezember 2012

Abstract

Aims: The study set out to investigate whether the osteopontin (OPN)-CD44-integrin-receptor-system is differently regulated during nephrogenesis in inborn nephron deficit, a major determinant of human primary hypertension and cardiovascular disease in adult life.

Methods: We compared a genetic rat model with an inherited nephron deficit, the Munich-Wistar-Froemter rat (MWF), to normotensive Wistar rats during nephrogenesis at day 19 of fetal development (E19) and at postpartal day 7 (D7).

Results: Renal OPN mRNA (–75%, P<0.05) and protein expression (–38%, P<0.05) were strongly decreased at E19 in MWF compared to Wistar. Renal mRNA-expression of CD44 was increased at E19 in MWF (+271%, P<0.05). At D7, renal OPN protein expression was increased (+115%, P<0.05) and renal mRNA-expression of CD44 remained elevated compared to Wistar control (+127%, P<0.05).

Conclusions: Altered fetal expression of the OPN-CD44-integrin-receptor-system in the MWF model points to a possible role in low nephron number hypertension and cardiovascular disease.


Corresponding author: Anne Freese, Dr. med., Klinik für Geburtsmedizin, Charité-Universitätsmedizin Berlin, Campus Virchow-Klinikum, Augustenburgerplatz 1, 13353 Berlin, Germany, Tel.: +49 30 450664594, Fax: +49 30 450564903

The authors stated that there are no conflicts of interest regarding the publication of this article.

  1. *

    Supported by the Deutsche Forschungsgemeinschaft: (DFG, Grant RO 2124/2-1 and RO 2124/2-2).

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Received: 2012-6-31
Accepted: 2012-11-6
Published Online: 2012-12-15
Published in Print: 2013-05-01

©2013 by Walter de Gruyter Berlin Boston

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