PPAR-γ2 Pro12Ala polymorphism is associated with post-challenge abnormalities of glucose homeostasis in children and adolescents with obesity
-
Agnes Jermendy
,
Anna Körner
Abstract
Aim: The aim of the study was to investigate the association between PPAR-γ2 Pro12Ala polymorphism and laboratory characteristics of carbohydrate metabolism in children and adolescents with obesity. In addition, serum levels of tumor necrosis factor (TNF)-α, and soluble form of its receptors (sTNFR1 and sTNFR2) were assessed.
Methods: In a cross-sectional study, 79 obese children and adolescents of Caucasian origin were investigated. PPAR-γ2 Pro12Ala polymorphism was determined using polymerase chain reaction – restriction fragment length polymorphism technique. Serum levels of TNF-α, sTNFR1 and sTNFR2 were measured by enzyme amplified sensitivity immunoassay.
Results: The minor Ala allele frequency was found to be 14.56% in our cohort. No significant differences in age, BMI, waist circumference, blood pressure, serum lipid, uric acid, TNF-α, sTNFR1 and sTNFR2 values were found between carriers of the Ala allele (Pro/Ala and Ala/Ala; n=21) vs. homozygous carriers of the Pro allele (Pro/Pro; n=58). However, post-challenge (120 min) plasma glucose and insulin values were significantly lower in Ala allele carriers vs. homozygous Pro allele carriers (6.56±0.26 vs. 7.36±0.25 mmol/L and 65.9±13.8 vs. 111.8±20.7 μU/mL, respectively; p<0.05); while no significant differences were found at fasting state.
Conclusions: The association between PPAR-γ2 Pro12Ala polymorphism and glucose metabolism is already present in children and adolescents with obesity who might be at the very beginning of the natural course of type 2 diabetes. At this stage, higher insulin sensitivity can be detected in Ala allele carriers compared to homozygous Pro subjects at post-challenge but not in fasting state; however, the TNF-system seems not to be involved in the alteration of glucose homeostasis due to PPAR-γ2 Pro12Ala polymorphism.
©2011 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Publisher's Note
- Publisher's Note
- Editorial
- Sex steroid priming for growth hormone (GH) provocative tests: an endless debate with insufficient solution
- Not ready for prime time
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- Sex hormone priming
- Pros of priming in the diagnosis of growth hormone deficiency
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