Abstract
Background
Eucalyptus oil (EO), derived from Eucalyptus species, possesses vast remedial and healing properties, although its gut health-promoting properties have not been well investigated. In this study, we investigated the chemical composition of a commercial EO formulation and its potential role in protecting against aflatoxin B1 (AfB1)-induced gastrointestinal damage in rats.
Methods
Male Wistar rats were divided into six groups with eight rats each. Control rats were administered with the vehicle (1% Tween 80) for 14 days, while another group was exposed to two oral doses of AFB1 on days 12 and 14. Two other groups were pre-treated with oral doses of EO (50 and 100 mg/kg b.w.) for 14 consecutive days, along with two oral doses of AfB1 (5 mg/kg b.w.) on days 12 and 14. The remaining two groups were treated with EO alone at the two doses for 14 days. At the end of the experiment, blood samples, stomach and intestinal tissues were collected for measurement of oxidative stress and antioxidant parameters and light microscopic examination.
Results
Gas chromatography-mass spectrometry analysis revealed Eucalyptol (1, 8-cineole) as the main constituent (67.48%) of the oil. AfB1 administration induced oxidative and inflammatory disturbances, indicated by significantly (p<0.05) increased serum nitric oxide level and myeloperoxidase activity; increased tissue contents of hydrogen peroxide, malondialdehyde and protein carbonyls, accompanied with corresponding histological alterations. AfB1 also induced significant (p<0.05) reductions in glutathione peroxidase and superoxide dismutase (SOD) activities. Treatment with EO produced significant improvements in the biochemical parameters as well as the appearance of the gastric and intestinal mucosa. EO alone, at the two doses tested did not produce any significant changes in the parameters investigated.
Conclusion
The findings from this study showed that EO demonstrated protective activity against Aflatoxin-induced toxicity in stomach and intestinal tissues and may thus find application in treatment of gastrointestinal disorders.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Ethical statement: All applicable guidelines for the care and use of animals were followed and the research work approved by the University of Ibadan Animal Care and Use Research Ethics Committee (Ref. No UI-ACUREC/17/0026).
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Artikel in diesem Heft
- Review
- Phytochemistry and neuroprotective effects of Eclipta alba (L.) Hassk
- The effects of Vitex agnus-castus on menstrual bleeding: A systematic review and meta-analysis
- RA-Experimental
- Evaluation of the hypoglycemic effect of seven wild folkloric edible plants from Palestine
- Antioxidant activity, carotenoids, chlorophylls and mineral composition from leaves of Pallenis spinosa: an Algerian medicinal plant
- Terminalia ferdinandiana, a traditional medicinal plant of Australia, alleviates hydrogen peroxide induced oxidative stress and inflammation, in vitro
- In vivo neuroprotective, antinociceptive, anti-inflammatory potential in Swiss albino mice and in vitro antioxidant and clot lysis activities of fractionated Holigarna longifolia Roxb. bark extract
- Virgin coconut oil supplementation in diet modulates immunity mediated through survival signaling pathways in rats
- Amelioration of Aflatoxin B1-induced gastrointestinal injuries by Eucalyptus oil in rats
- Effect of crude Moringa oleifera Lam. seed extract on the blood markers of metabolic syndrome in high-fructose diet-fed growing Sprague-Dawley rats
- Warionia saharae induces antihypertensive and vasorelaxant activities through nitric oxide and KATP channels pathways in rats
- Celecoxib, ibuprofen, and indomethacin alleviate depression-like behavior induced by interferon-alfa in mice
- RA-Clinical
- Effect of hot arm and foot bath on heart rate variability and blood pressure in healthy volunteers
- Effectiveness of training program combining chakrayoga and meditation
- Effects of treadmill training on the balance, functional capacity and quality of life in Parkinson’s disease: A randomized clinical trial
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- The effect of successful low-dose immunotherapy ascertained by provocation neutralization on lymphocytic calcium ion influx following electric field exposure
- Effect of emotional regulation training on depression, anxiety and stress among the patients with alcohol dependence admitted in selected de-addiction centers at south India
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- Efficacy of osteopathic treatment in patients with stable moderate-to-severe chronic obstructive pulmonary disease: a randomized controlled pilot study
- Workshop and package education intervention to knowledge, attitude and practice of ICU nurses on the use of physical restraints