In vitro protective effects of Scutellaria litwinowii root extract against H2O2-induced DNA damage and cytotoxicity
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Sousan Shojaee
Abstract
Background: Despite the wide range of investigations on the therapeutic potentials of herbal extracts in cancer cell lines, there is not usually enough data on their probable effects on normal cells. Recently, both anti-tumoral and anti-oxidant properties of Scutellaria litwinowii extracts have been reported on different cancer cell lines. In the present study, the possible protective effects of S. litwinowii root extracts against cytotoxicity and DNA damage induced by hydrogen peroxide (H2O2) on normal cells were investigated using MTT and Comet assays, respectively.
Methods: A method of sequential extraction with solvents of different polarities was used to generate methanolic, de-fatted, and dichloromethane fractions. Both MTT and Comet assays were performed here to assess, respectively, changes in cell viability and levels of DNA damage from H2O2. In a pre-treatment regimen, test materials were applied to the cells prior to H2O2 treatment, while in a co-treatment protocol, cells were treated simultaneously with H2O2 and extracts.
Results: In the co-treatment studies, only methanolic extract at 1,000 μg/mL (p<0.001) imparted a significant protective effect as shown in the MTT assay. Pre-treatment of cells for 24 h with different concentrations of the test materials did not lead to any significant protection. Results from the Comet assays in the co-treatment studies suggested a significant (p<0.01) protective effect of the test materials against DNA damage caused by H2O2. However, in the pre-treatment studies, only methanolic extract at ≥500 μg/mL showed a protective effect (p<0.01).
Conclusions: Considering the probable high levels of phenolic and flavonoid compounds in the methanolic extract, these compounds may impart the noted protective effects of the S. litwinowii root through the scavenging of free radicals.
Acknowledgments
This work was supported financially by a research grant from the Vice Chancellor for Research of Mashhad University of Medical Sciences, Mashhad, Iran. The results described in this paper were part of the S. Shojaee’s PharmD thesis.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Research funding: Vice Chancellor for Research, Mashhad University of Medical Sciences.
Employment or leadership: None declared.
Honorarium: None declared.
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©2014 by Walter de Gruyter Berlin / Boston
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Artikel in diesem Heft
- Frontmatter
- Review
- A review on Ipomoea carnea: pharmacology, toxicology and phytochemistry
- Preclinical Studies
- Biochemical and histopathological study of the anti-hyperglycemic and anti-hyperlipidemic effects of cornelian cherry (Cornus mas L.) in alloxan-induced diabetic rats
- Pharmacognostical standardization and antidiabetic activity of Syzygium cumini (Linn.) barks (Myrtaceae) on streptozotocin-induced diabetic rats
- Preventive effects of the methanol soluble fraction of Millettia macrophylla Benth (Fabaceae) on an osteoporosis-like model of ovariectomized Wistar rats
- Detoxification of Echis ocellatus venom-induced toxicity by Annona senegalensis Pers
- Subacute antidiabetic and in vivo antioxidant effects of methanolic extract of Bridelia micrantha (Hochst Baill) leaf on alloxan-induced hyperglycaemic rats
- Effect of Persea americana (avocado) fruit extract on the level of expression of adiponectin and PPAR-γ in rats subjected to experimental hyperlipidemia and obesity
- In vitro protective effects of Scutellaria litwinowii root extract against H2O2-induced DNA damage and cytotoxicity
- Clinical Studies
- The antiplaque effect of herbal mouthwash in comparison to chlorhexidine in human gingival disease: a randomized placebo controlled clinical trial
- Use of complementary and alternative medical therapies (CAM) by patients attending a regional comprehensive cancer care centre
- Education Forum
- Implementing blended learning into the academic programs of Rajiv Gandhi University of Health Sciences, Karnataka
