Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice
-
Moses Z. Zaruwa
,
Aranya Manosroi
Abstract
The hypoglycemic activities of nine sub-fractions from the methanolic leaf crude extract of Anisopus mannii were investigated in normoglycemic and alloxan-induced diabetic mice. The methanolic sub-fraction M at 400 mg/kg bw showed significantly (p<0.05) high reduction in fasting blood glucose (FBG) at 27.36 and 65.57% in normoglycemic and diabetic mice, respectively. In acute toxicity test, M at 2,000 and 5,000 mg/kg bw showed reduction in blood urea nitrogen and creatinine level, elevations in aspartate transaminase, alanine transaminase and total bilirubin levels, as well as the body weights. The weight-ratios of kidney and liver to the body weight of the mice fed with these doses of M were reduced with no sign of histopathological alteration. The M at 250 mg/kg bw significantly reduced the FBG levels in a postprandial study. The hypoglycemic effect of M was eliminated when co-administered with isosorbide dinitrate or nifedipine indicating the induction of insulin secretion via K+ ATP-dependent channels. The UV/HPLC analysis of M indicated saponin at 7.7 mg/g. This study confirmed the traditional use of A. mannii for diabetes mellitus and the potential for the further development as a novel hypoglycemic drug.
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©2013 by Walter de Gruyter Berlin / Boston
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Articles in the same Issue
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- Masthead
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- Hepatoprotective activity of Calotropis gigantea flowers against carbon-tetrachloride-induced liver damage in mice
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- Antimalarial potential of China 30 and Chelidonium 30 in combination therapy against lethal rodent malaria parasite: Plasmodium berghei
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- Cytotoxic effects of crocin on MOLT-4 human leukemia cells
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- Tea toxicity and cholinesterase inhibition of Huilliche herbal medicine
- Iatrogenic venous thrombosis secondary to supplemental medicine toxicity
- Clinical Studies
- Decreasing cardiovascular risk factors in obese individuals using a combination of PGX® meal replacements and PGX® granules in a 12-week clinical weight modification program
- The use of an anti-inflammatory supplement in patients with chronic kidney disease
- Impact of relaxation training according to the Yoga In Daily Life® system on anxiety after breast cancer surgery
- Positive impact of Tai Chi Chuan participation on biopsychosocial quality of life compared to exercise and sedentary controls: a cross-sectional survey
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