A tablet derived from Andrographis paniculata complements dihydroartemisinin-piperaquine treatment of malaria in pregnant mice

Bastiana; Aty Widyawaruyanti; Hilkatul Ilmi; Lidya Tumewu; Budi Prasetyo; Achmad Fuad Hafid; Aryati

doi:10.1515/jbcpp-2020-0162

Article

A tablet derived from Andrographis paniculata complements dihydroartemisinin-piperaquine treatment of malaria in pregnant mice

Bastiana , Aty Widyawaruyanti , Hilkatul Ilmi , Lidya Tumewu , Budi Prasetyo , Achmad Fuad Hafid and Aryati

Published/Copyright: February 11, 2021

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 33 Issue 2

Abstract

Objectives

The use of standard antimalarial drugs, such as dihydroartemisinin-piperaquine (DHP) for the treatment of malaria during pregnancy is limited due to the risk of teratogenicity. The alternative is therefore required although few exist. Here we show a phytopharmaceutical drug derived from Andrographis paniculata (AS201-01), which is effective as herbal antimalarial both in vitro and in vivo and may be a suitable alternative when used in complementary treatment with DHP.

Methods

Plasmodium berghei infected pregnant BALB/c mice were divided into four groups: G1 (negative control), G2 (AS201-01), G3 (DHP), and G4 (combination of DHP and AS201-01). Pheripheral blood was collected during therapy for counting parasitemia. Placental samples were analyzed for the expression of IFN-γ, TNF- α, IL-10, placental parasite counts and foetal morphology.

Results

Groups G4 and G3 both showed a 100% inhibition of peripheral parasitemia. However, the treatment in G4 was found to be less effective than that in G2 and G3 in preventing placental parasitemia. The G4 treatment was able to reduce the expression of IFN-γ and IL-10, whereas TNF-α was not significantly different from the control group. Foetal morphologic abnormalities were observed in all groups except G2; G4 showed lower percentage of abnormalities compared to G3 and G1.

Conclusions

A combination of A. paniculata tablet (AS201-01) with DHP has the potential to reduce the toxicity of DHP in malaria treatment.

Keywords: Andrographis paniculata ; dihydroartemisinin-piperaquine; foetal morphology; immune system; placental malaria

Corresponding author: Aty Widyawaruyanti, Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, C Campus Universitas Airlangga, Mulyorejo, Surabaya 60115, Indonesia; and Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, C Campus Universitas Airlangga, Mulyorejo, Surabaya 60115, Indonesia, Phone: +628113404171, E-mail: aty-w@ff.unair.ac.id

Funding source: Universitas Airlangga

Award Identifier / Grant number: 564/UN3.14/LT/2016

Acknowledgments

The authors thank the management of Institute of Tropical Disease of Universitas Airlangga for providing support in terms of facilities.

Research funding: This research was funded by Airlangga University through Mandat Research Grant 2016 contract no. 564/UN3.14/LT/2016.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: This experimental study has received ethical approval from the Ethics Committee of the Faculty of Veterinary Medicine, Universitas Airlangga No: 2.KE.185.10.2019.

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Received: 2020-05-25

Accepted: 2020-11-04

Published Online: 2021-02-11

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2020-0162

Keywords for this article

Andrographis paniculata; dihydroartemisinin-piperaquine; foetal morphology; immune system; placental malaria