Piperine protects oxidative modifications in human erythrocytes

Prabhakar Singh; Kanti Bhooshan Pandey; Syed Ibrahim Rizvi

doi:10.1515/jbcpp-2020-0100

Article

Piperine protects oxidative modifications in human erythrocytes

Prabhakar Singh , Kanti Bhooshan Pandey and Syed Ibrahim Rizvi

Published/Copyright: February 8, 2021

Published by

Become an author with De Gruyter Brill

Submit Manuscript Author Information Explore this Subject

From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 33 Issue 2

Abstract

Objectives

Piperine (1-piperoyl piperidine), a major alkaloid constituent of Piper nigrum L. and Piper longum L. has pleiotropic biological effects, but the mechanism(s) involved remain to be elucidated. The current study was conducted to examine the efficacy of antioxidant ability of piperine on t-BHP induced markers of oxidative stress in human erythrocytes.

Methods

Healthy human erythrocytes and erythrocytes membrane was stressed with free radical inducer chemical; t-BHP (10⁻⁵ M), and the effects of piperine was measured against free radical mediated modification in lipid and protein content, –SH and GSH value with antioxidant potential.

Results

The results demonstrate that treatment of erythrocytes with piperine (10⁻⁵ to 10⁻⁷ M) significantly (p<0.05) ameliorated the adverse consequences of oxidative stress as evidenced by prevention of oxidation of erythrocyte reduced glutathione, membrane thiols, proteins, and peroxidation of lipids; the effects were in correlation with ferric reducing and radical scavenging abilities of piperine.

Conclusions

The study concludes that piperine possesses potent anti-oxidant potential which may explain many of its observed biological effects.

Keywords: antioxidant; biomarkers; oxidative stress; piperine; protein carbonyls

Corresponding author: Syed Ibrahim Rizvi, Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, 211002, India, Phone: +91-9415305910, E-mail: sirizvi@gmail.com

Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: All volunteers were informed and had given their consent for participation in the study. The study protocol was in conformity with the rule and regulation of the Allahabad University Ethical Committee. The study complies with the World Medical Association Declaration of Helsinki regarding ethical conduct of research.

References

1. Srinivasan, K. Black pepper and its pungent principle-piperine: a review of diverse physiological effects. Crit Rev Food Sci Nutr 2007;47:735–48. https://doi.org/10.1080/10408390601062054.Search in Google Scholar

2. Butt, MS, Pasha, I, Sultan, MT, Randhawa, MA, Saeed, F, Ahmed, W. Black pepper and health claims: a comprehensive treatise. Crit Rev Food Sci Nutr 2013;53:875–86. https://doi.org/10.1080/10408398.2011.571799.Search in Google Scholar

3. Halliwell, B, Gutteridge, JMC. Cellular responses to oxidative stress: adaptation, damage, repair, senescence and death. In: Halliwell, B, Gutteridge, JMC, editors. Free radical biology and medicine, 4th ed. New York: Oxford University Press; 2007.Search in Google Scholar

4. Pandey, KB, Rizvi, SI. Biomarkers of oxidative stress in red blood cells. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2011;155:131–6. https://doi.org/10.5507/bp.2011.027.Search in Google Scholar

5. Pandey, KB, Rizvi, SI. Role of resveratrol in regulation of membrane transporters and integrity of human erythrocytes. Biochem Biophys Res Commun 2014;453:521–6. https://doi.org/10.1016/j.bbrc.2014.09.117.Search in Google Scholar

6. Madhavi, BB, Bhavana, M, Nath, AR, Prasad, M, Vennela, KS. In vitro evaluation of piperine enclosed erythrocyte carriers. Drug Invent Today 2013;5:169–74. https://doi.org/10.1016/j.dit.2013.04.006.Search in Google Scholar

7. Pandey, KB, Rizvi, SI. Protective effect of resveratrol on markers of oxidative stress in human erythrocytes subjected to in vitro oxidative insult. Phytother Res 2010;24:S11–14. https://doi.org/10.1002/ptr.2853.Search in Google Scholar

8. Esterbauer, H, Cheeseman, KH. Determination of aldehydic lipid peroxidation products: malondialdehyde and 4-hydroxynonenal. Methods Enzymol 1990;186:407–13. https://doi.org/10.1016/0076-6879(90)86134-h.Search in Google Scholar

9. Pandey, KB, Mishra, N, Rizvi, SI. Protective Role of myricetin on markers of oxidative stress in human erythrocytes subjected to oxidative stress. Nat Prod Commun 2009;4:221–6. https://doi.org/10.1177/1934578x0900400211.Search in Google Scholar

10. Kitajima, H, Amaguchi, T, Kinoto, E. Hemolysis of human erythrocytes under hydrostatic pressure is suppressed by cross-linking of membrane proteins. J Biochem 1990;108:1057–62. https://doi.org/10.1093/oxfordjournals.jbchem.a123305.Search in Google Scholar PubMed

11. Benzie, IFF, Strain, JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996;239:70–6. https://doi.org/10.1006/abio.1996.0292.Search in Google Scholar PubMed

12. Pandey, KB, Rizvi, SI. Ferric reducing and radical scavenging activities of selected important polyphenols present in foods. Int J Food Prop 2012;15:702–8. https://doi.org/10.1080/10942912.2010.498547.Search in Google Scholar

13. Kato, Y, Mori, Y, Makino, Y, Morimitsu, Y, Hiroi, S, Ishikawa, T, et al.. Formation of NE-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 1999;274:20406–14. https://doi.org/10.1074/jbc.274.29.20406.Search in Google Scholar

14. Meister, A. Glutathione deficiency produced by inhibition of its synthesis, and its reversal; Applications in research and therapy. Pharmacol Ther 1991;51:155–94. https://doi.org/10.1016/0163-7258(91)90076-x.Search in Google Scholar

15. Hawkins, CL, Morgan, PE, Davies, MJ. Quantification of protein modification by oxidants. Free Radic Biol Med 2009;46:965–88. https://doi.org/10.1016/j.freeradbiomed.2009.01.007.Search in Google Scholar

16. Khajuria, A, Thusu, N, Zutshi, N. Piperine modulates permeability characteristics of intestine by inducing alterations in membrane dynamics: influence on brush border membrane fluidity, ultrastructure and enzyme kinetics. Phytomedicine 2002;9:224–31. https://doi.org/10.1078/0944-7113-00114.Search in Google Scholar

17. Umar, S, Golam Sarwar, AH, Umar, K, Ahmad, N, et al.. Piperine ameliorates oxidative stress, inflammation and histological outcome in collagen induced arthritis. Cell Immunol 2013;284:51–9. https://doi.org/10.1016/j.cellimm.2013.07.004.Search in Google Scholar

18. Naidu, KA, Thippeswamy, NB. Inhibition of human low density lipoprotein oxidation by active principles from spices. Mol Cell Biochem 2002;229:19–23.10.1023/A:1017930708099Search in Google Scholar

19. Vijayakumar, RS, Surya, D, Nalini, N. Antioxidant efficacy of black pepper (Piper nigrum L.) and piperine in rat with in high fat diet induced oxidative stress. Redox Rep 2004;49:105–10. https://doi.org/10.1179/135100004225004742.Search in Google Scholar

20. Rauscher, FM, Sanders, RA, Watkins, JB. Effects of piperine on antioxidant pathways in tissues from normal and streptozotocin-induced diabetic rats. J Biochem Mol Toxicol 2000;14:329–34. https://doi.org/10.1002/1099-0461(2000)14:6<329::aid-jbt5>3.0.co;2-g.10.1002/1099-0461(2000)14:6<329::AID-JBT5>3.0.CO;2-GSearch in Google Scholar

21. Abdel-Daim, MM, Sayed, AA, Abdeen, A, Aleya, L, Ali, D, Alkahtane, AA, et al.. Piperine enhances the antioxidant and anti-inflammatory activities of thymoquinone against microcystin-LR-induced hepatotoxicity and neurotoxicity in mice. Oxid Med Cell Longev 2019;2019:1309175. https://doi.org/10.1155/2019/1309175.Search in Google Scholar

22. Pandey, KB, Rizvi, SI. Recent advances in health promoting effect of dietary polyphenols. Curr Nutr Food Sci 2012;8:254–64.10.2174/157340112803832228Search in Google Scholar

23. Tiwari, A, Mahadik, KR, Gabhe, SY. Piperine: a comprehensive review of methods of isolation, purification, and biological properties. Med Drug Discov 2020;7:1–21. https://doi.org/10.1016/j.medidd.2020.100027.Search in Google Scholar

24. Zadorozhna, M, Tataranni, T, Mangieri, D. Piperine: role in prevention and progression of cancer. Mol Biol Rep 2019;46:5617–29. https://doi.org/10.1007/s11033-019-04927-z.Search in Google Scholar PubMed

25. Chuchawankul, S, Khorana, N, Poovorawan, Y. Piperine inhibits cytokine production by human peripheral blood mononuclear cells. Genet Mol Res 2012;11:617–27. https://doi.org/10.4238/2012.march.14.5.Search in Google Scholar

26. Kakarala, M, Dubey, SK, Tarnowski, M, Cheng, C, Liyanage, S, Strawder, T, et al.. Ultra-low flow liquid chromatography assay with ultraviolet (UV) detection for piperine quantitation in human plasma. J Agric Food Chem 2010;58:6594–9. https://doi.org/10.1021/jf100657r.Search in Google Scholar PubMed PubMed Central

Received: 2020-05-04

Accepted: 2020-10-31

Published Online: 2021-02-08

You are currently not able to access this content.

Articles in the same Issue

https://doi.org/10.1515/jbcpp-2020-0100

Keywords for this article

antioxidant; biomarkers; oxidative stress; piperine; protein carbonyls