Startseite Strain specific response of mice to IMQ-induced psoriasis
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Strain specific response of mice to IMQ-induced psoriasis

  • Murali Badanthadka , Lidwin D’Souza und Fathima Salwa
Veröffentlicht/Copyright: 8. Februar 2021
Journal of Basic and Clinical Physiology and Pharmacology
Aus der Zeitschrift Journal of Basic and Clinical Physiology and Pharmacology Band 32 Heft 5

Abstract

Objectives

Psoriasis is an autoimmune, inflammatory disease that needs a reliable animal model. Imiquimod (IMQ)-induced psoriasis is a widely used preclinical tool for psoriasis research. However, this model is sensitive to the genetic variation of mice. The present study explores mice’s genetic background on disease stability and severity induced by IMQ.

Methods

Three distinct strains of mice (Balb/c, C57BL/6, and Swiss albino) were divided into four groups (Vaseline, IMQ, IMQ+Clobetasol, and IMQ+Curcumin). Psoriasis area severity index (PASI) score, ear/back skin thickness, body weight alterations, and histopathological examination were employed to analyze disease severity. The spleen index studied the systemic effect. Strain effect on oxidative stress induced by IMQ was evaluated by estimating antioxidant factors, superoxide dismutase (SOD), catalase, and glutathione (GSH).

Results

IMQ application resulted in increased PASI score, thickness, and alterations in body weight, confirming disease development in all the mice. However, the disease stability/severity between these strains was not identical. Although IMQ application caused splenomegaly, IMQ+curcumin treated C57BL/6 mice demonstrated a synergistic effect of IMQ and curcumin on the spleen resulting in increased splenomegaly. Decreased cellular enzyme activity in SOD, Catalase, and levels of GSH was observed in IMQ challenged mice, indicating the participation of the redox system in the genesis of the disease that was comparable among the strains.

Conclusions

These results indicate the existence of strain-dependent development of the disease. The Swiss model was found to be better in terms of disease severity and stability than other models. Further, a detailed mechanistic study might help to explain the pathological difference between these strains.

Keywords: clobetasol; curcumin; imiquimod; mice strain; strain-effect
Corresponding author: Murali Badanthadka, Department of Nitte University Centre for Animal Research and Experimentation (NUCARE), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), NITTE (Deemed to be University), Paneer, Deralakatte, Mangalore, 575 018, India, Phone +91 0824 2203991, E-mail:

Acknowledgments

The authors would like to thank Dr. Hrishikesh Damle, MD & CEO, Atrimed Pharmaceuticals Pvt Ltd, Bangalore for providing Curcumin gel for our study. We also thank Dr. Kishan Prasad H. L., Additional professor of pathology, KSHEMA, Deralakatte, Mangalore – 575018 for histopathology slide reading.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The experimental protocols were approved by Institutional Animal Ethics Committee (IAEC), and all experiments were carried out following Committee for the Purpose of Control and Supervision of Experiment on Animals (CPCSEA) guidelines.

References

1. Bocheńska, K, Smolińska, E, Moskot, M, Jakóbkiewicz-Banecka, J, Gabig-Cimińska, M. Models in the research process of psoriasis. Int J Mol Sci 2017;18:2514.10.3390/ijms18122514Suche in Google Scholar

2. Hawkes, JE, Adalsteinsson, JA, Gudjonsson, JE, Ward, NL. Research techniques made simple: murine models of human psoriasis. J Invest Dermatol 2018;138:e1–8. https://doi.org/10.1016/j.jid.2017.10.013.Suche in Google Scholar

3. Murphy, S, Ellis-Hutchings, R, Finch, L, Welz, S, Wiench, K. Critical evaluation of 2-ethylhexyl acrylate dermal carcinogenicity studies using contemporary criteria. Toxicol Lett 2018;294:205–11. https://doi.org/10.1016/j.toxlet.2018.05.016.Suche in Google Scholar

4. Surcel, M, Huică, RI, Munteanu, AN, Isvoranu, G, Pîrvu, IR, Ciotaru, D, et al.. Phenotypic changes of lymphocyte populations in psoriasiform dermatitis animal model. Exp Ther Med 2019;17:1030–8. https://doi.org/10.3892/etm.2018.6978.Suche in Google Scholar

5. Coleman, DL. Obese and diabetes: two mutant genes causing diabetes-obesity syndromes in mice. Diabetologia 1978;14:141–8. https://doi.org/10.1007/bf00429772.Suche in Google Scholar

6. Beckwith, J, Cong, Y, Sundberg, JP, Elson, CO, Leiter, EH. Cdcs1, a major colitogenic locus in mice, regulates innate and adaptive immune response to enteric bacterial antigens. Gastroenterology 2005;129:1473–84. https://doi.org/10.1053/j.gastro.2005.07.057.Suche in Google Scholar

7. Radulovic, J, Kammermeier, J, Spiess, J. Generalization of fear responses in C57BL/6N mice subjected to one-trial foreground contextual fear conditioning. Behav Brain Res 1998;95:179–89. https://doi.org/10.1016/s0166-4328(98)00039-4.Suche in Google Scholar

8. Roth, DM, Swaney, JS, Dalton, ND, Gilpin, EA, Ross, JJr. Impact of anesthesia on cardiac function during echocardiography in mice. Am J Physiol Heart Circ Physiol 2002;282:H2134–40. https://doi.org/10.1152/ajpheart.00845.2001.Suche in Google Scholar PubMed

9. van der Fits, L, Mourits, S, Voerman, JS, Kant, M, Boon, L, Laman, JD, et al.. Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23/IL-17 axis. J Immunol 2009;182:5836–45. https://doi.org/10.4049/jimmunol.0802999.Suche in Google Scholar PubMed

10. Surcel, M, Munteanu, AN, Huică, RI, Isvoranu, G, Pîrvu, IR, Constantin, C, et al.. Reinforcing involvement of NK cells in psoriasiform dermatitis animal model. Exp Ther Med 2019;18:4956–66. https://doi.org/10.3892/etm.2019.7967.Suche in Google Scholar PubMed PubMed Central

11. Stanisavljević, S, Đedović, N, Vujičić, M, Saksida, T, Jevtić, B, Momčilović, B, et al.. Strain-specific helper T cell profile in the gut-associated lymphoid tissue. Immunol Lett 2017;190:282–8.10.1016/j.imlet.2017.08.017Suche in Google Scholar PubMed

12. Walter, A, Schäfer, M, Cecconi, V, Matter, C, Urosevic-Maiwald, M, Belloni, B, et al.. Aldara activates TLR7-independent immune defence. Nat Commun 2013;4:1560. https://doi.org/10.1038/ncomms2566.Suche in Google Scholar PubMed

13. Swindell, WR, Michaels, KA, Sutter, AJ, Diaconu, D, Fritz, Y, Xing, X, et al.. Imiquimod has strain-dependent effects in mice and does not uniquely model human psoriasis. Genome Med 2017;9:24. https://doi.org/10.1186/s13073-017-0415-3.Suche in Google Scholar PubMed PubMed Central

14. Bezdek, S, Hdnah, A, Sezin, T, Mousavi, S, Zillikens, D, Ibrahim, S, et al.. The genetic difference between C57Bl/6J and C57Bl/6N mice significantly impacts Aldara™-induced psoriasiform dermatitis. Exp Dermatol 2017;26:349–51. https://doi.org/10.1111/exd.13131.Suche in Google Scholar PubMed

15. Mekada, K, Abe, K, Murakami, A, Nakamura, S, Nakata, H, Moriwaki, K, et al.. Genetic differences among C57BL/6 substrains. Exp Anim 2009;58:141–9. https://doi.org/10.1538/expanim.58.141.Suche in Google Scholar PubMed

16. Goldstein, JM, Wagers, AJ. What’s in a (Sub)strain? Stem Cell Rep 2018;11:303–5. https://doi.org/10.1016/j.stemcr.2018.07.011.Suche in Google Scholar PubMed PubMed Central

17. Luo, DQ, Wu, HH, Zhao, YK, Liu, JH, Wang, F. Original research: different imiquimod creams resulting in differential effects for imiquimod-induced psoriatic mouse models. Exp Biol Med 2016;241:1733–8. https://doi.org/10.1177/1535370216647183.Suche in Google Scholar PubMed PubMed Central

18. Alvarez, P, Jensen, LE. Imiquimod treatment causes systemic disease in mice resembling generalized pustularpsoriasis in an IL-1 and IL-36 dependent manner. Mediat Inflamm 2016;2016:6756138. https://doi.org/10.1155/2016/6756138.Suche in Google Scholar PubMed PubMed Central

19. Sun, J, Dou, W, Zhao, Y, Hu, J. A comparison of the effects of topical treatment of calcipotriol, camptothecin, clobetasol and tazarotene on an imiquimod-induced psoriasis-like mouse model. Immunopharmacol Immunotoxicol 2013;36:17–24. https://doi.org/10.3109/08923973.2013.862542.Suche in Google Scholar PubMed

20. Sun, J, Zhao, Y, Hu, J. Curcumin inhibits imiquimod-induced psoriasis-like inflammation by inhibiting IL-1beta and IL-6 production in mice. PLoS One 2013;8:e67078. https://doi.org/10.1371/journal.pone.0067078.Suche in Google Scholar PubMed PubMed Central

21. Wan, T, Pan, W, Long, Y, Yu, K, Liu, S, Ruan, W, et al.. Effects of nanoparticles with hydrotropic nicotinamide on tacrolimus: permeability through psoriatic skin and antipsoriatic and antiproliferative activities. Int J Nanomed 2017;12:1485–97. https://doi.org/10.2147/ijn.s126210.Suche in Google Scholar

22. Sun, J, Zhao, Y, Jin, H, Hu, J. Curcumin relieves TPA-induced Th1 inflammation in K14-VEGF transgenic mice. Int Immunopharm 2015;25:235–41. https://doi.org/10.1016/j.intimp.2015.02.007.Suche in Google Scholar

23. Kadam, DP, Suryakar, AN, Ankush, RD, Kadam, CY, Deshpande, KH. Role of oxidative stress in various stages of psoriasis. Indian J Clin Biochem 2010;25:388–92. https://doi.org/10.1007/s12291-010-0043-9.Suche in Google Scholar

24. Baek, JO, Byamba, D, Wu, WH, Kim, TG, Lee, MG. Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress. Arch Dermatol Res 2012;304:699–706. https://doi.org/10.1007/s00403-012-1272-y.Suche in Google Scholar

25. Baker, BS, Brent, L, Valdimarsson, H, Powles, AV, al-Imara, L, Walker, M, et al.. Is epidermal cell proliferation in psoriatic skin grafts on nude mice driven by T-cell derived cytokines? Br J Dermatol 1992;126:105–10. https://doi.org/10.1111/j.1365-2133.1992.tb07805.x.Suche in Google Scholar

26. Ellman, GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70–7. https://doi.org/10.1016/0003-9861(59)90090-6.Suche in Google Scholar

27. Kakkar, P, Das, B, Vishwanathan, PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130–32.Suche in Google Scholar

28. Aebi, H. Catalase in vitro. Methods Enzymol 1984;105:121–6. https://doi.org/10.1016/s0076-6879(84)05016-3.Suche in Google Scholar

29. Lowry, OH, Rosebrough, NJ, Farr, AL, Randall, RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75. https://doi.org/10.1016/s0021-9258(19)52451-6.Suche in Google Scholar

30. Boehncke, WH, Schön, MP. Animal models of psoriasis. Clin Dermatol 2007;25:596–605. https://doi.org/10.1016/j.clindermatol.2007.08.014.Suche in Google Scholar PubMed

31. D’Souza, L, Badanthadka, M, Salwa, F. Effect of animal strain on model stability to imiquimod-induced psoriasis. Indian J Physiol Pharmacol 2020;64:83–91.Suche in Google Scholar

32. Salwa, F, Badanthadka, M, D’Souza, L. Differential psoriatic effect of imiquimod on BALB/c and Swiss mice. J Health Allied Sci NU [Under revision].Suche in Google Scholar

33. Badanthadka, M, D’Souza, L. Imiquimod Induced psoriasis mice model: a promising tool for psoriasis research? Res J Pharm Technol 2020;13:3508–15. https://doi.org/10.5958/0974-360x.2020.00621.6.Suche in Google Scholar

34. Arijit, C, Meghana, P, Shyam, V, Koushik, L. Side-effects of topical steroids: a long overdue revisit. Indian Dermatol Online J 2014;5:416–25.10.4103/2229-5178.142483Suche in Google Scholar PubMed PubMed Central

35. Sarafian, G, Afshar, M, Mansouri, P, Asgarpanah, J, Raoufinejad, K, Rajabi, M. Topical turmeric microemulgel in the management of plaque psoriasis; a clinical evaluation. Iran J Pharm Res 2015;14:865–76.Suche in Google Scholar

36. Steiniger, BS. Human spleen microanatomy: why mice do not suffice. Immunology 2015;145:334–46. https://doi.org/10.1111/imm.12469.Suche in Google Scholar PubMed PubMed Central

37. Krummen, MB, Varga, G, Steinert, M, Stuetz, A, Luger, TA, Grabbe, S. Effect of pimecrolimus vs. corticosteroids on murine bone marrow-derived dendritic cell differentiation, maturation and function. Exp Dermatol 2006;15:43–50. https://doi.org/10.1111/j.0906-6705.2005.00395.x.Suche in Google Scholar PubMed

38. Jariwala, SP. The role of dendritic cells in the immunopathogenesis of psoriasis. Arch Dermatol Res 2007;299:359–66. https://doi.org/10.1007/s00403-007-0775-4.Suche in Google Scholar PubMed PubMed Central

39. Li, X, Liu, X. Effect of curcumin on immune function of mice. J Huazhong Univ Sci Technol - Med Sci 2005;25:137–40.10.1007/BF02873559Suche in Google Scholar PubMed

40. Li, J, Niu, R, Dong, L, Gao, L, Zhang, J, Zheng, Y, et al.. Nanoencapsulation of curcumin and its protective effects against CCl4-induced hepatotoxicity in mice. J Nanomater 2019;2019:1–10. https://doi.org/10.1155/2019/7140132.Suche in Google Scholar

41. Jiao, Y, Wilkinson, J4th, Di, X, Wang, W, Hatcher, H, Kock, ND, et al.. Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator. Blood 2009;113:462–9. https://doi.org/10.1182/blood-2008-05-155952.Suche in Google Scholar PubMed PubMed Central

42. Chin, D, Huebbe, P, Frank, J, Rimbach, G, Pallauf, K. Curcumin may impair iron status when fed to mice for six months. Redox Biol 2014;2:563–9. https://doi.org/10.1016/j.redox.2014.01.018.Suche in Google Scholar PubMed PubMed Central

43. Kimura, M, Tarumoto, Y, Nakane, S, Otomo, S. Comparative toxicity study of hydrocortisone 17-butyrate 21-propionate (HBP) ointment and other topical corticosteroids in rats. Drugs Exp Clin Res 1986;12:643–52.Suche in Google Scholar

44. Zhang, Z, Chen, Y, Xiang, L, Wang, Z, Xiao, GG, Hu, J. Effect of curcumin on the diversity of gut microbiota in ovariectomized rats. Nutrients 2017;9:1146. https://doi.org/10.3390/nu9101146.Suche in Google Scholar PubMed PubMed Central

45. Zhou, Q, Mrowietz, U, Rostami-Yazdi, M. Oxidative stress in the pathogenesis of psoriasis. Free Radic Biol Med 2009;47:891–905. https://doi.org/10.1016/j.freeradbiomed.2009.06.033.Suche in Google Scholar PubMed

46. Birben, E, Sahiner, UM, Sackesen, C, Erzurum, S, Kalayci, O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9–19. https://doi.org/10.1097/wox.0b013e3182439613.Suche in Google Scholar PubMed PubMed Central

47. Lin, X, Huang, T. Oxidative stress in psoriasis and potential therapeutic use of antioxidants. Free Radic Res 2016;50:585–95. https://doi.org/10.3109/10715762.2016.1162301.Suche in Google Scholar PubMed

48. Liu, A, Zhao, W, Zhang, B, Tu, Y, Wang, Q, Li, J. Cimifugin ameliorates imiquimod-induced psoriasis by inhibiting oxidative stress and inflammation via NF-κB/MAPK pathway. Biosci Rep 2020;40:BSR20200471. https://doi.org/10.1042/bsr20200471.Suche in Google Scholar PubMed PubMed Central

49. Rychlik, KA, Secrest, JR, Lau, C, Pulczinski, J, Zamora, ML, Leal, J, et al.. Utero ultrafine particulate matter exposure causes offspring pulmonary immunosuppression. Proc Natl Acad Sci USA 2019;116:3443–8. https://doi.org/10.1073/pnas.1816103116.Suche in Google Scholar PubMed PubMed Central

50. Boysen, G. The glutathione conundrum: stoichiometric disconnect between its formation and oxidative stress. Chem Res Toxicol 2017;30:1113–6. https://doi.org/10.1021/acs.chemrestox.7b00018.Suche in Google Scholar PubMed PubMed Central

51. Zeng, Y, Zhao, H, Zhang, T, Zhang, C, He, Y, Du, L, et al.. Curcumin against imiquimod-induced psoriasis of mice through IL-6/STAT3 signaling pathway. Biosci Rep 2020 Jun 18. https://doi.org/10.1042/BSR20192842 [Epub ahead of print].Suche in Google Scholar PubMed

52. Varma, SR, Sivaprakasam, TO, Mishra, A, Prabhu, S, Rafiq, M, Rangesh, P. Imiquimod-induced psoriasis-like inflammation in differentiated Human keratinocytes: its evaluation using curcumin. Eur J Pharmacol 2017;813:33–41. https://doi.org/10.1016/j.ejphar.2017.07.040.Suche in Google Scholar PubMed

53. Jaques, JA, Rezer, JF, Ruchel, JB, Souza, VD, Pinheiro, KD, Schlemmer, KB, et al.. An experimental model of contact dermatitis: evaluation of the oxidative profile of Wister rats treated with free and nanoencapsulated clobetasol. Redox Rep 2012;17:206–13. https://doi.org/10.1179/1351000212y.0000000024.Suche in Google Scholar
Received: 2020-04-23
Accepted: 2020-10-09
Published Online: 2021-02-08

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. N-acetyl-cysteine as adjuvant therapy in female infertility: a systematic review and meta-analysis
  4. Climate change and declining fertility rate in Malaysia: the possible connexions
  5. Original Articles
  6. Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide
  7. Environmental and climatic impact on the infection and mortality of SARS-CoV-2 in Peru
  8. Prophylactic intravenous paracetamol use in extremely premature infants for patent ductus arteriosus
  9. Evaluation of heart rate variability, blood pressure and lipid profile alterations from dual transceiver mobile phone radiation exposure
  10. Strain specific response of mice to IMQ-induced psoriasis
  11. Curcumin exerts hepatoprotection via overexpression of Paraoxonase-1 and its regulatory genes in rats undergone bile duct ligation
  12. Zinc oxide nanoparticles with green tea extract complex in the pancreas of rats against monosodium glutamate toxicity
  13. Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro
  14. Protective and curative role of vitamin D and hormones on the cadmium-induced inhibition of proliferation of human osteoblast cells
  15. The effect of bee pollen on reproductive and biochemical parameters in methotrexate-induced testicular damage in adult rats
  16. Histomorphometric study on effects of monosodium glutamate in liver tissue of Wistar rats
  17. Diligent profiling of preclinical safety of the silk protein sericin
https://doi.org/10.1515/jbcpp-2020-0112
Schlagwörter für diesen Artikel
clobetasol; curcumin; imiquimod; mice strain; strain-effect

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. N-acetyl-cysteine as adjuvant therapy in female infertility: a systematic review and meta-analysis
  4. Climate change and declining fertility rate in Malaysia: the possible connexions
  5. Original Articles
  6. Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide
  7. Environmental and climatic impact on the infection and mortality of SARS-CoV-2 in Peru
  8. Prophylactic intravenous paracetamol use in extremely premature infants for patent ductus arteriosus
  9. Evaluation of heart rate variability, blood pressure and lipid profile alterations from dual transceiver mobile phone radiation exposure
  10. Strain specific response of mice to IMQ-induced psoriasis
  11. Curcumin exerts hepatoprotection via overexpression of Paraoxonase-1 and its regulatory genes in rats undergone bile duct ligation
  12. Zinc oxide nanoparticles with green tea extract complex in the pancreas of rats against monosodium glutamate toxicity
  13. Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro
  14. Protective and curative role of vitamin D and hormones on the cadmium-induced inhibition of proliferation of human osteoblast cells
  15. The effect of bee pollen on reproductive and biochemical parameters in methotrexate-induced testicular damage in adult rats
  16. Histomorphometric study on effects of monosodium glutamate in liver tissue of Wistar rats
  17. Diligent profiling of preclinical safety of the silk protein sericin
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