Methanol extract of Caesalpinia benthamiana normalizes blood pressure and attenuates oxidative stress in uninephrectomized hypertensive rats
-
Idayat Titilayo Gbadamosi
,
Temitayo Olabisi Ajibade
Abstract
Objectives
Hypertension is the number one risk factor and primary contributor of cardiovascular diseases. Caesalpinia benthamiana is a valuable medicinal plant with unvalidated anti-hypertensive activity. This study was carried out to explore the antihypertensive effect of C. benthamiana on uninephrectomized hypertensive rats.
Methods
Fifty rats were grouped into five groups, each containing 10 animals: Group A-normal control (normotensive); B-uninephrectomized control; C-uninephrectomized treated with 50 mg/kg C. benthamiana extract; D-uninephrectomized treated with 100 mg/kg C. benthamiana; and E- uninephrectomized treated with 10 mg/kg of Lisinopril.
Results
Significant increases were observed in systolic, diastolic and mean blood pressure of uninephrectomized control rats. Furthermore, markers of oxidative stress (malondialdehyde, hydrogen peroxide, protein carbonyl, myeloperoxidase and the advanced oxidative protein products) increased significantly while antioxidant status (reduced glutathione, glutathione peroxidase, glutathione S-transferase and superoxide dismutase), reduced significantly in uninephrectomized hypertensive rats. Histopathology revealed thrombosis and occlusion of coronary vessels in the heart, and congestion in the kidney. However, the observed high blood pressure parameters were remarkably normalized together with reduction in markers of oxidative stress and improvement in antioxidant defence system of uninephrectomized hypertensive rats treated with C. benthamiana extract similar to normotensive rats.
Conclusions
C. benthamiana extract exhibited antihypertensive action, strong antioxidant ability, attenuated oxidative stress-mediated hypertension and lessened the development of cardiac and renal damage associated with hypertension induced by uninephrectomy and high dietary intake of salt. Together, C. benthamiana extract might be useful in the management of hypertension due to volume overload in the cardiovascular system.
Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: The ethical approval was obtained from the Animal Care and Use in Research Ethical Committee (ACUREC), University of Ibadan, Ibadan, Oyo State, Nigeria.
References
1. Lackland, DT, Egan, BM, Fan, ZJ, Syddal, HE. Low birth weight contributes to the excess prevalence of end-stage renal disease in African Americans. J Clin Hypertens 2001;3:29–31. https://doi.org/10.1111/j.1524-6175.2001.00828.x.Suche in Google Scholar
2. Pizzino, G, Irrera, N, Cucinotta, M, Pallio, G, Mannino, F, Arcoraci, V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev 2017;9:3886–907.10.1155/2017/8416763Suche in Google Scholar PubMed PubMed Central
3. Navarro-Yepes, J, Zavala-Flores, L, Anandhan, A, Wang, F, Skotak, M, Chandra, N. Antioxidant gene therapy against neuronal cell. Pharmacol Therapeut 2014;142:206–300. https://doi.org/10.1016/j.pharmthera.2013.12.007.Suche in Google Scholar
4. Mayet, J, Hughes, A. Cardiac and vascular pathophysiology in hypertension. Heart 2003;89:1104–09. https://doi.org/10.1136/heart.89.9.1104.Suche in Google Scholar
5. Ho, E, Karimi, GK, Liu, CC, Bhindi, R, Figtree, GA. Biological markers of oxidative stress: applications to cardiovascular research and practice. Redox Biol 2013;8:483–91. https://doi.org/10.1016/j.redox.2013.07.006.Suche in Google Scholar
6. Lawrentschuk, N, Trottier, G, Mayo, K, Rendon, RA. Effects of partial nephrectomy on postoperative blood pressure. Korean J Urol 2012;53:154–8. https://doi.org/10.4111/kju.2012.53.3.154.Suche in Google Scholar
7. Strazzullo, P, D’Elia, L, Kandala, NB, Cappuccio, FP. Salt intake, stroke, and cardiovascular disease: meta-analysis of prospective studies. Br Med J 2009;339:4567–78. https://doi.org/10.1136/bmj.b4567.Suche in Google Scholar
8. Hattori, T, Murase, T, Sugiura, Y, Nagasawa, K, Takahashi, K, Ohtake, M, et al. Effect of salt status and blockade of mineralocorticoid receptors on aldosterone-induced cardiac injury. Hypertens Res 2014;37:125–33. https://doi.org/10.1038/hr.2013.124.Suche in Google Scholar
9. Van Koppen, A, Verhaar, MC, Bongartz, LG, Joles, JA. 5/6th nephrectomy in combination with high salt diet and nitric oxide synthase inhibition to induce chronic kidney disease in the lewis rat. J Vis Exp 2014;77:50398–403. https://doi.org/10.3791/50398.Suche in Google Scholar
10. Herendeen, PS, Zarucchi, JL. Validation of Caesalpinia subgenus Mezoneuron (Desf.) vidal and new combinations in Caesalpinia for two species from Africa. Ann Missouri Bot Gard 1990;77:854–5. https://doi.org/10.2307/2399679.Suche in Google Scholar
11. Dickson, RA, Houghton, PJ, Hylands, PJ. Antibacterial and antioxidant cassane diterpenoids from Caesalpinia benthamiana. Phytochemistry 2007;68:1436–41. https://doi.org/10.1016/j.phytochem.2007.03.008.Suche in Google Scholar
12. Zamble, A, Martin-Nizard, F, Sahpaz, S, Hennebelle, T, Staels, B, Bordet, R. Vasoactivity, antioxidant and aphrodisiac properties of Caesalpinia benthamiana roots. J Ethnopharmacol 2008;116:112–9. https://doi.org/10.1016/j.jep.2007.11.016.Suche in Google Scholar
13. Farm Animal Welfare Council (FAWC). Five freedoms. London: FAWC; 1979.Suche in Google Scholar
14. PHS (PUBLIC HEALTH SERVICE). Policyon Humane Care and the Use of Laboratory Animals. Washington, DC: US Department of Health and Humane Services; 1996. p. 99–158.Suche in Google Scholar
15. Carlstrom, M, Persson, AE, Larsson, E, Hezel, M, Scheffer, PG, Teerlink, T, et al. Dietary nitrate attenuates oxidative stress, prevents cardiac and renal injuries, and reduces blood pressure in salt-induced hypertension. Cardiovasc Res 2011;89:547–85. https://doi.org/10.1093/cvr/cvq366.Suche in Google Scholar
16. Calderon, A, Barrios, V, Escobar, C, Ferrer, E, Barrios, S, González-Pedel, V. Detection of left ventricular hypertrophy by different electrocardiographic criteria in clinical practice. Findings from the sara study. Clin Exp Hypertens 2010;32:145–53. https://doi.org/10.3109/10641960903254455.Suche in Google Scholar
17. Gornal, AG, Bardawill, JC, David, MM. Determination of serum proteins by means of biuret reaction. J Biol Chem 1949;177:751–66. PMID: 18110453.10.1016/S0021-9258(18)57021-6Suche in Google Scholar
18. Olaleye, SB, Adaramoye, OA, Erigbali, PP, Adeniyi, OS. Lead exposure increases oxidative stress in the gastric mucosa of HCl ethanol exposed rats. World J Gastroenterol 2007;13:5121–6. https://doi.org/10.3748/wjg.v13.i38.5121.Suche in Google Scholar
19. Xia, Y, Zweier, JL. Measurement of myeloperoxidase in leukocyte-containing tissues. Anal Biochem 1997;24:93–6. https://doi.org/10.1006/abio.1996.9940.Suche in Google Scholar
20. Kayali, R, Cakatay, U, Akcay, T, Altug, T. Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat. Cell Biochem Funct 2006;24:79–85. https://doi.org/10.1002/cbf.1190.Suche in Google Scholar
21. Wolff, SP. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol 1994;233:182–9. https://doi.org/10.1016/s0076-6879(94)33021-2.Suche in Google Scholar
22. Misra, H, Fridovich, I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170–5. PMID: 4623845.10.1016/S0021-9258(19)45228-9Suche in Google Scholar
23. Oyagbemi, AA, Omobowale, TO, Asenuga, ER, Ochigbo, GO, Adejumobi, AO, Adedapo, AA, et al. Sodium arsenite-induced cardiovascular and renal dysfunction in rat via oxidative stress and protein kinase B (Akt/PKB) signaling pathway. Redox Rep 2017;22:467–77. https://doi.org/10.1080/13510002.2017.1308910.Suche in Google Scholar
24. Beutler, E, Duron, O, Kelly, BM. Improved method for the determination of blood. J Lab Clin Med 1963;61:882–8. PMID: 13967893.Suche in Google Scholar
25. Ellman, GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70–7. https://doi.org/10.1016/0003-9861(59)90090-6.Suche in Google Scholar
26. Habig, WH, Pabst, MJ, Jacoby, WB. Glutathione S-transferase activity: the enzymic step in mercapturic acid formation. J Biol Chem 1974;249:130–9. PMID: 4436300.10.1016/S0021-9258(19)42084-XSuche in Google Scholar
27. Jacques-Silva, MC, Nogueira, CW, Broch, LC, Flores, EMM, Rocha, JBT. Diphenyl diselenide and ascorbic acid changes deposition of selenium and ascorbic acid in liver and brain of mice. Pharmacol Toxicol 2001;88:119–25. https://doi.org/10.1034/j.1600-0773.2001.d01-92.x.Suche in Google Scholar
28. Reznick, AZ, Packer, L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 1994;233:357–63. https://doi.org/10.1016/s0076-6879(94)33041-7.Suche in Google Scholar
29. Draper, HH, Hadley, M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990;186:421–31. https://doi.org/10.1016/0076-6879(90)86135-i.Suche in Google Scholar
30. Drury, RA, Wallington, EA, Cancerson, R, editors. Carlton’s histopathological techniques. London, New York: Oxford; 1976, vol 4.Suche in Google Scholar
31. Carlstrom, M, Persson, AE, Larsson, E, Hezel, M, Scheffer, PG, Teerlink, T, et al. Dietary nitrate attenuates oxidative stress, prevents cardiac and renal injuries, and reduces blood pressure in salt-induced hypertension. Cardiovasc Res 2011;89:547–85. https://doi.org/10.1093/cvr/cvq366.Suche in Google Scholar
32. Kurakova, L, Misak, A, Tomasova, L, Cacanyiova, S, Berenyiova, A, Ondriasova, E, et al. Mathematical relationships of patterns of 35 rat haemodynamic parameters for conditions of hypertension resulting from decreased nitric oxide bioavailability. Exp Physiol 2020;105:312–34. https://doi.org/10.1113/ep088148.Suche in Google Scholar
33. Leloup, AJA, Van Hove, CE, De Moudt, S, De Keulenaer, GW, Fransen, P. Ex vivo aortic stiffness in mice with different eNOS activity. Am J Physiol Heart Circ Physiol 2020;318. H1233–44. https://doi.org/10.1152/ajpheart.00737.2019.Suche in Google Scholar
34. Förstermann, U, Sessa, WC. Nitric oxide synthases: regulation and function. Eur Heart J 2012;33:829–37. https://doi.org/10.1093/eurheartj/ehr304.Suche in Google Scholar
35. Xiao, L, Harrison, DG. Inflammation in hypertension. Can J Cardiol 2020;36:635–47. https://doi.org/10.1016/j.cjca.2020.01.013.Suche in Google Scholar
36. Touyz, RM, Rios, FJ, Alves-Lopes, R, Neves, KB, Camargo, LL, Montezano, AC. Oxidative stress: a unifying paradigm in hypertension. Can J Cardiol 2020;36:659–70. https://doi.org/10.1016/j.cjca.2020.02.081.Suche in Google Scholar
37. Tinoy, JK, Widlansky, ME. Natural antioxidants and hypertension: promise and challenges. Cardiovasc Ther 2010;28:e20–32. https://doi.org/10.1111/j.1755-5922.2010.00137.x.Suche in Google Scholar
38. Brody, S. A randomized controlled trial of high dose ascorbic acid for reduction of blood pressure, cortisol, and subjective responses to psychological stress. Psychopharmacology 2002;159:319–24. https://doi.org/10.1007/s00213-001-0929-6.Suche in Google Scholar
39. Kaneto, H, Xu, G, Song, KH, Suzuma, K, Bonner-Weir, S, Sharma, A. Activation of the hexosamine pathway leads to deterioration of pancreatic beta-cell function through the induction of oxidative stress. J Biol Chem 2001;276:31099–104. https://doi.org/10.1074/jbc.m104115200.Suche in Google Scholar
40. Rodrigo, R. Prevention of postoperative atrial fibrillation: novel and safe strategy based on the modulation of the antioxidant system. Front Physiol 2012;3:118–29. https://doi.org/10.3389/fphys.2012.00093.Suche in Google Scholar
41. Choi, J, Roche, H, Caquet, T. Characterization of superoxide dismutase activity in Chironomus riparius Mg. (Diptera Chironomidae). Larvae–a potential biomarker. Comp Biochem Physiol C Toxicol Pharmacol 1999;124:73–81. https://doi.org/10.1016/s0742-8413(99)00045-6.Suche in Google Scholar
42. Kar, S, Palit, S, Ball, WB, Das, PK. Carnosic acid modulates Akt/IKK/NF-κB signaling by PP2A and induces intrinsic and extrinsic pathway mediated apoptosis in human prostate carcinoma PC-3 cells. Apoptosis 2012;17:735–47. https://doi.org/10.1007/s10495-012-0715-4.Suche in Google Scholar
43. Mohan, S, Gupta, D. Crosstalk of toll-like receptors signaling and Nrf2 pathway for regulation of inflammation. Biomed Pharmacother 2018;108:1866–78. https://doi.org/10.1016/j.biopha.2018.10.019.Suche in Google Scholar
44. Lin, L, Zhao, GJ, Qin, LL. Association between advanced oxidation protein products (AOPP) and vascular calcification in uremic patients. Eur Rev Med Pharmacol Sci 2017;21:4147–52.Suche in Google Scholar
45. Klima, Ł, Kawecka-Jaszcz, K, Stolarz-Skrzypek, K, Menne, J, Fijorek, K, Olszanecka, A, et al. Structure and function of large arteries in hypertension in relation to oxidative stress markers. Kardiol Pol 2013;71:917–23. https://doi.org/10.5603/kp.2013.0226.Suche in Google Scholar
46. Chiu-Braga, YY, Hayashi, SY, Schafranski, M, Messias-Reason, IJ. Further evidence of inflammation in chronic rheumatic valve disease (CRVD): high levels of advanced oxidation protein products (AOPP) and highly sensitive C-reactive protein (hs-CRP). Int J Cardiol 2006;109:275–6. https://doi.org/10.1016/j.ijcard.2005.04.030.Suche in Google Scholar
47. Alderman, CJ, Shah, S, Foreman, JC, Chain, BM, Katz, DR. The role of advanced oxidation protein products in regulation of dendritic cell function. Free Radic Biol Med 2002;32:377–85. https://doi.org/10.1016/s0891-5849(01)00735-3.Suche in Google Scholar
48. Loria, V, Dato, I, Graziani, F, Biasucci, LM. Myeloperoxidase: a new biomarker of inflammation in ischemic heart disease and acute coronary syndromes. Mediators Inflamm 2008;2008:135625. https://doi.org/10.1155/2008/135625.Suche in Google Scholar
49. Kumar, AP, Ryan, C, Cordy, V, Reynolds, WF. Inducible nitric oxide synthase expression is inhibited by myeloperoxidase. Nitric Oxide 2005;13:42–53. https://doi.org/10.1016/j.niox.2005.04.002.Suche in Google Scholar
50. Hou, FF, Zhang, X, Zhang, GH, Xie, D, Chen, PY, Zhang, WR, et al. Efficacy and safety of benazepril for advanced chronic renal insufficiency. N Engl J Med 2006;354:131–40. https://doi.org/10.1056/nejmoa053107.Suche in Google Scholar
© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Review
- Update of hypothyroidism and its management in Unani medicine
- Original Articles
- 6-OHDA mediated neurotoxicity in SH-SY5Y cellular model of Parkinson disease suppressed by pretreatment with hesperidin through activating L-type calcium channels
- Modulation of sleep using electrical vestibular nerve stimulation prior to sleep onset: a pilot study
- Nitric oxide mediates the antidepressant-like effect of modafinil in mouse forced swimming and tail suspension tests
- Effect of mobile phone usage on cognitive functions, sleep pattern, visuospatial ability in Parkinsons patients; a possible correlation with onset of clinical symptoms
- Food-added azodicarbonamide alters haematogical parameters, antioxidant status and biochemical/histomorphological indices of liver and kidney injury in rats
- Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes
- p53 upregulated mediator of apoptosis (Puma) deficiency increases survival of adult neural stem cells generated physiologically in the hippocampus, but does not protect stem cells generated in surplus after an excitotoxic lesion
- In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats
- Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats
- Amine-modified kaolinite clay preserved thyroid function and renal oxidative balance after sub-acute exposure in rats
- Aqueous extract of dry powder blend of seeds and leaves of Picralima nitida (Stapf) T. & H. Durand reduce pain and inflammation in animal models
- Methanol extract of Caesalpinia benthamiana normalizes blood pressure and attenuates oxidative stress in uninephrectomized hypertensive rats
- Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice
- Correlation between biofilm formation and antimicrobial susceptibility pattern toward extended spectrum β-lactamase (ESBL)- and non-ESBL-producing uropathogenic bacteria
Artikel in diesem Heft
- Frontmatter
- Review
- Update of hypothyroidism and its management in Unani medicine
- Original Articles
- 6-OHDA mediated neurotoxicity in SH-SY5Y cellular model of Parkinson disease suppressed by pretreatment with hesperidin through activating L-type calcium channels
- Modulation of sleep using electrical vestibular nerve stimulation prior to sleep onset: a pilot study
- Nitric oxide mediates the antidepressant-like effect of modafinil in mouse forced swimming and tail suspension tests
- Effect of mobile phone usage on cognitive functions, sleep pattern, visuospatial ability in Parkinsons patients; a possible correlation with onset of clinical symptoms
- Food-added azodicarbonamide alters haematogical parameters, antioxidant status and biochemical/histomorphological indices of liver and kidney injury in rats
- Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes
- p53 upregulated mediator of apoptosis (Puma) deficiency increases survival of adult neural stem cells generated physiologically in the hippocampus, but does not protect stem cells generated in surplus after an excitotoxic lesion
- In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats
- Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats
- Amine-modified kaolinite clay preserved thyroid function and renal oxidative balance after sub-acute exposure in rats
- Aqueous extract of dry powder blend of seeds and leaves of Picralima nitida (Stapf) T. & H. Durand reduce pain and inflammation in animal models
- Methanol extract of Caesalpinia benthamiana normalizes blood pressure and attenuates oxidative stress in uninephrectomized hypertensive rats
- Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice
- Correlation between biofilm formation and antimicrobial susceptibility pattern toward extended spectrum β-lactamase (ESBL)- and non-ESBL-producing uropathogenic bacteria
