Curative effects of the aqueous extract of Tithonia diversifolia (Hemsl.) A. gray (Asteraceae) against ethanol induced-hepatotoxicity in rats
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Ivan Stève Tsopmejio Nguepi
Abstract
Objectives
Tithonia diversifolia (Asteraceae) is used in Cameroonian traditional medicine for the treatment of several diseases amongst which are hepatic disorders. Anti-inflammatory, analgesic and anti-diabetic properties have been reported but, there is no scientific information on its hepato-protective effects. The aim of this study was to evaluate the curative effects of the Tithonia diversifolia (T. diversifolia) leaves aqueous extract on ethanol induced-hepatotoxicity in rats.
Methods
Ethanol 40° (4 g/kg) was administered daily by intragastric gavage for 21 days, and then the extract was administered concomitantly with ethanol for two more weeks. Some biochemical serum and tissue parameters were evaluated. Histopathologic analysis of the liver was carried out.
Results
The ingestion of ethanol induced a significant reduction of body weight and a significant increase in some markers of hepatic function (Alanine Amino-transferase, Aspartate Amino-transferase, alkaline phosphatase, gamma glutamyl-transferase, total bilirubin and albumin). These alterations were accompanied by a significant increase in the levels of serum triglycerides (p<0.001). Intoxicated animals were also characterized by a significant decrease of reduced glutathione and nitrites concentrations, catalase and superoxide dismutase activities as well as an increase of malondialdehyde levels. The histopathological examination showed vascular congestion, disorganized parenchyma, liver inflammation and dilation of sinusoid. The extract at the doses of 60 and 120 mg/kg reversed ethanol-induced adverse effects.
Conclusion
Our study found that, the aqueous extract of T. diversifolia leaves has hepato-protective activity against ethanol-induced liver damages due partly to its antioxidant effect. This result justifies its empirical use for the treatment of liver problems.
Acknowledgment
The authors thank Mr. Fogou for the ethnopharmacological information and the procedure to prepare the extract.
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Research funding: None declared.
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Author contributions: Theophile Dimo designed the study. Tsopmejio Ivan Stéve Nguepi, Tsofack Florence Ngueguim and Adolphe Mbatchou conducted pharmacological and biochemical studies. Tsopmejio Ivan Stève Nguepi, Tsofack Florence Ngueguim and Kamkumo Raceline Gounoue performed histological studies and have been involved in drafting the manuscript. Adolphe Mbatchou and Kamkumo Raceline Gounoue performed statistical analyses. All authors contributed substantially to the manuscript, accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: All procedures in this study followed the principles of laboratory animal use and care of the “European community” guidelines (EEC Directive 2010/63/EEC) and were approved by the “Animal Ethical Committee” of the Faculty of Science, University of Yaoundé I.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Review
- Historical account of endocrinal disorders in Unani medicine
- Original Articles
- Impact of sleep disturbances and autonomic dysfunction on the quality of life of patients with fibromyalgia
- Effects of date seeds administration on anxiety and depressive symptoms in streptozotocin-induced diabetic rats: biochemical and behavioral evidences
- Piroxicam reduces acute and chronic pain response in type 1 diabetic rats
- Protective effect of Elateriospermum tapos in maternal obesity-induced deficit cognitive function of the offspring
- Pharmacological investigations on efficacy of Phlorizin a sodium-glucose co-transporter (SGLT) inhibitor in mouse model of intracerebroventricular streptozotocin induced dementia of AD type
- Concurrent administration of acetaminophen and ethanol: impact on mouse liver and testis
- Electrical vestibular nerve stimulation as an adjunctive therapy in the management of type 2 diabetes
- Effect of roxithromycin on contractile activity of gastrointestinal smooth muscles in colitic rats
- Anti-inflammatory and analgesic effects of methanol extract of red cultivar Allium cepa bulbs in rats and mice
- Prophylactic effect of aquatic extract of stevia on acetic acid induced-ulcerative colitis in male rats: a possible role of Nrf2 and PPARγ
- Molecular docking study of flavonoid compounds for possible matrix metalloproteinase-13 inhibition
- Inhibition of key enzymes linked to snake venom induced local tissue damage by kolaviron
- Hepatoprotective effects of Quassia amara stem bark against cadmium-induced toxicity in male Wistar rats
- Curative effects of the aqueous extract of Tithonia diversifolia (Hemsl.) A. gray (Asteraceae) against ethanol induced-hepatotoxicity in rats
- The effect of deer antler from East Kalimantan to increase trabecular bone density and calcium levels in serum on osteoporotic mice
- Case Report
- A case of statin-induced liver injury with positive rechallenge with a second statin. Is there a class effect?
Artikel in diesem Heft
- Frontmatter
- Review
- Historical account of endocrinal disorders in Unani medicine
- Original Articles
- Impact of sleep disturbances and autonomic dysfunction on the quality of life of patients with fibromyalgia
- Effects of date seeds administration on anxiety and depressive symptoms in streptozotocin-induced diabetic rats: biochemical and behavioral evidences
- Piroxicam reduces acute and chronic pain response in type 1 diabetic rats
- Protective effect of Elateriospermum tapos in maternal obesity-induced deficit cognitive function of the offspring
- Pharmacological investigations on efficacy of Phlorizin a sodium-glucose co-transporter (SGLT) inhibitor in mouse model of intracerebroventricular streptozotocin induced dementia of AD type
- Concurrent administration of acetaminophen and ethanol: impact on mouse liver and testis
- Electrical vestibular nerve stimulation as an adjunctive therapy in the management of type 2 diabetes
- Effect of roxithromycin on contractile activity of gastrointestinal smooth muscles in colitic rats
- Anti-inflammatory and analgesic effects of methanol extract of red cultivar Allium cepa bulbs in rats and mice
- Prophylactic effect of aquatic extract of stevia on acetic acid induced-ulcerative colitis in male rats: a possible role of Nrf2 and PPARγ
- Molecular docking study of flavonoid compounds for possible matrix metalloproteinase-13 inhibition
- Inhibition of key enzymes linked to snake venom induced local tissue damage by kolaviron
- Hepatoprotective effects of Quassia amara stem bark against cadmium-induced toxicity in male Wistar rats
- Curative effects of the aqueous extract of Tithonia diversifolia (Hemsl.) A. gray (Asteraceae) against ethanol induced-hepatotoxicity in rats
- The effect of deer antler from East Kalimantan to increase trabecular bone density and calcium levels in serum on osteoporotic mice
- Case Report
- A case of statin-induced liver injury with positive rechallenge with a second statin. Is there a class effect?
