Anti-inflammatory and analgesic effects of methanol extract of red cultivar Allium cepa bulbs in rats and mice

Adeoye Joshua Oyewusi; Olayinka Ayotunde Oridupa; Adebowale Bernard Saba; Ibironke Kofoworola Oyewusi; Jonny Olufemi Olukunle

doi:10.1515/jbcpp-2020-0080

Article

Anti-inflammatory and analgesic effects of methanol extract of red cultivar Allium cepa bulbs in rats and mice

Adeoye Joshua Oyewusi , Olayinka Ayotunde Oridupa , Adebowale Bernard Saba , Ibironke Kofoworola Oyewusi and Jonny Olufemi Olukunle

Published/Copyright: February 8, 2021

Published by

Become an author with De Gruyter Brill

Submit Manuscript Author Information Explore this Subject

From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 32 Issue 6

Abstract

Objectives

Several cultivars of Allium cepa L. have been studied for anti-inflammatory and analgesic activities but there is inadequate information on such biological activities of the concentrated extracts of the Nigerian grown red cultivar A. cepa bulb.

Methods

The anti-inflammatory models used in this study were Carrageenan-induced paw oedema and formalin-induced paw lick in rats, while acetic acid-induced abdominal writhing, hot plate reaction, hot water tail flick tests in mice were the analgesic models.

Results

At 30 min post-induction (pi), the inhibition of paw oedema (62.50%) by 200 mg/kg of methanol extract of red cultivar A. cepa bulb (MERCACB) was significantly (p<0.001) higher than that of indomethacin (15.63%) at 10 mg/kg. The paw oedema inhibition at 60 min pi by MERCACB (76.92%) was significantly higher than that of indomethacin (41.03%). At the early phase of formalin paw-lick test, the pain reaction time (PRT) of rat treated with MERCACB (400 mg/kg) was significantly lower than that of indomethacin and the control groups. The hotplate test revealed that PRT of mice treated with 800 mg/kg of MERCACB were significantly (p<0.01) longer in comparism to indomethacin and control groups. The PRT of mice subjected to thermal pain due to hot water and treated with 800 mg/kg of MERCACB was significantly (p<0.05) longer than that of the control group.

Conclusions

These findings indicate that MERCACB possesses potent anti-inflammatory and analgesic properties which confirm the traditional use of the plant for the treatment of inflammatory diseases and may be useful as a future therapeutic agent.

Keywords: analgesic; anti-inflammatory; mice; rats; red cultivar Allium cepa L

Corresponding author: Adeoye Joshua Oyewusi, Department of Veterinary Pharmacology and Toxicology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria; and Department of Veterinary Pharmacology and Toxicology, University of Ibadan, Ibadan, Nigeria, Phone: +234 8036676864, E-mail: oyewusija@funaab.edu.ng

Acknowledgements

We want to acknowledge the technical support of the laboratory staff the department of Veterinary Physiology and Pharmacology, Federal University of Agriculture, Abeokuta, Nigeria.

Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: This declaration is made to affirm that this study was carried out with the approval of the “ Animal care, Use and Research Ethics Committee (ACUREC) of the University of Ibadan with approval reference: UI-ACUREC/App/03/2017/014.

References

1. Chandrasoma, P, Taylor, CR. Part A. General Pathology, Section II. The Host Response to Injury, Chapter 3. The Acute Inflammatory Response, sub-section: Cardinal Clinical Signs, Concise Pathology. New York: McGraw-Hill; 2005.Search in Google Scholar

2. Howcroft, TK, Judith, C, Germaine, BL, Martyn, TS, Bradley, W, Tony, W, et al.. The role of inflammation in age‐related disease. Aging 2013;1:84–93. https://doi.org/10.18632/aging.100531.Search in Google Scholar PubMed PubMed Central

3. Nicholson, B. Responsible prescribing of opioids for the management of chronic pain. Drugs 2003;63:17–32. https://doi.org/10.2165/00003495-200363010-00002.Search in Google Scholar PubMed

4. Vellucci, R. Heterogeneity of chronic pain. Clin. Drug Investig 2012;32:3–10. https://doi.org/10.2165/11630030-000000000-00000.Search in Google Scholar PubMed

5. Treede, RD, Jensen, TS, Campbell, JN, Cruccu, G, Dostrovsky, JO, Griffin, JW, et al.. Neuropathic pain: redefinition and a grading system for clinical and research purposes. Neurology 2008;70:1630–5. https://doi.org/10.1212/01.wnl.0000282763.29778.59.Search in Google Scholar PubMed

6. Evers, GCM. Pseudo-opioid-resistant pain. Support Care Cancer 1997;5:457–60. https://doi.org/10.1007/s005200050114.Search in Google Scholar PubMed

7. Morrone, LA, Scuteria, D, Rombolàa, L, Mizoguchic, H, Bagetta, G. Opioids resistance in chronic pain management. Curr Neuropharmacol 2017;15:444–56. https://doi.org/10.2174/1570159x14666161101092822.Search in Google Scholar PubMed PubMed Central

8. Oboh, IE, Onwukaema, DN. Analgesic, anti-inflammatory and anti-ulcer activities of Sida acuta in mice and rat. Niger J Nat Prod Med 2005;9:19–21.10.4314/njnpm.v9i1.11827Search in Google Scholar

9. Hassan, HS, Ahmadu, AA, Hassan, AS. Analgesic and anti-inflammatory activities of Asparagus africanus root extract. Afr J Tradit Complement Altern Med 2008;5:27–31. https://doi.org/10.4314/ajtcam.v5i1.31252.Search in Google Scholar PubMed PubMed Central

10. Awe, SO, Olajide, OA, Adeboye, JO, Makinde, JM. Some pharmacological studies on Morinda lucida. Indian J Pharmacol 1998;30:38–42.Search in Google Scholar

11. Gunawardena, D, Shanmugam, K, Govindaraghavan, S, Low, M, Bennett, L, Head, R, et al.. Determination of anti-inflammatory activities of standardised preparations of plant- and mushroom-based foods. Euro J Nutr 2014;53:335–43. https://doi.org/10.1007/s00394-013-0531-9.Search in Google Scholar PubMed

12. Gupta, M, Mazumdar, UK, Sivakumar, T, Vamsi, ML, Karki, SS, Sambathkumar, R, et al.. Evaluation of anti-inflammatory activity of chloroform extract of Bryonia laciniosa in experimental animal models. Biol Pharm Bull 2003;26:1342–4. https://doi.org/10.1248/bpb.26.1342.Search in Google Scholar PubMed

13. Ahmed, KM, Rahman, SM, Alam, M, Mohammad, MM. Antinociceptive activity of Vitris trifolia. Fitoterapia 2007;62:276–7.Search in Google Scholar

14. Ansari, P, Debnath, M, Ahmad, MF, Azam, S, Akther, S, Mustakim, GM, et al.. Evaluation of antinociceptive activity of methanol extract from Cleome rutidosperma in mice. Chin Herb Med 2016;8:273–9. https://doi.org/10.1016/s1674-6384(16)60050-3.Search in Google Scholar

15. Ansari, P, Uddin, MJ, Akther, S, Azam, S, Mahmud, MK, Azad, SB, et al.. Investigation of antinociceptive activity of methanolic extract of Persicaria orientalis leaves in rodents. J Basic Clin Physiol Pharmacol 2017;28. https://doi.org/10.1515/jbcpp-2016-0018.Search in Google Scholar

16. Ogbole, OO, Adebayo, AG, Edith, OA. Ethnobotanical survey of plants used in treatment of inflammatory diseases in Ogun state of Nigeria. Eur J Sci Res 2010;43:183–91.Search in Google Scholar

17. Mohammadi-Motlagh, H, Ali, M, Kamran, M. Anticancer and anti-inflammatory activities of shallot (Allium ascalonicum) extract. Arch Med Sci 2011;7:38–44. https://doi.org/10.5114/aoms.2011.20602.Search in Google Scholar

18. Nasri, S, Mahdieh, A, Narges, K. Evaluation of analgesic and anti-inflammatory effects of fresh A. cepa juice in experimental animals. Asian J Pediatr Pract 2012;6:1679–84.10.5897/AJPP12.179Search in Google Scholar

19. Kalangutker, NXG, Joshi, PM. Pharmacological and behavioral comparative study of Allium cepa Linn. Bulb and coffee with Bhavana treatment in rats. JPIJ 2015;2:1–6.10.15406/ppij.2015.02.00024Search in Google Scholar

20. Wang, BS, Huang, GJ, Lu, YH, Chang, LW. Anti-inflammatory effects of an aqueous extract of Welsh A. cepa green leaves in mice. Food Chem 2013;138:751–6. https://doi.org/10.1016/j.foodchem.2012.11.106.Search in Google Scholar

21. Ranjan, S, Vikash, SJ, Nitish, S, Kamini, S, Varsha, P, Sanjay, G, et al.. Anti-inflammatory and analgesic potential of leaf extract of Allium stracheyi. JASR 2010;6:139–43.Search in Google Scholar

22. Hess, SM, Milonig, RC. Assay for anti-inflammatory drugs. In: Lepow, IH, Ward, PA, editors. Inflammation, mechanisms and control. New York: Academic Press; 1972:1–2 pp.Search in Google Scholar

23. Olajide, OA, Awe, SO, Makinde, JM. Studies on the anti-inflammatory, anti-pyretic and analgesic properties of Alstonia boonei stem bark. J Ethnopharmacol 2000;71:179–86. https://doi.org/10.1016/s0378-8741(99)00200-7.Search in Google Scholar

24. Olajide, OA, Makinde, JM, Okpako, DT, Awe, SO. Studies on the anti-inflammatory and related pharmacological properties of the aqueous extract of Bridelia ferruginea stem bark. J Ethnopharmacol 2000;71:153–60. https://doi.org/10.1016/s0378-8741(99)00201-9.Search in Google Scholar

25. Siegmund, E, Cadmus, R, Lu, G. A method for evaluating both non-narcotic and narcotic analgesics. Proc Soc Exp Biol Med 1957;95:729–31. https://doi.org/10.3181/00379727-95-23345.Search in Google Scholar PubMed

26. Carter, M, Shieh, JC. “Nociception”. Guide to Research Techniques in Neuroscience. Burlington, MA: Academic Press; 2010:51–2 pp. ISBN 978-0-12-374849-2.Search in Google Scholar

27. Duarte, DB, Vasko, MR, Fehrenbacher, JC. Models of inflammation: carrageenan air pouch. Curr Protoc Pharmacol 2012;5.6.1–5.6.8. https://doi.org/10.1002/0471141755.ph0506s56.Search in Google Scholar PubMed PubMed Central

28. Oyebanji, BO, Saba, AB, Oridupa, OA. Anti-inflammatory and analgesic effects of methanol extract of Stellaria media (L.) Vill leaf. Afr J Biomed Res 2012;15:181–6.Search in Google Scholar

29. Zeashana, H, Amresha, G, Raoa, CV, Singhb, S. Antinociceptive activity of Amaranthus spinosus in experimental animals. J Ethnopharmacol 2009;122:492–6. https://doi.org/10.1016/j.jep.2009.01.031.Search in Google Scholar PubMed

30. Garcia, MD, Fernández, MA, Alvarez, A, Saenz, MT. Antinociceptive and anti-inflammatory effect of the aqueous extract from leaves of Pimenta racemosa var. ozua (Mirtaceae). J Ethnopharmacol 2004;91:69–73. https://doi.org/10.1016/j.jep.2003.11.018.Search in Google Scholar PubMed

Received: 2020-03-24

Accepted: 2020-10-04

Published Online: 2021-02-08

You are currently not able to access this content.

Articles in the same Issue

https://doi.org/10.1515/jbcpp-2020-0080

Keywords for this article

analgesic; anti-inflammatory; mice; rats; red cultivar Allium cepa L