Quercetin modulates granulosa cell mRNA androgen receptor gene expression in dehydroepiandrosterone-induced polycystic ovary in Wistar rats via metabolic and hormonal pathways
-
Olugbemi T. Olaniyan
, Okoli Bamidele
Abstract
Background
It is estimated that about 5–10% of women suffer from polycystic ovarian syndrome (PCOS) which is a major cause of female reproductive dysfunction. This study examined the role of quercetin on dehydroepiandrosterone (DHEA)-induced PCO in Wistar rats.
Methods
Twenty-eight pre-pubertal female Wistar rats that are 21 days old weighing 16–21 g were sorted into four groups (n = 7). Group I served as control and was given distilled water only, Group II were injected with 6 mg/100 g BW of DHEA in 0.2 mL of corn oil subcutaneously, Group III received 100 mg/kg BW of quercetin orally and Group IV received 6 mg/100 g BW of DHEA in 0.2 mL of corn oil subcutaneously and 100 mg/kg BW of quercetin orally. Rats were sacrificed after 15 days by cervical dislocation method. Blood samples and ovaries were collected for hormonal, biochemical, and histopathological analysis and expressions of mRNA androgen receptor gene were determined using RT–qPCR. All data were analysed using one-way ANOVA.
Results
A significant decrease (p < 0.05) in the antioxidant and metabolic enzyme activity in the DHEA treated group was observed when compared with control. DHEA co-administration with quercetin showed a significant decrease in malondialdehyde and cytokines when compared with DHEA treated group. Also a significant increase in progesterone, metabolic and antioxidant enzyme activity was observed. The histopathology demonstrates a reduction in cystic and atretic cells, improved expression of BCl2, E-Cadherin and a decrease in Bax.
Conclusions
Quercetin alleviated DHEA-induced PCO. These effects could be attributed to its antioxidant property.
Acknowledgments
We thank Mr. Olumide Afolabi and African Biosciences Ltd for providing laboratory facilities and technical support for the execution of the gene expression study of this research. Also, we appreciate Solis Biodyne Ltd Estonia for supporting us with the extraction Kitz.
Research funding: The research did not receive any external funding (None declared).
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Ethical approval: The ethical approval on the animal act right was obtained from the Institutional Animal Care Committee of the same Institution. The experimental procedures were carried out in accordance with Institutional Animal Ethics Committee (IAEC) guidelines.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Editorial
- Anti-microbial stewardship: is it old wine in a new bottle?
- Reviews
- Tangeretin: a mechanistic review of its pharmacological and therapeutic effects
- Silymarin: not just another antioxidant
- Original Articles
- Melatonin ameliorates some biochemical alterations following ketoconazole administration in rats
- Quercetin modulates granulosa cell mRNA androgen receptor gene expression in dehydroepiandrosterone-induced polycystic ovary in Wistar rats via metabolic and hormonal pathways
- Comparative expression analysis of phospholipid binding protein annexina1 in nephrogenesis and kidney cancer
- Anthonotha macrophylla P. Beauv (Caesalpiniaceae) aqueous extract exhibits antiestrogenic effects in vitro and in vivo
- Urate crystals trigger B-cell receptor signal transduction and induce B-cell proliferation
- Effects of gabapentinoids on responses of primary cultures from rat dorsal root ganglia to inflammatory or somatosensory stimuli
- Anti-inflammatory potential of chia seeds oil and mucilage against adjuvant-induced arthritis in obese and non-obese rats
- Antiproliferative effects of combinational therapy of Lycopodium clavatum and quercetin in colon cancer cells
- Attenuation of oxidative stress and neurotoxicity involved in the antidepressant-like effect of the MK-801(dizocilpine) in Bacillus Calmette-Guerin-induced depression in mice
- 3-(Para-fluorobenzoyl)-propionic acid; a metabolite of haloperidol, reversed oestradiol valerate-induced uterine hyperplasia via modulation of oestrogen receptor signalling pathways in female Wistar rats
- Pharmacological evidence of Vitex thyrsiflora, Entandrophragma cylindricum, and Anonidium mannii used for the management of inflammation in Cameroon
- Mitigation of aflatoxin B1- and sodium arsenite-induced cytotoxicities in HUC-PC urinary bladder cells by curcumin and Khaya senegalensis
- Saccharum officinarum juice alters reproductive functions in male Wistar rats
- The effects of Pb on sperm parameters and sperm DNA fragmentation of men investigated for infertility
Artikel in diesem Heft
- Editorial
- Anti-microbial stewardship: is it old wine in a new bottle?
- Reviews
- Tangeretin: a mechanistic review of its pharmacological and therapeutic effects
- Silymarin: not just another antioxidant
- Original Articles
- Melatonin ameliorates some biochemical alterations following ketoconazole administration in rats
- Quercetin modulates granulosa cell mRNA androgen receptor gene expression in dehydroepiandrosterone-induced polycystic ovary in Wistar rats via metabolic and hormonal pathways
- Comparative expression analysis of phospholipid binding protein annexina1 in nephrogenesis and kidney cancer
- Anthonotha macrophylla P. Beauv (Caesalpiniaceae) aqueous extract exhibits antiestrogenic effects in vitro and in vivo
- Urate crystals trigger B-cell receptor signal transduction and induce B-cell proliferation
- Effects of gabapentinoids on responses of primary cultures from rat dorsal root ganglia to inflammatory or somatosensory stimuli
- Anti-inflammatory potential of chia seeds oil and mucilage against adjuvant-induced arthritis in obese and non-obese rats
- Antiproliferative effects of combinational therapy of Lycopodium clavatum and quercetin in colon cancer cells
- Attenuation of oxidative stress and neurotoxicity involved in the antidepressant-like effect of the MK-801(dizocilpine) in Bacillus Calmette-Guerin-induced depression in mice
- 3-(Para-fluorobenzoyl)-propionic acid; a metabolite of haloperidol, reversed oestradiol valerate-induced uterine hyperplasia via modulation of oestrogen receptor signalling pathways in female Wistar rats
- Pharmacological evidence of Vitex thyrsiflora, Entandrophragma cylindricum, and Anonidium mannii used for the management of inflammation in Cameroon
- Mitigation of aflatoxin B1- and sodium arsenite-induced cytotoxicities in HUC-PC urinary bladder cells by curcumin and Khaya senegalensis
- Saccharum officinarum juice alters reproductive functions in male Wistar rats
- The effects of Pb on sperm parameters and sperm DNA fragmentation of men investigated for infertility