The standardized extract of Nigella sativa and its major ingredient, thymoquinone, ameliorates angiotensin II-induced hypertension in rats
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Lili Enayatfard
Abstract
Background
This study investigated the effect of hydroalcoholic extract of Nigella sativa (N. sativa) and its active component, thymoquinone (TQ) on hypertension induced by angiotensin II (AngII), the main product of renin–angiotensin system (RAS).
Methods
Seven animal groups (n=7 for each group) were used as follows: (1) control, (2) AngII (300 ng/kg), (3) AngII+losartan (Los; 10 mg/kg), (4) TQ (40 mg/kg)+AngII, and (5–7) three doses of N. sativa (200, 400, and 600 mg/kg)+AngII. Los and AngII were injected intravenously; TQ and extracts were injected intraperitoneally. In TQ and N. sativa-treated groups, 30 min after injection of the extract and TQ, AngII was injected. Cardiovascular parameters were recorded by power lab system after cannulation of femoral artery. The maximum changes (∆) of systolic blood pressure (SBP), mean arterial pressure (MAP), and heart rate (HR) were calculated and used for statistical analysis.
Results
AngII significantly increased maximal ∆SBP, ∆MAP, and ∆HR compared with the control (p<0.001), and these effects significantly were blunted by Los. TQ and two higher doses (400 and 600 mg/kg) of N. sativa significantly could antagonize effect of AngII on ∆SBP, ∆MAP (p<0.05 to p<0.001). AngII-induced changes of HR are also significantly decreased by TQ and dose 600 mg/kg of extract (p<0.01 and p<0.05, respectively).
Conclusions
The N. sativa and its component TQ have the beneficial effect on hypertension probably due to attenuation cardiovascular effects of AngII.
Acknowledgments
The authors would like to thank the Vice Presidency of Research Center of Mashhad University of Medical Sciences for their financial support.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
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- Behavior and Neuroprotection
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- Pubertal anabolic androgenic steroid exposure in male rats affects levels of gonadal steroids, mating frequency, and pregnancy outcome
- Dynamics of inflammatory reaction and oxidative stress across maternal serum, placenta and amniotic fluid in laboratory rats and the role played by genistein aglycone
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- Local inhibition of carbonic anhydrase does not decrease sweat rate
- The standardized extract of Nigella sativa and its major ingredient, thymoquinone, ameliorates angiotensin II-induced hypertension in rats
- Correlation between the blood level of irisin and the severity of acute myocardial infarction in exercise-trained rats
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- Pravastatin attenuates testicular damage induced by doxorubicin – a stereological and histopatological study
- Metabolism
- Re-establishing normal diet following high fat-diet-induced obesity reverses the altered salivary composition in Wistar rats
- Phytotherapy
- In vivo analgesic effect of different extracts of Hopea odorata leaves in mice and in silico molecular docking and ADME/T property analysis of some isolated compounds from this plant
- Hepatoprotective activity of Tamarindus indica Linn stem bark ethanolic extract against hepatic damage induced by co-administration of antitubercular drugs isoniazid and rifampicin in Sprague Dawley rats
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Articles in the same Issue
- Frontmatter
- Reviews
- Immune response and inflammatory pathway of ulcerative colitis
- Current insights and future perspectives of hypoxia-inducible factor-targeted therapy in cancer
- Behavior and Neuroprotection
- Effect of caffeine on alcohol consumption and alcohol-induced conditioned place preference in rodents
- Reproduction
- Pubertal anabolic androgenic steroid exposure in male rats affects levels of gonadal steroids, mating frequency, and pregnancy outcome
- Dynamics of inflammatory reaction and oxidative stress across maternal serum, placenta and amniotic fluid in laboratory rats and the role played by genistein aglycone
- Cardiovascular-Pulmonary Interactions
- Local inhibition of carbonic anhydrase does not decrease sweat rate
- The standardized extract of Nigella sativa and its major ingredient, thymoquinone, ameliorates angiotensin II-induced hypertension in rats
- Correlation between the blood level of irisin and the severity of acute myocardial infarction in exercise-trained rats
- Oxidative Stress
- Zataria multiflora extract and carvacrol affect cardiotoxicity induced by Adriamycin in rat
- Attenuation of oxidative stress and hepatic damage by white butterfly (Clerodendrum volubile) leaves in streptozotocin-induced diabetes in rats
- Proline supplementation mitigates the early stage of liver injury in bile duct ligated rats
- Pravastatin attenuates testicular damage induced by doxorubicin – a stereological and histopatological study
- Metabolism
- Re-establishing normal diet following high fat-diet-induced obesity reverses the altered salivary composition in Wistar rats
- Phytotherapy
- In vivo analgesic effect of different extracts of Hopea odorata leaves in mice and in silico molecular docking and ADME/T property analysis of some isolated compounds from this plant
- Hepatoprotective activity of Tamarindus indica Linn stem bark ethanolic extract against hepatic damage induced by co-administration of antitubercular drugs isoniazid and rifampicin in Sprague Dawley rats
- Case Report
- Drug-induced optic neuropathy in a case of extensively drug-resistant pulmonary tuberculosis
