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Proline supplementation mitigates the early stage of liver injury in bile duct ligated rats

  • Reza Heidari EMAIL logo , Hamidreza Mohammadi , Vahid Ghanbarinejad , Asrin Ahmadi , Mohammad Mehdi Ommati , Hossein Niknahad , Akram Jamshidzadeh , Negar Azarpira and Narges Abdoli EMAIL logo
Published/Copyright: September 6, 2018
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 30 Issue 1

Abstract

Background

Proline is a proteinogenic amino acid with multiple biological functions. Several investigations have been supposed that cellular proline accumulation is a stress response mechanism. This amino acid acts as an osmoregulator, scavenges free radical species, boosts cellular antioxidant defense mechanisms, protects mitochondria, and promotes energy production. The current study was designed to investigate the effect of proline treatment on the liver in bile duct ligated (BDL) rats as an animal model of cholestasis/cirrhosis.

Methods

BDL rats were supplemented with proline-containing drinking water (0.25% and 0.5% w:v), and samples were collected at scheduled time intervals (3, 7, 14, 28, and 42 days after BDL surgery).

Results

Drastic elevation in the serum level of liver injury biomarkers and significant tissue histopathological changes were evident in BDL rats. Markers of oxidative stress were also higher in the liver of BDL animals. It was found that proline supplementation attenuated BDL-induced alteration in serum biomarkers of liver injury, mitigated liver histopathological changes, and alleviated markers of oxidative stress at the early stage of BDL operation (3, 7, and 14 days after BDL surgery).

Conclusions

The hepatoprotection provided by proline in BDL animals might be associated with its ability to attenuate oxidative stress and its consequences.

Keywords: amino acid; cirrhosis; hepatoprotective; liver fibrosis; oxidative stress

Acknowledgments

The instrumental facility provided from Pharmaceutical Sciences Research Center of Shiraz University of Medical Sciences is gratefully acknowledged. The current investigation was financially supported by the Vice-Chancellor for Research Affairs of Shiraz University of Medical Sciences (Funder Id: 10.13039/501100004320, Grant number: 95-01-36-13555).

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: Shiraz University of Medical Sciences (95-01-36-13555).

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2018-01-05
Accepted: 2018-07-11
Published Online: 2018-09-06
Published in Print: 2018-12-19

©2019 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2017-0221
Keywords for this article
amino acid; cirrhosis; hepatoprotective; liver fibrosis; oxidative stress

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Immune response and inflammatory pathway of ulcerative colitis
  4. Current insights and future perspectives of hypoxia-inducible factor-targeted therapy in cancer
  5. Behavior and Neuroprotection
  6. Effect of caffeine on alcohol consumption and alcohol-induced conditioned place preference in rodents
  7. Reproduction
  8. Pubertal anabolic androgenic steroid exposure in male rats affects levels of gonadal steroids, mating frequency, and pregnancy outcome
  9. Dynamics of inflammatory reaction and oxidative stress across maternal serum, placenta and amniotic fluid in laboratory rats and the role played by genistein aglycone
  10. Cardiovascular-Pulmonary Interactions
  11. Local inhibition of carbonic anhydrase does not decrease sweat rate
  12. The standardized extract of Nigella sativa and its major ingredient, thymoquinone, ameliorates angiotensin II-induced hypertension in rats
  13. Correlation between the blood level of irisin and the severity of acute myocardial infarction in exercise-trained rats
  14. Oxidative Stress
  15. Zataria multiflora extract and carvacrol affect cardiotoxicity induced by Adriamycin in rat
  16. Attenuation of oxidative stress and hepatic damage by white butterfly (Clerodendrum volubile) leaves in streptozotocin-induced diabetes in rats
  17. Proline supplementation mitigates the early stage of liver injury in bile duct ligated rats
  18. Pravastatin attenuates testicular damage induced by doxorubicin – a stereological and histopatological study
  19. Metabolism
  20. Re-establishing normal diet following high fat-diet-induced obesity reverses the altered salivary composition in Wistar rats
  21. Phytotherapy
  22. In vivo analgesic effect of different extracts of Hopea odorata leaves in mice and in silico molecular docking and ADME/T property analysis of some isolated compounds from this plant
  23. Hepatoprotective activity of Tamarindus indica Linn stem bark ethanolic extract against hepatic damage induced by co-administration of antitubercular drugs isoniazid and rifampicin in Sprague Dawley rats
  24. Case Report
  25. Drug-induced optic neuropathy in a case of extensively drug-resistant pulmonary tuberculosis
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