Attenuation of oxidative stress and hepatic damage by white butterfly (Clerodendrum volubile) leaves in streptozotocin-induced diabetes in rats

Olorunfemi R. Molehin; Omotade I. Oloyede

doi:10.1515/jbcpp-2018-0083

Article

Attenuation of oxidative stress and hepatic damage by white butterfly (Clerodendrum volubile) leaves in streptozotocin-induced diabetes in rats

Olorunfemi R. Molehin and Omotade I. Oloyede

Published/Copyright: August 22, 2018

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 30 Issue 1

Abstract

Background

The negative impact of diabetes on the liver is well documented. The white butterfly (Clerodendrum volubile) leaf has been used in traditional practices for the treatment of various diseases, such as hypertension, diabetes, and rheumatism, but without scientific validation. This work was designed to evaluate the hepatoprotective properties of Clerodendrum volubile leaves on oxidative stress in streptozotocin (STZ)-induced diabetes in rats.

Methods

The rats were divided into ten groups of five rats each. Diabetes was induced by a single injection of STZ (65 mg/kg body weight; i.p), while the C. volubile extract (at the respective doses of 50, 100 and 200 mg/kg body weight) was given to diabetic and non-diabetic rats orally for 14 days. Metformin (100 mg/kg body weight) served as the positive control. Biochemical assays were conducted on the plasma for hematological parameters, along with hepatic marker damages and antioxidant enzyme determination in vivo to assess hepatic injury.

Results

The diabetic control rats showed significant increase (p<0.05) in marker enzymes: aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and malondiadehyde with reduction in reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase activities and plasma total protein content. Likewise, there were alterations in hematological indices in the diabetic control rats when compared with the normal control. However, treatment with C. volubile caused a reversal of the above parameters towards normal levels, thereby suggesting the modulating effect of the extract on oxidative stress, which may be a result of the high polyphenolic content and antioxidant capacity.

Conclusions

The protection of the liver tissues and the modulation of oxidative stress in STZ diabetic rats compare favorably to metformin, a standard antidiabetic drug.

Keywords: antioxidan; Clerodendrum volubile; diabetes mellitus; hepatotoxicity; histopathology; oxidative stress

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by the Ekiti State University, Ado-Ekiti Needs Assessment Intervention Training Scholarship under the Implementation Monitoring Committee of the Federal Ministry of Education, Nigeria awarded to Mr. O.R. Molehin, Grant Number: VC/NADO/VOLII/04.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2018-02-08

Accepted: 2018-07-06

Published Online: 2018-08-22

Published in Print: 2018-12-19

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2018-0083

Keywords for this article

antioxidan; Clerodendrum volubile; diabetes mellitus; hepatotoxicity; histopathology; oxidative stress