Antidiabetic and renoprotective effect of Anogeissus acuminata leaf extract on experimentally induced diabetic nephropathy
-
Archana M. Navale
and
Archana Paranjape
Abstract
Background
Diabetic nephropathy is the leading cause of end-stage renal disease. Hyperglycemia, oxidative stress, and inflammation are some of the mechanisms involved in renal damage. Anogeissus acuminata (AA) is used in India as an antidiabetic agent and has potent antioxidant activity. However, it has never been evaluated for its effect on diabetic nephropathy. Hence, in the present study we aimed to evaluate its effect on streptozotocin-induced diabetes mellitus and its renal complications.
Methods
Diabetes mellitus was induced by injecting streptozotocin, 50 mg/kg, i.p. in rats fasted for 6 h. Rats with hyperglycemia were treated with extracts of AA for 8 weeks at doses of 100 and 300 mg/kg, orally. Human NPH insulin (4 IU/kg, s.c.) was used as standard treatment. Plasma glucose levels (at weeks 1, 2, 4, and 8) and oxidative stress parameters (at weeks 2 and 4) were assessed. Effect on diabetic nephropathy was evaluated by recording the urinary volume, urinary protein excretion, kidney weights, serum creatinine, and blood urea nitrogen levels at week 8.
Results
Methanolic extract of AA leaves produced statistically significant (p<0.05) hypoglycemic and antioxidant effect. It also resulted in improved urinary function, reflected by better urinary volume and reduced protein excretion in urine. AA treatment could prevent the elevation of serum creatinine and blood urea nitrogen level in a dose-dependent manner. Kidney hypertrophy could be attenuated remarkably, as reflected by the significantly lower kidney weight (KW) per 100 g body weight (p<0.05).
Conclusions
AA leaf extract attenuated the development of diabetic nephropathy and also demonstrated antidiabetic and antioxidant action.
Author contributions: All authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. DeFronzo RA. Diabetic nephropathy: etiologic and therapeutic considerations. Diabetes Rev 1995;3:510–64.Search in Google Scholar
2. Pullaiah K, Naidu C. Antidiabetic plants in India and herbal based antidiabetic research, 2nd ed. New Delhi: Regency Publications, 2003:86–7.Search in Google Scholar
3. Manosroi J, Moses ZZ, Manosroi W, Manosroi A. Hypoglycemic activity of Thai medicinal plants selected from the Thai/Lanna Medicinal Recipe Database MANOSROI II. J Ethnopharmacol 2011;138:92–8.10.1016/j.jep.2011.08.049Search in Google Scholar
4. Navale AM, Paranjape AN. Phytochemical screening and HPTLC fingerprinting of Anogeissus acuminata extracts. Int J Res Ayurveda Pharm 2016;7:207–9.10.7897/2277-4343.07288Search in Google Scholar
5. Hemamalini K, Vijusha M. Antidiabetic activity of methanolic extracts of leaves of Anogeissus acuminata Roxburgh ex candolle and Solanum pubescens Willd by alloxan induced model in rats. Pharm Lett 2012;4:1445–60.Search in Google Scholar
6. Zaruwa MZ, Manosroi J, Akihisa T, Manosroi A. Castalagin from Anogeissus acuminata (Roxb. ex. DC) Guill. Ex. Perr, a potent hypoglycaemic agent. Int J Curr Res Acad Rev 2015;3:147–52.Search in Google Scholar
7. Navale AM, Paranjape AN. In vitro antioxidant and PTP inhibitory activity of methanolic extract of Anogeissus acuminata leaf and bark. J Pharm Res 2016;10:65–8.Search in Google Scholar
8. Aebi H. Catalase. In: Bergmeyer HU, editor. Methods of enzymatic analysis. New York: Academic Press Inc, 1974:673–84.10.1016/B978-0-12-091302-2.50032-3Search in Google Scholar
9. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882–8.Search in Google Scholar
10. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.10.1016/0003-2697(79)90738-3Search in Google Scholar
11. Kaminskas A, Mazeikiene A. Biochemistry laboratory manual, 1st ed. [ebook]. Vilnius: Vilnius University, Faculty of Medicine, 2012: 9. Available at: http://www.fblm.mf.vu.lt/wpcontent/uploads/2014/02/Lab-darbai-Angliskai.pdf. Accessed 09 Jan 2014.Search in Google Scholar
12. Matsumoto K, Hatano M. Production of interleukin 1 in glomerular cell cultures from rats with nephrotoxic serum nephritis. Clin Exp Immunol 1989;75:123.Search in Google Scholar
13. Shen SC, Chang WC. Hypotriglyceridemic and hypoglycemic effects of vescalagin from Pink wax apple [Syzygium samarangense (Blume) Merrill and Perry cv. Pink] in high-fructose diet-induced diabetic rats. Food Chem 2013;136:858–63.10.1016/j.foodchem.2012.08.037Search in Google Scholar
14. Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozocin-induced diabetic rats. Comp Biochem Physiol C Toxicol Pharmacol 2003;135:357–64.10.1016/S1532-0456(03)00140-6Search in Google Scholar
15. Coskun O, Kanter M, Korkmaz A, Oter S. Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacol Res 2005;51:117–23.10.1016/j.phrs.2004.06.002Search in Google Scholar PubMed
16. Abdelmoaty MA, Ibrahim MA, Ahmed NS, Abdelaziz MA. Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats. Indian J Clin Biochem 2010;25:188–92.10.1007/s12291-010-0034-xSearch in Google Scholar PubMed PubMed Central
17. Arora MK, Singh UK. Molecular mechanisms in the pathogenesis of diabetic nephropathy: An update. vascul Pharmacol 2013;58:259–71.10.1016/j.vph.2013.01.001Search in Google Scholar PubMed
18. Cao Z, Cooper ME. Pathogenesis of diabetic nephropathy. J Diabetes Investig 2011;2:243–7.10.1111/j.2040-1124.2011.00131.xSearch in Google Scholar PubMed PubMed Central
19. De Zeeuw D, Ramjit D, Zhang Z, Ribeiro AB, Kurokawa K, Lash JP, et al. Renal risk and renoprotection among ethnic groups with type 2 diabetic nephropathy: a post hoc analysis of RENAAL. Kidney Int 2006;69:1675–82.10.1038/sj.ki.5000326Search in Google Scholar PubMed
20. Nakamura Y, Myers BD. Charge selectivity of proteinuria in diabetic glomerulopathy. Diabetes 1988;37:1202–11.10.2337/diab.37.9.1202Search in Google Scholar PubMed
21. Pietravalle P, Morano S, Cristina G, De Rossi MG, Mariani G, Cotroneo P, et al. Charge selectivity of proteinuria in type I diabetes explored by Ig subclass clearance. Diabetes 1991;40:1685–90.10.2337/diab.40.12.1685Search in Google Scholar PubMed
22. Morano S, Pietravalle P, De Rossi MG, Mariani G, Cristina G, Medici F, et al. A charge selectivity impairment in protein permselectivity is present in type 2 diabetes. Acta Diabetol 1993;30:138–42.10.1007/BF00572857Search in Google Scholar PubMed
23. Ruggenenti P, Remuzzi G. Time to abandon microalbuminuria? Kidney Int 2006;70:1214–22.10.1038/sj.ki.5001729Search in Google Scholar PubMed
24. Dinneen SF, Gerstein HC. The association of microalbuminuria and mortality in non-insulin-dependent diabetes mellitus: a systematic overview of the literature. Arch Intern Med 1997;157:1413–8.10.1001/archinte.1997.00440340025002Search in Google Scholar
25. Gomes IB, Porto ML, Santos MC, Campagnaro BP, Pereira TM, Meyrelles SS, et al. Renoprotective, anti-oxidative and anti-apoptotic effects of oral low-dose quercetin in the C57BL/6J model of diabetic nephropathy. Lipids Health Dis 2014;13:184.10.1186/1476-511X-13-184Search in Google Scholar PubMed PubMed Central
26. ArunaDevi R, Lata S, Bhadoria BK, Ramteke VD, Kumar S, Sankar P, et al. Neuroprotective effect of 5, 7, 3′, 4′, 5′-pentahydroxy dihdroflavanol-3-O-(2″-O-galloyl)-β-d-glucopyranoside, a polyphenolic compound in focal cerebral ischemia in rat. Eur J Pharmacol 2010;626:205–12.10.1016/j.ejphar.2009.09.038Search in Google Scholar PubMed
©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Letter to the Editor
- Blister-packed levothyroxine sodium or bottle-packed levothyroxine sodium
- Metabolism
- Comparative hypoglycemic potentials and phytochemical profiles of 12 common leafy culinary vegetables consumed in Nsukka, Southeastern Nigeria
- Infection
- The effects of oral pregabalin on post-Lichtenstein herniorrhaphic pain: a randomized clinical trial
- Cardiovascular Function
- Influence of age and gender on blood pressure variability and baroreflex sensitivity in a healthy population in the Indian sub-continent
- Autonomic responses during acute myocardial infarction in the rat model: implications for arrhythmogenesis
- Phytotherapy
- Role of reactive oxygen species–total antioxidant capacity status in Telfairia occidentalis leaves–associated spermatoprotective effect: a pointer to fatty acids benefit
- Antidiabetic and renoprotective effect of Anogeissus acuminata leaf extract on experimentally induced diabetic nephropathy
- Oxidative Stress
- Aged coconut oil with a high peroxide value induces oxidative stress and tissue damage in mercury-treated rats
- Anti-ulcerogenic effect of the methanol extract of Chasmanthera dependens (Hochst) stem on male Wistar rats
- Hematological Profile
- Evaluation of hematological alterations after therapeutic use of dipyrone in healthy adults: a prospective study
- Hematology and erythrocyte osmotic fragility of the Franquet’s fruit bat (Epomops franqueti)
- Behavior and Neuroprotection
- Haloperidol-induced parkinsonism is attenuated by varenicline in mice
- Antioxidant-mediated neuroprotection by Allium schoenoprasum L. leaf extract against ischemia reperfusion-induced cerebral injury in mice
- Effects of acetone extract of Cola nitida on brain sodium-potassium adenosine triphosphatase activity and spatial memory in healthy and streptozotocin-induced diabetic female Wistar rats
- Case Report
- Conservative treatment of postoperative chylothorax: a case report
- Short Communication
- Improved serotonergic neurotransmission by genistein pretreatment regulates symptoms of obsessive-compulsive disorder in streptozotocin-induced diabetic mice
Articles in the same Issue
- Frontmatter
- Letter to the Editor
- Blister-packed levothyroxine sodium or bottle-packed levothyroxine sodium
- Metabolism
- Comparative hypoglycemic potentials and phytochemical profiles of 12 common leafy culinary vegetables consumed in Nsukka, Southeastern Nigeria
- Infection
- The effects of oral pregabalin on post-Lichtenstein herniorrhaphic pain: a randomized clinical trial
- Cardiovascular Function
- Influence of age and gender on blood pressure variability and baroreflex sensitivity in a healthy population in the Indian sub-continent
- Autonomic responses during acute myocardial infarction in the rat model: implications for arrhythmogenesis
- Phytotherapy
- Role of reactive oxygen species–total antioxidant capacity status in Telfairia occidentalis leaves–associated spermatoprotective effect: a pointer to fatty acids benefit
- Antidiabetic and renoprotective effect of Anogeissus acuminata leaf extract on experimentally induced diabetic nephropathy
- Oxidative Stress
- Aged coconut oil with a high peroxide value induces oxidative stress and tissue damage in mercury-treated rats
- Anti-ulcerogenic effect of the methanol extract of Chasmanthera dependens (Hochst) stem on male Wistar rats
- Hematological Profile
- Evaluation of hematological alterations after therapeutic use of dipyrone in healthy adults: a prospective study
- Hematology and erythrocyte osmotic fragility of the Franquet’s fruit bat (Epomops franqueti)
- Behavior and Neuroprotection
- Haloperidol-induced parkinsonism is attenuated by varenicline in mice
- Antioxidant-mediated neuroprotection by Allium schoenoprasum L. leaf extract against ischemia reperfusion-induced cerebral injury in mice
- Effects of acetone extract of Cola nitida on brain sodium-potassium adenosine triphosphatase activity and spatial memory in healthy and streptozotocin-induced diabetic female Wistar rats
- Case Report
- Conservative treatment of postoperative chylothorax: a case report
- Short Communication
- Improved serotonergic neurotransmission by genistein pretreatment regulates symptoms of obsessive-compulsive disorder in streptozotocin-induced diabetic mice
