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Antioxidant-mediated neuroprotection by Allium schoenoprasum L. leaf extract against ischemia reperfusion-induced cerebral injury in mice

  • Varinder Singh , Pawan Krishan and Richa Shri EMAIL logo
Published/Copyright: June 22, 2018
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 29 Issue 4

Abstract

Background

Oxidative stress is strongly implicated in ischemia reperfusion (IR)-mediated functional and neuronal impairment. Therefore, strategies employing antioxidants to reverse the damage due to IR are being investigated. Allium schoenoprasum L. is a culinary medicine whose antioxidant properties are well documented but whose neuroprotective potential has not been examined. Hence, the present study was designed to evaluate the effect of A. schoenoprasum leaf extract (ASLE) on functional deficit against IR-induced cerebral injury in mice.

Methods

Acute toxicity studies of ASLE were performed following the Organisation for Economic Co-operation and Development Guideline 423. IR injury was induced by bilateral common carotid artery occlusion (BCCAO) for 15 min followed by 24-h reperfusion. Animals were treated for 7 days with ASLE (200 and 400 mg/kg, p.o. once daily) after IR injury. Functional outcomes (memory and sensorimotor functions) were measured using Morris water maze and neurological severity score, respectively. Cerebral infarct size and oxidative stress (thiobarbituric acid reactive species (TBARS), reduced glutathione (GSH), and superoxide dismutase (SOD) activity) were measured in order to elucidate the neuroprotective mechanism of ASLE.

Results

No toxic effects of ASLE were observed in mice. Oral treatment with ASLE for 7 days significantly attenuated IR-mediated memory and sensorimotor function deficit in the animals. The extract also reduced the cerebral infarct size and rise in brain TBARS levels, and restored the GSH levels and SOD activity.

Conclusions

The results of the present study suggest that ASLE is safe and effective in improving functional outcomes. It demonstrates neuroprotective effect by enhancing the antioxidant defence against IR injury.

Keywords: Allium schoenoprasum L.; antioxidant; ischemia reperfusion; memory; neuroprotection; sensorimotor function

Funding source: University Grants Commission

Award Identifier / Grant number: No.F.7-265/2009(BSR)

Funding statement: The authors wish to thank Prof. Nirmal Singh, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, India, for providing lab facilities and guidance in the pharmacological studies. VR thanks the University Grants Commission, New Delhi, India, for awarding a UGC-BSR research fellowship [No.F.7-265/2009(BSR)] for carrying out this research work, funder Id: 10.13039/501100001501.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organisation(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2017-05-08
Accepted: 2017-12-02
Published Online: 2018-06-22
Published in Print: 2018-07-26

©2018 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2017-0070
Keywords for this article
Allium schoenoprasum L.; antioxidant; ischemia reperfusion; memory; neuroprotection; sensorimotor function

Articles in the same Issue

  1. Frontmatter
  2. Letter to the Editor
  3. Blister-packed levothyroxine sodium or bottle-packed levothyroxine sodium
  4. Metabolism
  5. Comparative hypoglycemic potentials and phytochemical profiles of 12 common leafy culinary vegetables consumed in Nsukka, Southeastern Nigeria
  6. Infection
  7. The effects of oral pregabalin on post-Lichtenstein herniorrhaphic pain: a randomized clinical trial
  8. Cardiovascular Function
  9. Influence of age and gender on blood pressure variability and baroreflex sensitivity in a healthy population in the Indian sub-continent
  10. Autonomic responses during acute myocardial infarction in the rat model: implications for arrhythmogenesis
  11. Phytotherapy
  12. Role of reactive oxygen species–total antioxidant capacity status in Telfairia occidentalis leaves–associated spermatoprotective effect: a pointer to fatty acids benefit
  13. Antidiabetic and renoprotective effect of Anogeissus acuminata leaf extract on experimentally induced diabetic nephropathy
  14. Oxidative Stress
  15. Aged coconut oil with a high peroxide value induces oxidative stress and tissue damage in mercury-treated rats
  16. Anti-ulcerogenic effect of the methanol extract of Chasmanthera dependens (Hochst) stem on male Wistar rats
  17. Hematological Profile
  18. Evaluation of hematological alterations after therapeutic use of dipyrone in healthy adults: a prospective study
  19. Hematology and erythrocyte osmotic fragility of the Franquet’s fruit bat (Epomops franqueti)
  20. Behavior and Neuroprotection
  21. Haloperidol-induced parkinsonism is attenuated by varenicline in mice
  22. Antioxidant-mediated neuroprotection by Allium schoenoprasum L. leaf extract against ischemia reperfusion-induced cerebral injury in mice
  23. Effects of acetone extract of Cola nitida on brain sodium-potassium adenosine triphosphatase activity and spatial memory in healthy and streptozotocin-induced diabetic female Wistar rats
  24. Case Report
  25. Conservative treatment of postoperative chylothorax: a case report
  26. Short Communication
  27. Improved serotonergic neurotransmission by genistein pretreatment regulates symptoms of obsessive-compulsive disorder in streptozotocin-induced diabetic mice
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