In vitro ovicidal activity of poly lactic acid curcumin-nisin co-entrapped nanoparticle against Fasciola spp. eggs and its reproductive toxicity

Oyetunde Oyeyemi; Odunayo Adegbeyeni; Ifeoluwa Oyeyemi; Jairam Meena; Amulya Panda

doi:10.1515/jbcpp-2017-0045

Article

In vitro ovicidal activity of poly lactic acid curcumin-nisin co-entrapped nanoparticle against Fasciola spp. eggs and its reproductive toxicity

Oyetunde Oyeyemi , Odunayo Adegbeyeni , Ifeoluwa Oyeyemi , Jairam Meena and Amulya Panda

Published/Copyright: October 10, 2017

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 29 Issue 1

Abstract

Background:

Curcumin and nisin have been widely reported for their antibacterial and anticancer potency. However, their therapeutic applications are hampered by several factors, which necessitate their development into nanosize ranges for improved delivery and activities. Their incorporation into a single nanosynthesized form may suggest desirable efficacy on parasites. The aim of the study was to assess the ovicidal activity of the curcumin-nisin polylactic acid (PLA) entrapped nanoparticle on the Fasciola eggs and its reproductive toxicity.

Methods:

The nanoparticle was formulated by double emulsion method. The eggs of the adult Fasciola spp. were exposed to different concentrations (0.3125–5 mg/mL) of the nanoparticle to monitor hatchability. Mice were exposed to 0.5 mL of the formulated drug at varying concentrations (10–20 mg/kg) and then sacrificed for sperm morphology assay.

Results:

The mean particle size, polydispersity index, and drug entrapment efficiency of the formulated drug were 288.4±24.3 nm, 0.232, and 51.7%, respectively. The highest nanoparticulate concentration (5 mg/mL) showed the least percentage egg hatching (41.7%) compared with the other treatment groups and positive control (albendazole) (45.1%). The aberrations observed in sperm cells were not concentration-dependent and no significant differences were observed in the mean aberrations between the nanoparticulate drug-exposed groups and the negative control (p>0.05).

Conclusions:

The results confirmed the ovicidal activity of the curcumin-nisin nanoparticulate drug against the Fasciola species. The formulation also showed no toxicity to sperm cells. More robust studies on anti-fascioliasis activity of the drug on adult Fasciola spp. and in vivo and in vitro toxicity studies are recommended.

Keywords: curcumin; Fasciola species; nanomedicine; nisin; properties; toxicity

Acknowledgments

OO acknowledges the Centre for Science and Technology of the Non-Aligned and Other Developing Countries (NAM S&T Centre) in collaboration with the Department of Science and Technology (DST), Government of India for ‘Research Training Fellowship for Developing Country Scientists (RTF-DCS)’ award given at the the National Institute of Immunology, New Delhi, India.

Author’s contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2017-4-7

Accepted: 2017-8-23

Published Online: 2017-10-10

Published in Print: 2018-1-26

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2017-0045

Keywords for this article

curcumin; Fasciola species; nanomedicine; nisin; properties; toxicity