Anti-invasive and antiproliferative effects of Pleurotus ostreatus extract on acute leukemia cell lines
-
Alireza Ebrahimi
,
Masoud Soleimani
Abstract
Background:
Currently, mushrooms have been used in traditional and folk medicines for their therapeutic activities, such as antibiotic, antitumor, anti-inflammatory, anticancer, antileukemic and immunomodulatory actions. This investigation evaluates the anti-invasive, antiproliferative and cytotoxic effects of Pleurotus ostreatus (Pleurotaceae) on leukemia cell lines.
Methods:
The proliferation of KG-1 cells was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after treatment with gradient dilutions of P. ostreatus extract. Then, the minimum inhibitory concentration (MIC) of the extract was determined. Moreover, the proliferation of Jurkat cells and bone marrow mesenchymal stem cells (BMSCs), a cancerous cell line and normal body cells, respectively, was considered. The apoptotic morphology of treated KG-1 cells was evaluated with Giemsa staining. The invasion and migration of cells were evaluated using transwell invasion assay. Thereafter, the rates of apoptosis and necrosis were measured by using flow cytometry, and BAX and MMP-9 gene expression were evaluated using quantitative reverse transcription-polymerase chain reaction as apoptotic and metastatic genes, respectively.
Results:
The MIC of the extract was determined to be 1 mg/mL after 48 h. According to the results, the extract decreased the proliferation of leukemia cell lines (KG-1 and Jurkat cells) but had no antiproliferative effects on BMSCs. Moreover, KG-1 cell migration and MMP-9 gene expression decreased after the treatment, and the rate of apoptosis and BAX gene expression increased significantly.
Conclusions:
According to the efficient therapeutic properties of P. ostreatus on leukemia cell lines, this mushroom could be introduced as a natural medicine to cure leukemic patients who suffer from the harmful side effects and enormous costs of chemotherapy.
Acknowledgments
The authors wish to thank Dr. H. Zarremayvan and S. Hosseinilahiji for their very kind assistance.
Author contributions: All the authors have accepted responsibility for the entire content of the submitted manuscript and approved its submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Dhanamani M, Devi SL, Kannan S. Ethnomedicinal plants for cancer therapy – a review. Hygeia J Drugs Med 2011;3:1–10.Search in Google Scholar
2. De Silva DD, Rapior S, Fons F, Bahkali AH, Hyde KD. Medicinal mushrooms in supportive cancer therapies: an approach to anti-cancer effects and putative mechanisms of action. Fungal Divers 2012;55:1–35.10.1007/s13225-012-0151-3Search in Google Scholar
3. Liu Y-W, Mei H-C, Su Y-W, Fan H-T, Chen C-C, Tsai Y-C. Inhibitory effects of Pleurotus tuber-regium mycelia and bioactive constituents on LPS-treated RAW 264.7 cells. J Funct Foods 2014;7: 662–70.10.1016/j.jff.2013.12.019Search in Google Scholar
4. Bassil NM, Abdel-Massih R, El-Chami N, Smith CA, Baydoun E. Pleurotus ostreatus and Ruscus aculeatus extracts cause non-apoptotic Jurkat cell death. J Plant Studies 2012;1:14–24.10.5539/jps.v1n1p14Search in Google Scholar
5. Wu J-Y, Chen C-H, Chang W-H, Chung K-T, Liu Y-W, Lu F-J, et al. Anti-cancer effects of protein extracts from Calvatia lilacina, Pleurotus ostreatus and Volvariella volvacea. Evid Based Complement Alternat Med [Online] 2011;1:10. Available at: http://dx.doi.org/10.1093/ecam/neq057.10.1093/ecam/neq057Search in Google Scholar
6. Kaviyarasan V, Gunasundari D, Radhika K, Dandapani R, Jagadish LK, Venkatakrishnan V, et al. Antioxidant and antiproliferative effect of Pleurotus ostreatus. J Phytol 2009;2:22–8.Search in Google Scholar
7. Lavi I, Friesem D, Geresh S, Hadar Y, Schwartz B. An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells. Cancer Lett 2006;244:61–70.10.1016/j.canlet.2005.12.007Search in Google Scholar PubMed
8. Jedinak A, Sliva D. Pleurotus ostreatus inhibits proliferation of human breast and colon cancer cells through p53-dependent as well as p53-independent pathway. Int J Oncol 2008;33:1307–13.Search in Google Scholar PubMed
9. Polyakov VY, Kir’yanov G, Gerasimenya V, Orlov A, Lazareva E, Murasheva M, et al. Synergistic effects of mycelial fungus (Pleurotes ostreatus) extracts and some cytostatic drugs on proliferation and apoptosis in transformed human cells. Biol Membr 2007;1:301–9.10.1134/S1990747807040058Search in Google Scholar
10. Cohen R, Persky L, Hadar Y. Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol 2002;58:582–94.10.1007/s00253-002-0930-ySearch in Google Scholar PubMed
11. Park S, Picard F, Dreyfus F. Erythroleukemia: a need for a new definition. Leukemia 2002;16:1399–401.10.1038/sj.leu.2402549Search in Google Scholar PubMed
12. Kowal-Vern A, Mazzella FM, Cotelingam JD, Shrit MA, Rector JT, Schumacher HR. Diagnosis and characterization of acute erythroleukemia subsets by determining the percentages of myeloblasts and proerythroblasts in 69 cases. Am J Hematol 2000;65:5–13.10.1002/1096-8652(200009)65:1<5::AID-AJH2>3.0.CO;2-USearch in Google Scholar PubMed
13. Grogan SP, Aklin B, Frenz M, Brunner T, Schaffner T, Mainil-Varlet P. In vitro model for the study of necrosis and apoptosis in native cartilage. J Pathol 2002;198:5–13.10.1002/path.1169Search in Google Scholar
14. Rastogi RP, Sinha RP. Apoptosis: molecular mechanisms and pathogenicity. EXCLI J 2010;8:155–81.Search in Google Scholar
15. Saraste A, Pulkki K. Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 2000;45:528–37.10.1016/S0008-6363(99)00384-3Search in Google Scholar PubMed
16. Mukherjee S, Manna A, Mazumdar M, Das T. Matrix metalloproteinases in cancer metastasis: an unsolved mystery. In: Chakraborti S, Dhalla, Naranjan S, editors. Role of proteases in cellular dysfunction. West Bengal: Springer, 2014:187–212.10.1007/978-1-4614-9099-9_10Search in Google Scholar
17. Egeblad M, Werb Z. New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer 2002;2:161–74.10.1038/nrc745Search in Google Scholar PubMed
18. Sun Y, Dong L. Role of matrix metalloproteinases in the pathogenesis and therapy of leukemia. J Exp Hematol/Chin Assoc Pathophysiol 2003;11:316–20.Search in Google Scholar
19. Labrie M, St-Pierre Y. Epigenetic regulation of mmp-9 gene expression. Cell Mol Life Sci 2013;70:3109–24.10.1007/s00018-012-1214-zSearch in Google Scholar PubMed
20. Reshetnikov SV, Tan K-K. Higher basidiomycota as a source of antitumor and immunostimulating polysaccharides (review). Int J Med Mushr 2001;3:1–34.10.1615/IntJMedMushr.v3.i4.80Search in Google Scholar
21. Olufemi AE, Terry AO, Kola OJ. Anti-leukemic and immunomodulatory effects of fungal metabolites of Pleurotus pulmonarius and Pleurotus ostreatus on benzene-induced leukemia in Wister rats. Korean J Hematol 2012;47:67–73.10.5045/kjh.2012.47.1.67Search in Google Scholar PubMed PubMed Central
22. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell 2011;144:646–74.10.1016/j.cell.2011.02.013Search in Google Scholar PubMed
23. Zhu T, Kim S-H, Chen C-Y. A medicinal mushroom: Phellinus linteus. Curr Med Chem 2008;15:13305.10.2174/092986708784534929Search in Google Scholar PubMed
24. Lin C-H, Sheu G-T, Lin Y-W, Yeh C-S, Huang Y-H, Lai Y-C, et al. A new immunomodulatory protein from Ganoderma microsporum inhibits epidermal growth factor mediated migration and invasion in A549 lung cancer cells. Process Biochem 2010;45:1537–42.10.1016/j.procbio.2010.06.006Search in Google Scholar
25. Huang G-J, Yang C-M, Chang Y-S, Amagaya S, Wang H-C, Hou W-C, et al. Hispolon suppresses SK-Hep1 human hepatoma cell metastasis by inhibiting matrix metalloproteinase-2/9 and urokinase-plasminogen activator through the PI3K/Akt and ERK signaling pathways. J Agric Food Chem 2010;58:9468–75.10.1021/jf101508rSearch in Google Scholar PubMed
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Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Seizure vulnerability and anxiety responses following chronic co-administration and acute withdrawal of caffeine and ethanol in a rat model
- Cardiovascular Function
- Indexes of the erythropoietin level in the blood plasma of chronic heart failure patients with anemia
- Oxidative Stress
- Ameliorative effect of gallic acid on doxorubicin-induced cardiac dysfunction in rats
- Sildenafil, a phosphodiesterase-5 inhibitor, offers protection against carbon tetrachloride-induced hepatotoxicity in rat
- Metabolism
- Antidiabetic potential of polyphenolic-rich fraction of Tamarindus indica seed coat in alloxan-induced diabetic rats
- Effect of L-carnitine on diabetes-induced changes of skeletal muscles in rats
- Inflammation
- Swimming, but not vitamin E, ameliorates prothrombotic state and hypofibrinolysis in a rat model of nonalcoholic fatty liver disease
- Infection
- In vitro ovicidal activity of poly lactic acid curcumin-nisin co-entrapped nanoparticle against Fasciola spp. eggs and its reproductive toxicity
- Phytotherapy
- Assessment of the antioxidant, thrombolytic, analgesic, anti-inflammatory, antidepressant and anxiolytic activities of leaf extracts and fractions of Tetracera sarmentosa (L.) Vahl
- Anti-invasive and antiproliferative effects of Pleurotus ostreatus extract on acute leukemia cell lines
- Letter to the Editor
- Is antiarrhythmic drug effectiveness stable? Extrapolating ICD in different space weather conditions
- Acknowledgment
