Protective effect of Scutellaria species on AAPH-induced oxidative damage in human erythrocyte
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Sasidharan Salini
Abstract
Background:Scutellaria baicalensis is a well-known plant in traditional Chinese medicine. Recently, several Scutellaria species with therapeutic potential have been recognized worldwide. Scutellaria colebrookiana and Scutellaria violacea, native to the Western Ghats of India, are reported to possess free radical scavenging efficacy. At present, the protective effect of these Scutellaria spp. against 2,2′ azobis (2-amidinopropane) hydrochloride (AAPH)-induced oxidative damage in human erythrocytes has been analyzed.
Methods: Oxidative stress in erythrocyte was induced by AAPH. The inhibition of hemolysis, membrane lipid peroxidation, and protein damage by chloroform extracts of Scutellaria spp. was assessed biochemically. Phytochemicals of the extracts were analyzed by Fourier transform infrared spectrophotometer (FTIR).
Results: Approximately 95% of erythrocytes were lysed by AAPH over 3 h of incubation. Significant reduction in hemolysis was observed by the extracts, and the IC50 values were 18.3 and 23.5 μg/mL for S. colebrookiana and S. violacea, respectively. Both the extracts were found to inhibit AAPH-induced lipid peroxidation in ghost membrane with IC50 92±2.8 and 70±5.6 μg/mL. In the analysis of the membrane proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the AAPH-induced degradation of actin was found reduced by both the extracts. The FTIR spectrum revealed the presence of polyphenols, carboxylic acids, alkanes, and aromatic compounds in extracts. In quantitative analysis, the total polyphenolic content estimated was 380±0.23 and 203.7±1.4 mg of gallic acid equivalent per gram extract of S. colebrookiana and S. violacea.
Conclusions: Results indicate that S. colebrookiana and S. violacea are capable of protecting erythrocytes from oxidative damage. This cytoprotective effect of the extract is possibly by its antioxidant property.
Acknowledgments
The authors are grateful to the Board of Research in Nuclear Sciences (BRNS), Department of Atomic Energy (DAE), Government of India (Grant/Award Number: ‘2013/35/11/BRNS/25 dt. 01/04/2013’), for providing the financial support for this work.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Authors’ conflict of interest disclosure: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Reviews
- Assessment of paediatric pain: a critical review
- Can genotype determine the sports phenotype? A paradigm shift in sports medicine
- Oxidative Stress
- Neuroprotective effect of Decalepis hamiltonii on cyclophosphamide-induced oxidative stress in the mouse brain
- Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats
- Metabolism
- Glucose absorption in the intestine of albino rats
- Inflammation
- Antinociceptive and anti-inflammatory potentials of kolaviron: mechanisms of action
- Efficacy of oral vs. intratympanic corticosteroids in sudden sensorineural hearing loss
- Evaluation of antinociceptive and anti-inflammatory properties of methanolic crude extract of Lophopetalum javanicum (bark)
- Anti-nociceptive and anti-inflammatory effects of Withania somnifera root in fructose fed male rats
- Phytotherapy
- Biflavonoid fraction from Garcinia kola seed ameliorates hormonal imbalance and testicular oxidative damage by anti-tuberculosis drugs in Wistar rats
- Protective effect of Scutellaria species on AAPH-induced oxidative damage in human erythrocyte
- Miscellaneous
- Role of monocyte chemoattractant protein-1, stromal derived factor-1 and retinoic acid in pathophysiology of neuropathic pain in rats
- Hormonal and organ-specific dysfunction induced by the interaction between titanium dioxide nanoparticles and salicylic acid in male mice
- Case Report
- Blood pressure variability and baroreflex sensitivity of a healthy male during cold pressor test that induced development of neurocardiogenic syncope
Articles in the same Issue
- Frontmatter
- Reviews
- Assessment of paediatric pain: a critical review
- Can genotype determine the sports phenotype? A paradigm shift in sports medicine
- Oxidative Stress
- Neuroprotective effect of Decalepis hamiltonii on cyclophosphamide-induced oxidative stress in the mouse brain
- Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats
- Metabolism
- Glucose absorption in the intestine of albino rats
- Inflammation
- Antinociceptive and anti-inflammatory potentials of kolaviron: mechanisms of action
- Efficacy of oral vs. intratympanic corticosteroids in sudden sensorineural hearing loss
- Evaluation of antinociceptive and anti-inflammatory properties of methanolic crude extract of Lophopetalum javanicum (bark)
- Anti-nociceptive and anti-inflammatory effects of Withania somnifera root in fructose fed male rats
- Phytotherapy
- Biflavonoid fraction from Garcinia kola seed ameliorates hormonal imbalance and testicular oxidative damage by anti-tuberculosis drugs in Wistar rats
- Protective effect of Scutellaria species on AAPH-induced oxidative damage in human erythrocyte
- Miscellaneous
- Role of monocyte chemoattractant protein-1, stromal derived factor-1 and retinoic acid in pathophysiology of neuropathic pain in rats
- Hormonal and organ-specific dysfunction induced by the interaction between titanium dioxide nanoparticles and salicylic acid in male mice
- Case Report
- Blood pressure variability and baroreflex sensitivity of a healthy male during cold pressor test that induced development of neurocardiogenic syncope
