Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats
-
Ramalingam Sripradha
and
Nachimuthu Maithilikarpagaselvi
Abstract
Background: Excess fructose consumption causes dyslipidemia, oxidative stress, and various complications. Hydroxycitric acid (HCA), one of the principal components of the fruit Garcinia cambogia, has been shown to possess antiobesity properties. The objective was to investigate the effects of HCA on redox imbalance and activation of stress sensitive kinases in high fructose-fed rats.
Methods: Male Wistar rats (n=40) were randomly divided into four groups with 10 rats in each group. The rats were fed with either standard rodent diet or 60% fructose diet and administered with HCA at a dose of 400 mg/kg body wt/day for 10 weeks. Body weight was measured once a week, and food intake was noted daily. At the end of the study, lipid profile and oxidative stress parameters were estimated. Expressions of stress sensitive kinases were analyzed in liver homogenates.
Results: Fructose-fed rats displayed elevated body weight, higher levels of plasma total cholesterol (TC), triacylglycerol (TAG), non-high-density lipoprotein cholesterol (non HDL-C), malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), lower levels of HDL-C, glutathione (GSH), glutathione peroxidase (GPx), and total antioxidant status (TAS). Fructose feeding caused higher phosphorylation of stress sensitive kinases ERK ½ and p38. Administration with HCA lowered body weight, food intake, TAG, non-HDL-C, MDA, TOS, and OSI and elevated GSH, GPx, and TAS levels. Reduced phosphorylation of ERK ½ and p38 mitogen-activated protein kinase (MAPK) was observed upon HCA treatment.
Conclusions: Thus, HCA improved fructose induced redox imbalance and activation of stress sensitive kinases through its hypolipidemic effects.
Acknowledgments
We thank Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) for providing the necessary infrastructure for the study. We thank Dr. A.G. Ramachandran Nair, formerly Professor of Chemistry in Pondicherry University, for his help in conceptualizing this research project. We also thank Indfrag Limited, Bangalore, India, for their assistance in providing (-) hydroxycitric acid and its analytical work.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Reviews
- Assessment of paediatric pain: a critical review
- Can genotype determine the sports phenotype? A paradigm shift in sports medicine
- Oxidative Stress
- Neuroprotective effect of Decalepis hamiltonii on cyclophosphamide-induced oxidative stress in the mouse brain
- Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats
- Metabolism
- Glucose absorption in the intestine of albino rats
- Inflammation
- Antinociceptive and anti-inflammatory potentials of kolaviron: mechanisms of action
- Efficacy of oral vs. intratympanic corticosteroids in sudden sensorineural hearing loss
- Evaluation of antinociceptive and anti-inflammatory properties of methanolic crude extract of Lophopetalum javanicum (bark)
- Anti-nociceptive and anti-inflammatory effects of Withania somnifera root in fructose fed male rats
- Phytotherapy
- Biflavonoid fraction from Garcinia kola seed ameliorates hormonal imbalance and testicular oxidative damage by anti-tuberculosis drugs in Wistar rats
- Protective effect of Scutellaria species on AAPH-induced oxidative damage in human erythrocyte
- Miscellaneous
- Role of monocyte chemoattractant protein-1, stromal derived factor-1 and retinoic acid in pathophysiology of neuropathic pain in rats
- Hormonal and organ-specific dysfunction induced by the interaction between titanium dioxide nanoparticles and salicylic acid in male mice
- Case Report
- Blood pressure variability and baroreflex sensitivity of a healthy male during cold pressor test that induced development of neurocardiogenic syncope
Articles in the same Issue
- Frontmatter
- Reviews
- Assessment of paediatric pain: a critical review
- Can genotype determine the sports phenotype? A paradigm shift in sports medicine
- Oxidative Stress
- Neuroprotective effect of Decalepis hamiltonii on cyclophosphamide-induced oxidative stress in the mouse brain
- Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats
- Metabolism
- Glucose absorption in the intestine of albino rats
- Inflammation
- Antinociceptive and anti-inflammatory potentials of kolaviron: mechanisms of action
- Efficacy of oral vs. intratympanic corticosteroids in sudden sensorineural hearing loss
- Evaluation of antinociceptive and anti-inflammatory properties of methanolic crude extract of Lophopetalum javanicum (bark)
- Anti-nociceptive and anti-inflammatory effects of Withania somnifera root in fructose fed male rats
- Phytotherapy
- Biflavonoid fraction from Garcinia kola seed ameliorates hormonal imbalance and testicular oxidative damage by anti-tuberculosis drugs in Wistar rats
- Protective effect of Scutellaria species on AAPH-induced oxidative damage in human erythrocyte
- Miscellaneous
- Role of monocyte chemoattractant protein-1, stromal derived factor-1 and retinoic acid in pathophysiology of neuropathic pain in rats
- Hormonal and organ-specific dysfunction induced by the interaction between titanium dioxide nanoparticles and salicylic acid in male mice
- Case Report
- Blood pressure variability and baroreflex sensitivity of a healthy male during cold pressor test that induced development of neurocardiogenic syncope
