Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats
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Luqman A. Olayaki
Abstract
Background: Glucose-lowering effects of Moringa oleifera extracts have been reported. However, the mechanism for its hypoglycemic effects is not yet understood. This study investigated the effect of oral administration of methanolic extracts of M. oleifera (MOLE) on glucose tolerance, glycogen synthesis, and lipid metabolism in rats with alloxan-induced diabetes.
Methods: MOLE was screened for key phytochemicals and its total flavonoids and phenolic contents were quantified. Diabetes was induced by intraperitoneal injection of 120 mg/kg BW alloxan. Normal and diabetic control rats received saline, while rats in other groups received 300 or 600 mg/kg body weight of MOLE or metformin (100 mg/kg body weight of metformin) for 6 weeks. Food intake and body weight were monitored throughout the experiment. Intraperitoneal glucose tolerance was assessed and serum glucose, insulin, and lipids were measured at the end of the experiment. Liver and muscle glycogen synthase activities, glycogen content, and glucose uptake were determined.
Results: Administration of MOLE did not affect food intake but inhibited weight loss, significantly (p<0.01) improved glucose tolerance, and increased serum insulin levels by 1.3–1.7-fold (p<0.01). MOLE treatment significantly (p<0.001) reduced serum concentrations of triglyceride, total cholesterol, and low-density lipoprotein (LDL)-cholesterol and enhanced serum level of high-density lipoprotein (HDL) by 2.4- to 3.2-fold (p<0.001). Glycogen synthase activities and glycogen contents were higher in MOLE-treated rats compared with rats receiving metformin or saline and the extract improved glucose uptake by 49%–59% (p<0.01).
Conclusions: These results showed that hypoglycemic effects of MOLE might be mediated through the stimulation of insulin release leading to enhanced glucose uptake and glycogen synthesis.
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©2015 by De Gruyter
Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Neurobehavioural evaluation of Lophira alata (Ochnaceae) stem bark extract in mice
- Cardiovascular Function
- Altered cardiovascular autonomic function in young normotensive offspring of hypertensive parents – Is obesity an additional risk factor?
- Atheromic and lymphoplasmacytic effects of Mangifera indica methanolic leaf extract on the heart of chinchilla rabbits
- Cardiovascular-Pulmonary Interactions
- Prevalence of cardiovascular risk factors and management practices of acute coronary syndrome in a tertiary care hospital
- Oxidative Stress
- Effect of homopterocarpin, an isoflavonoid from Pterocarpuserinaceus, on indices of liver injury and oxidative stress in acetaminophen-provoked hepatotoxicity
- Metabolism
- Activation of insulin signaling and energy sensing network by AICAR, an AMPK activator in insulin resistant rat tissues
- Mature coconut water exhibits antidiabetic and antithrombotic potential via L-arginine-nitric oxide pathway in alloxan induced diabetic rats
- Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats
- Morphological changes in the pancreas and glucose reduction of the aqueous extract of Costus afer leaf on alloxan-induced diabetic rats
- Immune Response
- Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats
- Immune response modulatory effect of Emilia sonchifolia (L.) DC: an in vivo experimental study
- Inflammation
- Thymoquinone ameliorates NLRP3-mediated inflammation in the pancreas of albino Wistar rats fed ethanol and high-fat diet
- Miscellaneous
- Perceptions and practices of self-medication in healthcare and nonhealthcare university students in South India
Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Neurobehavioural evaluation of Lophira alata (Ochnaceae) stem bark extract in mice
- Cardiovascular Function
- Altered cardiovascular autonomic function in young normotensive offspring of hypertensive parents – Is obesity an additional risk factor?
- Atheromic and lymphoplasmacytic effects of Mangifera indica methanolic leaf extract on the heart of chinchilla rabbits
- Cardiovascular-Pulmonary Interactions
- Prevalence of cardiovascular risk factors and management practices of acute coronary syndrome in a tertiary care hospital
- Oxidative Stress
- Effect of homopterocarpin, an isoflavonoid from Pterocarpuserinaceus, on indices of liver injury and oxidative stress in acetaminophen-provoked hepatotoxicity
- Metabolism
- Activation of insulin signaling and energy sensing network by AICAR, an AMPK activator in insulin resistant rat tissues
- Mature coconut water exhibits antidiabetic and antithrombotic potential via L-arginine-nitric oxide pathway in alloxan induced diabetic rats
- Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats
- Morphological changes in the pancreas and glucose reduction of the aqueous extract of Costus afer leaf on alloxan-induced diabetic rats
- Immune Response
- Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats
- Immune response modulatory effect of Emilia sonchifolia (L.) DC: an in vivo experimental study
- Inflammation
- Thymoquinone ameliorates NLRP3-mediated inflammation in the pancreas of albino Wistar rats fed ethanol and high-fat diet
- Miscellaneous
- Perceptions and practices of self-medication in healthcare and nonhealthcare university students in South India
