The Dependence of the Copper Dissociation Rate Constants from Human(Serum)- and Ονο-Transferrin on pH and the Anions
-
Junzo Hirose
,
Isao Fujiwara
Abstract
Transferrin is an iron carrier protein. It is known that the conformational change in the protein moiety induced by binding of nonsynergistic anions influences the metal release rate constants from metal transferrins. The copper dissociation rate constants of dicupric human(serum)-transferrin and ovo-transferrin were measured under the conditions of various pHs and ionic strengths to estimate the residues which interact with nonsynergistic anions. The pH and the ionic strength dependence of the copper dissociation rate constants in the N-lobe of serum (human)-transferrin and both lobes of ovo-Tf clearly show that some residue which has a pKa around 6.0 is involved in the binding of nonsynergistic anions and accelerating the copper dissociation rate. In the C-lobe of serum(human)-Tf, the copper dissociation rate constants were almost independent of pH between pH 7.0 and 9.0, but the dissociation rates were constantly accelerated by the presence of 0.1 Μ KCl. This behavior suggests that the residue in the C-lobe of serum(human)-Tf is constantly protonated between pH 7.0 and 9.0 and is involved in the binding of nonsynergistic anions binding site.
© 2014 by Walter de Gruyter Berlin/Boston
Artikel in diesem Heft
- Tribute
- Kinetics of Dissociation of the Cobalt(II) and Copper(II) Complexes of 2,2′,2″-tris(monomethylamino)triethylamine in Aqueous Acidic Solution
- The Influence of Steric Effects on the Oxidation Kinetics of [FeII(aminopolycarboxylato)] Complexes with Dioxygen
- The Hydrolysis Kinetics of ds-diamminedichloroplatinum(II). 10. Activation Volumes for Halide Anation of Some czs-[Pt(N)2(OH2)2]2+ Systems
- The Dependence of the Copper Dissociation Rate Constants from Human(Serum)- and Ονο-Transferrin on pH and the Anions
- Kinetics of the Photosubstitution of ds-Bis(benzonitrile)dichloroplatinum(II) in Chloroform
- Kinetics and Mechanisms of the Reaction of Aluminium(III) with Polyaminocarboxylic Acids
- Kinetics and Mechanism of Oxidation of Tellurium(IV) by Alkaline Diperiodatonickelate(IV)
- Complexation of Pendant Arm Macrocycles with Cu(II) Hydroxo Complexes in Strongly Alkaline Solutions. Investigation of pH Dependences
- Kinetics and Mechanism of the Chromium(VI) Oxidation of Formic Acid in the Presence of Picolinic Acid and in the Presence and Absence of Surfactants
- Kinetics and Mechanism of Chromium(III) Catalysed Oxidation of Propan-1-ol and Butan-1-ol by Cerium(IV) in Aqueous Sulfuric acid Media
- Notes for Contributors. Inorganic Reaction Mechanisms
- Journal Order Form
Artikel in diesem Heft
- Tribute
- Kinetics of Dissociation of the Cobalt(II) and Copper(II) Complexes of 2,2′,2″-tris(monomethylamino)triethylamine in Aqueous Acidic Solution
- The Influence of Steric Effects on the Oxidation Kinetics of [FeII(aminopolycarboxylato)] Complexes with Dioxygen
- The Hydrolysis Kinetics of ds-diamminedichloroplatinum(II). 10. Activation Volumes for Halide Anation of Some czs-[Pt(N)2(OH2)2]2+ Systems
- The Dependence of the Copper Dissociation Rate Constants from Human(Serum)- and Ονο-Transferrin on pH and the Anions
- Kinetics of the Photosubstitution of ds-Bis(benzonitrile)dichloroplatinum(II) in Chloroform
- Kinetics and Mechanisms of the Reaction of Aluminium(III) with Polyaminocarboxylic Acids
- Kinetics and Mechanism of Oxidation of Tellurium(IV) by Alkaline Diperiodatonickelate(IV)
- Complexation of Pendant Arm Macrocycles with Cu(II) Hydroxo Complexes in Strongly Alkaline Solutions. Investigation of pH Dependences
- Kinetics and Mechanism of the Chromium(VI) Oxidation of Formic Acid in the Presence of Picolinic Acid and in the Presence and Absence of Surfactants
- Kinetics and Mechanism of Chromium(III) Catalysed Oxidation of Propan-1-ol and Butan-1-ol by Cerium(IV) in Aqueous Sulfuric acid Media
- Notes for Contributors. Inorganic Reaction Mechanisms
- Journal Order Form
