Startseite Investigation of Humulus lupulus as a novel adsorbent for protein adsorption: assessment of sorption kinetics, surface topology, and thermal properties using BSA as a model protein
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Investigation of Humulus lupulus as a novel adsorbent for protein adsorption: assessment of sorption kinetics, surface topology, and thermal properties using BSA as a model protein

  • Tripti Singh und Ashwani Mathur EMAIL logo
Veröffentlicht/Copyright: 1. Mai 2025
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International Journal of Materials Research
Aus der Zeitschrift International Journal of Materials Research Band 116 Heft 5

Abstract

The increasing production of protein-rich waste, primarily from the household and food industries, associated with the growing global population has imposed a negative environmental burden on society at large. Protein waste is a growing international concern, with more unsegregated waste released from developing countries. This work explored the sorption behavior of amorphous plant extract of Humulus lupulus, towards bovine serum albumin as a representative model protein. The adsorbed proteins analyzed using scanning electron microscopy, revealed irregular beads masking the surface cues and changing the surface smoothness, further validated by atomic force microscopy. The atomic force microscopy analysis showed an increase in the surface potential of pre-adsorbed material (−60.2 mV to 42.5 mV) compared to post-adsorbed complex (−736 mV to 640 mV) suggesting the protein interaction on the plant surface. Differential scanning calorimetry indicated the possible interaction between protein and surface which is responsible for showing a shift in the enthalpy pattern of the surface pre- and post-adsorption. Change in enthalpy pattern, higher surface potential, and shift in Fourier-transform infrared spectroscopy binding pattern indicates an interaction between plant surface and protein that was further validated and confirmed using the adsorption isotherm. The adsorption isotherm towards bovine serum albumin protein followed the Freundlich isotherm with k and n values of 1.143 and 1.157, respectively. The Freundlich isotherm pattern observed for the material has suggested H. lupulus plant extract as a suitable surface for protein adsorption. The study opens the avenue for the adsorption kinetics of different proteins and establishing the plant extract as a suitable remediation solution for environmental sustainability.

Keywords: Humulus lupulus ; Protein; Adsorption; Sustainability; Scanning electron microscopy; Atomic force microscopy
Correspondonding author: Ashwani Mathur, Department of Biotechnology, Jaypee Institute of Information Technology, Noida A-10, Sector-62, Noida-201309, Uttar Pradesh, India, E-mail:

Acknowledgments

The authors acknowledge the Council of Scientific and Industrial Research–Central Electronics Engineering Research Institute(CSIR–CEERI), Pilani, Rajasthan, Central Research Facility (CRF), Indian Institute of Technology (IIT), Delhi, Department of Textile and Fibre Engineering, Indian Institute of Technology (IIT), Delhi, for providing all the necessary support.

  1. Research ethics: Not applicable.

  2. Informed consent: Not applicable.

  3. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: The authors state no conflict of interest.

  6. Research funding: None declared.

  7. Data availability: Not applicable.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/ijmr-2023-0346).

Received: 2023-11-22
Accepted: 2024-09-17
Published Online: 2025-05-01
Published in Print: 2025-05-26

© 2025 Walter de Gruyter GmbH, Berlin/Boston

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  17. News
  18. DGM – Deutsche Gesellschaft für Materialkunde
https://doi.org/10.1515/ijmr-2023-0346
Schlagwörter für diesen Artikel
Humulus lupulus; Protein; Adsorption; Sustainability; Scanning electron microscopy; Atomic force microscopy

Artikel in diesem Heft

  1. Frontmatter
  2. Editorial
  3. ICEAM 2023 and ICHEAM-2024
  4. Reviews
  5. Piezo-photocatalyst: unveiling unique catalytic properties of piezoelectric materials for photoreduction of CO2 – a review
  6. Transforming biomass into batteries: harnessing cellulose and nanocellulose for a sustainable energy storage future
  7. Original Papers
  8. Enhanced photocatalytic activity and dye degradation efficiency of La doped BiFeO3–reduced graphene oxide nanocomposite
  9. Investigation on structural, optical, thermal, and magnetic properties of BiFeO3 nanoparticles synthesized at lower annealing temperature
  10. Design and optimization of an economic HTL-free, non-toxic double-layer perovskite solar cell for enhanced performance and stability
  11. Analysis of high pressure response of nano-TiO2 for anatase and rutile phase
  12. Tin (Sn) nanoparticles: novel synthesis by exploding wire technique and crystalline, optical properties
  13. Effect of nanowire curviness on the resistance of nanowire-based networks: a computational study
  14. Determination of yield and BET surface area on varying microwave power, radiation time and flow rate of nitrogen gas during pyrolysis of mustard husk (Brassica juncea)
  15. Enhanced first-order non-linear optical responses of 4-amino-6-chloro-1,3-benzenedisulfonamide polymer
  16. Investigation of Humulus lupulus as a novel adsorbent for protein adsorption: assessment of sorption kinetics, surface topology, and thermal properties using BSA as a model protein
  17. News
  18. DGM – Deutsche Gesellschaft für Materialkunde
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