Different Damage to the Mechanical Barrier Function of IPEC-J2 Induced by Soybean Allergen β-conglycinin Hydrolyzed Peptides
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Abstract
Three major enzyme-hydrolyzed peptides have been produced after simulative digestion in vitro of soybean β-conglycinin. The intestinal barrier of IPEC-J2 induced by β-conglycinin enzyme-hydrolyzed peptides was evaluated in this study. The increased alkaline phosphatase (AP) activity was actually linearly correlated with the incubation time by the hydrolysate, the purified 52 kD peptide, or the mixture of 25 and 30 kD peptides. The MTT and TEER values declined in dose-dependence (0–2 mg/mL, p \lt 0.05) or in time-dependence (2–24 h, p \lt 0.05). After treatment with different hydrolyzed peptides, the tight junction expression of claudin-3, claudin-4, occludin, and ZO-1 were reduced (p \lt 0.05). Finally, it is found out that the maximum damage to the epithelial barrier function was induced by the mixture of 25 and 30 kD peptide, whereas the minimum damage was caused by the 52 kD peptide.
Funding statement: This work was financially supported by the National Natural Science Foundation of China (No. 31572439 and No.31572415), the Natural Science Foundation of Jilin Province of China (No. 20160101348JC), “Twelfth Five-year Plan”for Sci & Tech Research Program of Jilin Education Department of P.R. China (No.2015198).
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Artikel in diesem Heft
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Artikel in diesem Heft
- Research Articles
- Partitioning Behavior of Lysozyme and α-lactalbumin in Aqueous Two-Phase System Formed by Ionic Liquids and Potassium Phosphate
- Effects of Electrolyte Concentration and Ultrasound Pretreatment on Ohmic-Assisted Hydrodistillation of Essential Oils from Mentha piperita L.
- Valorisation of the Brewers’ Spent Grain Through Sourdough Bread Making
- Effect of High Temperature Intermittent Drying on Rice Seed Viability and Vigor
- Numerical Simulation on Superheated Steam Fluidized Bed Drying at Different Operating Pressures
- Different Damage to the Mechanical Barrier Function of IPEC-J2 Induced by Soybean Allergen β-conglycinin Hydrolyzed Peptides
- Controlled Release of Salidroside Microspheres Prepared Using a Chitosan and Methylcellulose Interpenetrating Polymer Network
- Application of Natural Frequencies for Prediction of Apple Texture Based on Partial Least Squares Regression
- Comparison of Different Physical Technique-Assisted Alkali Methods for the Extraction of Rice Bran Protein and its Characterizations
- Short Communication
- Improvement of Air Homogeneity in Paddy Dryer With Central Air Flow Channel
