Purification, Characterization, Antioxidant and Antitumour Activities of Polysaccharides from Apple Peel Pomace Obtained by Pre-pressing Separation
Abstract
Purification, characterization, in vitro antioxidant and antitumour activities of polysaccharides from apple peel pomace obtained by pre-pressing separation were investigated. Apple peel polysaccharides (APP) were obtained by water extraction and ethanol precipitation. They were further purified by chromatography of Cellulose DEAE-52 and Sephadex G-150 to obtain three fractions (APP-1, APP-2 and APP-3). Both GC-MS and HPLC analysis showed that galactose and arabinose were two main components in the three fractions with an average molecular weight of 197 kDa, 395 kDa and 303 kDa, respectively. Uronic acids were only presented in APP-2 and APP-3, which were further confirmed by Fourier transform infrared spectroscopy. Besides, the results showed that the antioxidant activity measured by ferric-reducing power and scavenging of O2•−, DPPH• and OH• and the antitumour activity examined by HepG2 cells were connected with the content of uronic acids. These findings provide scientific basis for comprehensive utilization of apple peel pomace.
Acknowledgements
This research is sponsored by China Agricultural Research System (CARS-28) from Ministry of Agriculture of People’s Republic of China
References
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© 2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Articles
- Sensory Characteristics of Maillard Reaction Products Obtained from Sunflower Protein Hydrolysates and Different Sugar Types
- Purification, Characterization, Antioxidant and Antitumour Activities of Polysaccharides from Apple Peel Pomace Obtained by Pre-pressing Separation
- Effect of Trypsin on Antioxidant Activity and Gel-Rheology of Flaxseed Protein
- Analysis about Heat Transfer of Vegetables during Cold Shock Treatment and Preservation Quality after Storage
- Thermal Natural Convection Analysis of Olive Oil in Different Cookware Materials for Induction Stoves
- Enhancement of Gel Properties of Sardine Surimi using Squid Ink Tyrosinase in Combination with Coconut Husk Extract
- Effect of Different Drying Techniques on Physicochemical, Micro-structural and Bioactive Characteristics of Barberry Milk Smoothie Powder
- Antioxidant Activity Improvement and Evaluation of Structure Changes of SHECN Treated by Pulsed Electric Field (PEF) Technology
- Optimization of Total Monomeric Anthocyanin (TMA) and Total Phenolic Content (TPC) Extractions from Red Cabbage (Brassica oleracea var. capitata f. rubra): Response Surface Methodology versus Artificial Neural Network
- Quality Improvement of Canned Fish with the Use of Cinnamon Oil Extract
Articles in the same Issue
- Articles
- Sensory Characteristics of Maillard Reaction Products Obtained from Sunflower Protein Hydrolysates and Different Sugar Types
- Purification, Characterization, Antioxidant and Antitumour Activities of Polysaccharides from Apple Peel Pomace Obtained by Pre-pressing Separation
- Effect of Trypsin on Antioxidant Activity and Gel-Rheology of Flaxseed Protein
- Analysis about Heat Transfer of Vegetables during Cold Shock Treatment and Preservation Quality after Storage
- Thermal Natural Convection Analysis of Olive Oil in Different Cookware Materials for Induction Stoves
- Enhancement of Gel Properties of Sardine Surimi using Squid Ink Tyrosinase in Combination with Coconut Husk Extract
- Effect of Different Drying Techniques on Physicochemical, Micro-structural and Bioactive Characteristics of Barberry Milk Smoothie Powder
- Antioxidant Activity Improvement and Evaluation of Structure Changes of SHECN Treated by Pulsed Electric Field (PEF) Technology
- Optimization of Total Monomeric Anthocyanin (TMA) and Total Phenolic Content (TPC) Extractions from Red Cabbage (Brassica oleracea var. capitata f. rubra): Response Surface Methodology versus Artificial Neural Network
- Quality Improvement of Canned Fish with the Use of Cinnamon Oil Extract