Assessment of Various Pretreatment and Extraction Methods for the Extraction of Bioactive Compounds from Orthosiphon stamineus Leaf via Microstructures Analysis
-
Tiam You See
and
Gek Cheng Ngoh
Abstract
The impacts of various methods such as mechanical grinding, ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE), and also sample pretreatments using acid and alkali on the microstructure of plant sample were studied for the extraction of bioactive compounds from Orthosiphon stamineus leaf. From scanning electron microscopy (SEM) analysis of the extracted sample, UAE and MAE induced significant disruption on glandular trichomes structure, which is the main site for biosynthesis of plant secondary metabolites. This improves the diffusion of bioactive compound and resulted in approximately 86–95 % of the total extraction yield quantified by conventional Soxhlet extraction. Chemical pretreatments generally imparted weaker microstructures disruption thus slight improvement on the extraction yields was observed. In this case, acid reagent is more suitable for the pretreatment as the presence of alkali decomposes the bioactive compounds. In a nutshell, the performance of an extraction strongly depends on its degree of disruption on the plant sample microstructure.
Funding statement: This work was performed under the Centre for Separation Science and Technology (CSST), University of Malaya and financially supported through Fundamental Research Grant Scheme (FRGS: FP076-2015A) and University of Malaya Research Grant (UMRG: RP002A-13AET).
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Articles in the same Issue
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- Research Articles
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- Modeling and Optimization of Lipase-Catalyzed Partial Hydrolysis for Diacylglycerol Production in Packed Bed Reactor
- Effect of Ohmic Heating on Polyphenol Oxidase Activity, Electrical and Physicochemical Properties of Fresh Tender Coconut Water
- Incorporation of β-Glucans into Pasta Extracted from Two Tunisian Barley Cultivars
- Assessment of Various Pretreatment and Extraction Methods for the Extraction of Bioactive Compounds from Orthosiphon stamineus Leaf via Microstructures Analysis
Articles in the same Issue
- Frontmatter
- Research Articles
- Colorimetry Technique as a Tool for Determining Release Kinetics and Mass Transfer Parameters of Anthocyanins Encapsulated in W1/O/W2 Double Emulsions
- Image Analysis and High Dimensional Control Chart for Inspection of Sausage Color Homogeneity and Uniformity
- Impact of Encapsulated Lactobacillus casei 01 Along with Pasteurized Purple-Rice Drinks on Modulating Colon Microbiome using a Digestive Model
- Effects of Soy Lecithin Levels and Microfluidization Conditions on Properties of Fish Gelatin-Based Film Incorporated with Palm Oil
- Effect of Temperature on the Drying Behavior and Quality of Citrus Peels
- Preparation of Cross-Linked High Amylose Corn-Starch and Its Effects on Self-Reinforced Starch Films
- Modeling and Optimization of Lipase-Catalyzed Partial Hydrolysis for Diacylglycerol Production in Packed Bed Reactor
- Effect of Ohmic Heating on Polyphenol Oxidase Activity, Electrical and Physicochemical Properties of Fresh Tender Coconut Water
- Incorporation of β-Glucans into Pasta Extracted from Two Tunisian Barley Cultivars
- Assessment of Various Pretreatment and Extraction Methods for the Extraction of Bioactive Compounds from Orthosiphon stamineus Leaf via Microstructures Analysis
