LncRNA MALAT1 inhibits hypoxia/reoxygenation-induced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis
Abstract
Angiogenesis is believed to protect against hypoxia/reoxygenation (H/R)-induced cell injury. MALAT1 and microRNA-320a (miR-320a) are involved in cancer angiogenesis. To investigate the function of the MALAT1/miR-320a axis in H/R-induced cell injury, human umbilical vein endothelial cell (HUVEC) angiogenesis was detected using the Cell Counting Kit-8 (CCK-8), Transwell migration, cell adhesion and tube formation assays. The expression of MALAT1 and miR-320a was revealed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The direct binding relationship between miR-320a and MALAT1 was detected by RNA immunoprecipitation (RIP) and dual luciferase reporter assays. The data indicated that H/R induces angiogenesis injury and that the expression of MALAT1 was augmented in H/R-stimulated HUVECs. Overexpression of MALAT1 alleviated H/R-stimulated HUVEC dysfunction, whereas silencing of MALAT1 exerted the opposite effects. MALAT1 also reduced miR-320a levels in HUVECs. Overexpression of miR-320a repressed the function of MALAT1 on H/R-stimulated HUVECs, whereas inhibition of miR-320a exerted the opposite effect. Additionally, miR-320a inhibition alleviated H/R-stimulated HUVEC injury via RAC1. Taken together, this investigation concluded that MALAT1 represses H/R-stimulated HUVEC injury by targeting the miR-320a/RAC1 axis.
Funding source: National Key Research and Development Program
Award Identifier / Grant number: 2016YFA0100800
Funding source: Major International (Regional) Joint Research Project
Award Identifier / Grant number: 81810001048
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 81873994
Award Identifier / Grant number: 81671105
Funding statement: This work was financially supported by the National Key Research and Development Program (Grant No. 2016YFA0100800), the Major International (Regional) Joint Research Project (Grant No. 81810001048), and the National Natural Science Foundation of China, (Grant Nos. 81873994 and 81671105).
Conflict of interest statement: The authors do not declare any conflicts of interest.
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©2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- Genetic, epigenetic and biochemical regulation of succinate dehydrogenase function
- Transport mechanism of Mycobacterium tuberculosis MmpL/S family proteins and implications in pharmaceutical targeting
- Research Articles/Short Communications
- Genes and Nucleic Acids
- LncRNA MALAT1 inhibits hypoxia/reoxygenation-induced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis
- Protein Structure and Function
- Identification of new probe substrates for human CYP20A1
- Molecular Medicine
- Exosomal MALAT1 derived from ox-LDL-treated endothelial cells induce neutrophil extracellular traps to aggravate atherosclerosis
- Cell Biology and Signaling
- Derlin-1 functions as a growth promoter in breast cancer
- Alterations in rat adipose tissue transcriptome and proteome in response to prolonged fasting
- Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis
- Tumor-suppressive activity of sTRAIL on circulating CD44+ cells in patients with non-small cell lung cancer
Artikel in diesem Heft
- Frontmatter
- Reviews
- Genetic, epigenetic and biochemical regulation of succinate dehydrogenase function
- Transport mechanism of Mycobacterium tuberculosis MmpL/S family proteins and implications in pharmaceutical targeting
- Research Articles/Short Communications
- Genes and Nucleic Acids
- LncRNA MALAT1 inhibits hypoxia/reoxygenation-induced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis
- Protein Structure and Function
- Identification of new probe substrates for human CYP20A1
- Molecular Medicine
- Exosomal MALAT1 derived from ox-LDL-treated endothelial cells induce neutrophil extracellular traps to aggravate atherosclerosis
- Cell Biology and Signaling
- Derlin-1 functions as a growth promoter in breast cancer
- Alterations in rat adipose tissue transcriptome and proteome in response to prolonged fasting
- Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis
- Tumor-suppressive activity of sTRAIL on circulating CD44+ cells in patients with non-small cell lung cancer